The potential of amphiphilic chitosan derivative, namely, N-deoxycholic acid-O-glycol chitosan (DAGC), as a carrier agent for pesticide rotenone was assessed for the first time. The physicochemical properties of DAGC were characterized using several analytical instruments such as 1 H-NMR spectrometer, CHN-O elemental analyzer, FTIR spectrometer, TGA, DSC, and UV-visible spectrophotometer. DAGC formed self-aggregation in the solution with a spherical shape of 67.5-83.3 nm. Rotenone-loaded DAGC could give an encapsulation efficiency of up to 91.69%. DAGC micelles exhibit good control release properties as it can prolong the release of rotenone up to 41 h more than free rotenone. The Korsmeyer-Peppas constant (n) values suggest that rotenone release from DAGC micelles was governed by diffusion and relaxation of DAGC chains. The results from this study conclude that DAGC is viable to be applied as a carrier agent in pesticide formulation.