Use of two‐dimensional gel electrophoresis to characterize protein synthesis during neuronal death in cerebellar culture

Charriaut‐Marlangue, Christiane; Dessi, Frédéric; Ben-Ari, Yehezkel

doi:10.1002/elps.1150171117

Cited by 5 publications

(3 citation statements)

References 39 publications

(8 reference statements)

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“…,095. Buonocore et trophoresis (Amess and Tolkovsky 1^^^' *^" , al.. 1999;Charriaut-Marlangue et al 1996. Jenk n et^al 2002-Leski and Steward.…”

Section: Discussionmentioning

confidence: 99%

Biochemical Markers of Brain Injury: An Integrated Proteomics-Based Approach

Hayes¹

2005

PsycEXTRA Dataset

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Near-completion of the Human Genome Project has stimulated scientists to begin looking for the next step in unraveling normal and abnormal functions within biological systems. Consequently, there is new focus on the role of proteins in these processes. Proteomics is a burgeoning field that may provide a valuable approach to evaluate the post-trauihatic central nervous system (CNS). Although we cannot provide a comprehensive assessment of all methods for protein analysis, this report summarizes some of the newer proteomic technologies that have propelled this field into the limelight and that are available to most researchers in neurotrauma. Three technical approaches (two-dimensional gel electrophoresis, direct analysis by mass spectrometry, including two-dimensional chromatography coupled to mass spectrometry and isotope coded affinity tags, and antibody technologies) are reviewed, and their advantages and disadvantages presented. A discussion of proteomic technology in the context of brain and spinal cord trauma follows, addressing current and future challenges. Proteomics will likely be very useful for developing diagnostic predictors after CNS injury and for mapping changes in proteins after injury in order to identify new therapeutic targets. Neurotrauma results in complex alterations to the biological systems within the nervous system, and these changes evolve over time. Exploration of the "new nervous system" that follows injury will require methods that can both fully assess and simplify this complexity.

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“…,095. Buonocore et trophoresis (Amess and Tolkovsky 1^^^' *^" , al.. 1999;Charriaut-Marlangue et al 1996. Jenk n et^al 2002-Leski and Steward.…”

Section: Discussionmentioning

confidence: 99%

Biochemical Markers of Brain Injury: An Integrated Proteomics-Based Approach

Hayes¹

2005

PsycEXTRA Dataset

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“…Although the study of proteins is not new, the field of "proteomics" is. In the past, several studies of CNS injury have used the high-resolution power of 2D gel electrophoresis (Amess and Tolkovsky, 1995;Buonocore et al, 1999;Charriaut-Marlangue et al, 1996;Jenkins et al 2002;Leski and Steward, 1996). The work of Kirschenbaum and Pulsinelli (1990) performed over a decade ago to analyze differences in phosphorylation patterns in ischemic rat hippocampus, striatum, and neocortex tissues was extremely interesting and forward thinking for its time.…”

Section: Discussionmentioning

confidence: 99%

“…It is possible to see over 1,000 well-resolved proteins on a single gel. While the application of 2D GE is not new to neuroscience research (Amess and Tolkovsky, 1995;Buonocore et al, 1999;Charriaut-Marlangue et al, 1996), the new interest in this method is the ability to directly identify proteins that are differentially expressed by mass spectrometry (MS).…”

Section: Two-dimensional Gel Electrophoresis Followed By Mass Spectromentioning

confidence: 99%

Application of Proteomics Technology to the Field of Neurotrauma

Denslow

Michel²,

Temple³

et al. 2003

Journal of Neurotrauma

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Near-completion of the Human Genome Project has stimulated scientists to begin looking for the next step in unraveling normal and abnormal functions within biological systems. Consequently, there is new focus on the role of proteins in these processes. Proteomics is a burgeoning field that may provide a valuable approach to evaluate the post-traumatic central nervous system (CNS). Although we cannot provide a comprehensive assessment of all methods for protein analysis, this report summarizes some of the newer proteomic technologies that have propelled this field into the limelight and that are available to most researchers in neurotrauma. Three technical approaches (two-dimensional gel electrophoresis, direct analysis by mass spectrometry, including two-dimensional chromatography coupled to mass spectrometry and isotope coded affinity tags, and antibody technologies) are reviewed, and their advantages and disadvantages presented. A discussion of proteomic technology in the context of brain and spinal cord trauma follows, addressing current and future challenges. Proteomics will likely be very useful for developing diagnostic predictors after CNS injury and for mapping changes in proteins after injury in order to identify new therapeutic targets. Neurotrauma results in complex alterations to the biological systems within the nervous system, and these changes evolve over time. Exploration of the "new nervous system" that follows injury will require methods that can both fully assess and simplify this complexity.

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