Use of ultrasonic vocalizations to assess olfactory detection in mouse pups treated with 3-methylindole

Lemasson, Morgane; Delbé, Charles; Gheusi, Gilles; Vincent, Jean‐Didier; Lledo, Pierre−Marie

doi:10.1016/j.beproc.2004.09.001

Cited by 24 publications

(21 citation statements)

References 70 publications

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“…A majority of pups emitted ultrasonic vocalizations at 62-kHz initially, upon separation from their littermates (USVs; Videos S1 and S2) [20]. A period of 10–15 minutes in the dark was sufficient for pups to acclimate to the testing chamber and cease making isolation-induced 62-kHz USVs.…”

Section: Resultsmentioning

confidence: 99%

“…The audio output from the mini-3 detector was recorded continuously onto a sound recorder (Sony PCM-M10). Timing of USVs was detected by thresholding the root mean square levels (5 msec bins; Matlab) as described previously with minor modifications [20]. The Matlab code used for analyses is available from authors upon request.…”

Section: Methodsmentioning

confidence: 99%

“…Neonatal pups at ages P7 to P9 were tested individually, and transferred to the testing chamber under dim red light. Pups were allowed to acclimate to the chamber until the isolation-induced 62-kHz USVs calls [20] ceased (10–15 minutes). A recording trial began with a 60 second baseline in the dark, a 60 second exposure to light, followed by an additional 60 seconds of recording in the dark.…”

Section: Methodsmentioning

confidence: 99%

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Light Evokes Melanopsin-Dependent Vocalization and Neural Activation Associated with Aversive Experience in Neonatal Mice

et al. 2012

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Melanopsin-expressing intrinsically photosensitive retinal ganglion cells (ipRGCs) are the only functional photoreceptive cells in the eye of newborn mice. Through postnatal day 9, in the absence of functional rods and cones, these ipRGCs mediate a robust avoidance behavior to a light source, termed negative phototaxis. To determine whether this behavior is associated with an aversive experience in neonatal mice, we characterized light-induced vocalizations and patterns of neuronal activation in regions of the brain involved in the processing of aversive and painful stimuli. Light evoked distinct melanopsin-dependent ultrasonic vocalizations identical to those emitted under stressful conditions, such as isolation from the litter. In contrast, light did not evoke the broad-spectrum calls elicited by acute mechanical pain. Using markers of neuronal activation, we found that light induced the immediate-early gene product Fos in the posterior thalamus, a brain region associated with the enhancement of responses to mechanical stimulation of the dura by light, and thought to be the basis for migrainous photophobia. Additionally, light induced the phosphorylation of extracellular-related kinase (pERK) in neurons of the central amygdala, an intracellular signal associated with the processing of the aversive aspects of pain. However, light did not activate Fos expression in the spinal trigeminal nucleus caudalis, the primary receptive field for painful stimulation to the head. We conclude that these light-evoked vocalizations and the distinct pattern of brain activation in neonatal mice are consistent with a melanopsin-dependent neural pathway involved in processing light as an aversive but not acutely painful stimulus.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Light Evokes Melanopsin-Dependent Vocalization and Neural Activation Associated with Aversive Experience in Neonatal Mice

et al. 2012

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“…In 3 weeks after the operation the influence of hemantane on the rat's olfactory impairment induced by LPS injection was estimated using a modification of the method described by Lemasson and coworkers [34]. The rat was placed on a square arena (measuring 60 × 60 cm with the wall height of 30 cm) divided into 9 equal squares.…”

Section: Methodsmentioning

confidence: 99%

The activity of antiparkinsonian drug hemantane in models of peripheral inflammation and lipopolysaccharide-induced neuroinflammation

