Use of two short-term tests to evaluate the genotoxicity of river water treated with different concentration/extraction procedures

Vargas, Vera Maria Ferrão; Guidobono, Regis Rolim; Jordão, Cleusa; Henriques, João Antônio Pêgas

doi:10.1016/0165-1218(95)90060-8

Cited by 68 publications

(35 citation statements)

References 17 publications

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“…Even though overall pollution parameters are declining in many rivers of Europe and actual contaminant concentrations are below concentrations with acute e!ects (Den Besten et al, 1995;MuK ller et al, 1993;Vargas et al, 1995), there is still an urgent need for biomonitoring, since the number of manmade chemicals released into the environment is still growing. Furthermore, rising drinking water consumption rates in developed countries will require the direct use of rivers as a source for drinking water at increasing rates.…”

Section: Discussionmentioning

confidence: 98%

Tail Moment versus Tail Length—Application of an In Vitro Version of the Comet Assay in Biomonitoring for Genotoxicity in Native Surface Waters Using Primary Hepatocytes and Gill Cells from Zebrafish (Danio rerio)

Schnurstein

Braunbeck

2001

Ecotoxicology and Environmental Safety

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Section: Discussionmentioning

confidence: 98%

Tail Moment versus Tail Length—Application of an In Vitro Version of the Comet Assay in Biomonitoring for Genotoxicity in Native Surface Waters Using Primary Hepatocytes and Gill Cells from Zebrafish (Danio rerio)

Schnurstein

Braunbeck

2001

Ecotoxicology and Environmental Safety

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“…In further development of the MPIA, it will be helpful to consider the steps used in development of the microscreen assay. Similar work with environmental applications of the microscreen assay has determined that in all cases it is most desirable to use unprocessed environmental samples (Vargas et al, 1995). Any chemical extraction procedure decreases the sensitivity of the assay.…”

Section: Discussionmentioning

confidence: 97%

“…Other types of tests respond to mutagenic agents by different physiological end points. However, the phage induction type of assay appears to be senitive to more varied types of substances probably because it is sensitive to more types of DNA damage (Vargas et al, 1995). For this reason a prophage induction type of assay similar in concept to the microscreen assay was selected for development.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Marine Bacterial Lysogens for Development of a Marine Prophage Induction Assay

McDaniel

Griffin²,

Crespo-Gómez

et al. 2001

Marine Biotechnology

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The demonstrated relationship between carcinogenicity of a chemical compound in mammals and its tendency to cause prophage induction in bacteria provides a method for biologically based carcinogen screening. Because of the need for this type of screening and the abundance of lysogens in the marine environment, 14 isolates were evaluated for the degree of prophage induction in exponentially growing cultures in the presence of a known mutagen (0.5 microg/ml mitomycin C). Assays were performed both in liquid culture and in microtiter plates. Virus-like particles were enumerated by epifluorescence microscopy after staining with SYBR-Gold. Two isolates designated P94-4B3 (identified as Halomonas aquamarina) and P94-4S3 (identified as Pseudomonas aeruginosa) were further evaluated for potential use. Because of the rapid growth, larger size of its virus-like particles, and linear response to increasing dose of mitomycin C, the P. aeruginosa st. P94-4S3 was determined a better candidate for the marine prophage induction assay (MPIA). The Pseudomonas isolate was then used in several experiments for the development and optimization of the MPIA procedure. Initial screenings of the MPIA were also performed with selected environmental pollutants.

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“…Methyl methane sulphonate and sodium azide served as positive controls. The criterion used to classify the results as positive was similar to that of Vargas et al, [19]: number of revertants double the spontaneous yields accompanied by a reproducible dose-response curve.…”

Section: Salmonella Mutagenicity Testmentioning

confidence: 99%

Mutagenicity of Wastewater Extracts from Pulp and Paper Industry

Aiman¹,

Malik²

2017

JEP

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Wastewater samples were collected from pulp and paper mill located in Kashipur (India) and were extracted using dichloromethane (DCM), chloroform and hexane solvents (all were HPLC-grade). The extracts were assayed for their mutagenic potential using Ames Salmonella mutagenicity assay. TA98 strain was found to be the most responsive, in terms of induction factor (Mi) and slope (m) of the initial linear dose-response curve as determined by linear regression analysis up to the increasing doses indicating the presence of frame shift mutagens in the test samples. Mutagenicity of different extracts is arranged as follows: dichloromethane extracted water samples > hexane extracted water samples > chloroform extracted water samples. Hexane extract exhibited maximum mutagenic index of 13.0 and induction factor (Mi) 2.48 with TA98. The order of responsiveness based on the mutagenic index and induction factor for the test samples was in the following order: TA98 > TA97a > TA100 > TA102 > TA104. Our findings suggest that TA97a, TA98, TA100, TA102, TA104 were sensitive towards the wastewater extracts and showed considerable mutagenicity.

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Use of two short-term tests to evaluate the genotoxicity of river water treated with different concentration/extraction procedures

Cited by 68 publications

References 17 publications

Tail Moment versus Tail Length—Application of an In Vitro Version of the Comet Assay in Biomonitoring for Genotoxicity in Native Surface Waters Using Primary Hepatocytes and Gill Cells from Zebrafish (Danio rerio)

Tail Moment versus Tail Length—Application of an In Vitro Version of the Comet Assay in Biomonitoring for Genotoxicity in Native Surface Waters Using Primary Hepatocytes and Gill Cells from Zebrafish (Danio rerio)

Evaluation of Marine Bacterial Lysogens for Development of a Marine Prophage Induction Assay

Mutagenicity of Wastewater Extracts from Pulp and Paper Industry

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