2023
DOI: 10.1016/j.crfs.2023.100615
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Use of two autochthonous bacteriocinogenic strains as starter cultures in the production of salchichónes, a type of Spanish fermented sausages

J. David García-López,
Federica Barbieri,
Alberto Baños
et al.
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“…For microbial and fungal communities, the 16S rDNA V3-V4 region using the locusspecific polymerase chain reaction (PCR) primers 16S 341F/805R [46] and the internal transcribed spacer (ITS) region using the primer pairs ITS1F/ITS4R [47,48] were amplified with the integration of relevant flow-cell binding domains and unique indices. The libraries obtained were sequenced on a MiSeq Illumina instrument using 300-bp paired-end mode, and the FASTQ sequences generated were analyzed using DADA2 version 1.12.1 [49] in the R 3.5.1 environment.…”
Section: Dna Extraction and Taxonomic Assignmentmentioning
confidence: 99%
“…For microbial and fungal communities, the 16S rDNA V3-V4 region using the locusspecific polymerase chain reaction (PCR) primers 16S 341F/805R [46] and the internal transcribed spacer (ITS) region using the primer pairs ITS1F/ITS4R [47,48] were amplified with the integration of relevant flow-cell binding domains and unique indices. The libraries obtained were sequenced on a MiSeq Illumina instrument using 300-bp paired-end mode, and the FASTQ sequences generated were analyzed using DADA2 version 1.12.1 [49] in the R 3.5.1 environment.…”
Section: Dna Extraction and Taxonomic Assignmentmentioning
confidence: 99%