Ivanova¹,

Kapitsa²,

Sukhorukova³

et al. 2013

APD

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A large body of literature supports the idea that inflammation exacerbates neurodegenerative pathology. This idea is also supported by the fact that intracerebral or intraperitoneal injection of lipopolysaccharide (LPS) induces symptoms of Parkinson's disease in rats. The aim of this study is to evaluate the anti-inflammatory effects of the novel antiparkinsonian drug hemantane (N-2(adamantyl)hexamethylenimine hydrochloride), which is currently undergoing clinical trials, in models of peripheral inflammation and neuroinflammation and to investigate its ulcerogenic action, which is a common side effect of nonselective nonsteroidal anti-inflammatory drugs. Acetic acid-induced peritonitis in mice was used as a model of peripheral inflammation. Effect on the stomach was investigated in rats were deprived of food for 16 hours and then were treated with 0.2 LD 50 of hemantane or the comparator drug diclofenac sodium per os. Injection of LPS in the left substantia nigra pars compacta in rats was chosen as a model of neuroinflammation. LPS-induced body weight loss, forelimb akinesia and behavioral changes caused by irritating odor were registered in rats. Hemantane in the dosage range of 10 -40 mg/kg demonstrates anti-inflammatory activity and significantly decreases the intensity of exudative reaction in a model of acetic acid-induced peritonitis in mice. Additionally, at the dose of 0.2 LD 50 orally it did not damage the gastric mucosa of rats. In a model of neuroinflammation induced by a unilateral injection of LPS, hemantane (10 mg/kg) prevents weight loss, development of forepaw akinesia contralateral to the operation, and smell disturbance in rats. Effectiveness of hemantane in the animal models of peripheral inflammation and neuroinflammation make it possible to suggest a new application of hemantane as a safe anti-inflammatory drug.

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“…(Teicher and Blass, 1976 Yang and Crawley, 2009), there is a serious limitation in exploring olfaction in very young rodents due to their limited behavioral repertoire. Nevertheless, a suitable test to address odor perception in rodent pups has been designed based on the recording of ultrasonic isolation calls (Hofer and Shair, 1991;Hofer et al, 2002;Lemasson et al, 2005;Lazarini et al, 2018). Young pups, while isolated from the mother and littermates and placed in low ambient temperature produce ultrasonic vocalizations (USV) at a high rate (Smith and Sales, 1980;Branchi et al, 1998;Castellucci et al, 2018), that promote maternal behavior such as searching for pups, retrieving and licking of pups (Noirot, 1974 Lahlou et al, 1992;Brunelli et al, 1994).…”

mentioning

confidence: 99%

Assessing Olfaction Using Ultrasonic Vocalization Recordings in Mouse Pups with a Sono-olfactometer

Wagner¹,

Lledo²,

Lazarini³

2019

BIO-PROTOCOL

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Olfaction is the first sensory modality to develop during fetal life in mammals, and plays a key role in the various behaviors of neonates such as feeding and social interaction. Odorant cues (i.e., mother or predator scents) can trigger potentiation or inhibition of ultrasonic vocalizations (USV) emitted by pups following their isolation. Here, we report how USV are inhibited by olfactory cues using a sonoolfactometer that has been designed to quantify precisely olfaction in pups congenitally infected by cytomegalovirus. This olfactory-driven behavioral test assesses the USV emitted in presence of unfamiliar odorants such as citral scent or adult male mouse scent. We measure the number of USV emitted as an index of odorant detection during the three periods of the 5-min isolation time of the pup into the sono-olfactometer: first period without any odorant, second period with odorant exposure and last period with exhaust odorant. This protocol can be easily used to reveal olfactory deficits in pups with altered olfactory system due to toxic lesions or infectious diseases.

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Use of ultrasonic vocalizations to assess olfactory detection in mouse pups treated with 3-methylindole

Cited by 24 publications

References 70 publications

Light Evokes Melanopsin-Dependent Vocalization and Neural Activation Associated with Aversive Experience in Neonatal Mice

Light Evokes Melanopsin-Dependent Vocalization and Neural Activation Associated with Aversive Experience in Neonatal Mice

The activity of antiparkinsonian drug hemantane in models of peripheral inflammation and lipopolysaccharide-induced neuroinflammation

Assessing Olfaction Using Ultrasonic Vocalization Recordings in Mouse Pups with a Sono-olfactometer

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