Use of Tween 80 agar in the study of Moraxella bovis infection in cattle

Mattinson, AD; Cox, PJ

doi:10.1136/vr.111.17.395

Cited by 8 publications

(4 citation statements)

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“…Bacteriology: The strains were plated onto 5 % defibrinated sheep blood agar (Bio Merieux, Lyon) and incubated at 37°C under aerobic atmosphere. After 24 hours, the strains were subcultured on brain heart infusion agar (BHIA) or brain heart infusion broth (BHIB) (Merck) for identification in biochemical tests as specified by h o u (32), MATTINSON and Cox (22), and WILCOX (39), namely producing catalase using ID color test (BioMCrieux), cytochrome oxidase C in 1 YO aqueous solution of tetramethylparaphenylendiamin (SIGMA), acids production from 1 YO of each following sugar: glucose, maltose and lactose in Cystine Trypticase Agar (BioMirieux), reducing nitrates, growth on BHIA under aerobic and anaerobic conditions, growth on McConkey and nutrient agars, growth on mineral medium with acetate, indole and urease production, tributyrin, tween 80, deoxyribonucleic acid hydrolysis and gelatin hydrolysis and milk peptonisation. Serum (Institut Pasteur Production) liquefaction and cellular elongation (5) were also tested.…”

Section: Methodsmentioning

confidence: 99%

Identification of Moraxella‐like Bacteria Isolated from Caprine and Ovine Nasal Flora

Kodjo

Moussa

Borges

et al. 1993

Journal of Veterinary Medicine, Series B

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Summary Twenty four Moraxella related bacterias were isolated from healthy caprine and ovine nasal swabs and were investigated by classic biochemical tests and by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) of whole‐cell proteins in comparison with 9 reference strains. Proteolytic and haemolytic strains were investigated by electron microscopy. The biochemical results clustered field isolates in four groups corresponding to Branhamella and Moraxella species. Proteolytic, haemolytic and fimbriated field isolates showed the same morphological structure and biochemical features as Moraxella bovis. SDS‐PAGE results indicated that DICE coefficient between a field isolate and the corresponding reference strain can be as 62.5%; 41.7% and 36% respectively for the groups 1, 3 and 4. The group 2 showed a similarity percentage over 75 % with the reference strain Moraxella nonliquefaciens. This results indicated that a non proteolytic but haemolytic bacteria, closely related to Moraxella nonliquefaciens was commonly isolated from small ruminants nasal flora. These animals can also be hosts of a subspecies of Moraxella bovis. Résumé Vingt‐quatre souches de bactéries Moraxella‐like ont été isolées de la flore nasale de petits ruminants sains et analysées par les méthodes conventionnelles de bactériologie et par l'analyse du profil électrophorétique de leurs proteines totales en gel d'acrylamide en comparaison avec 9 souches de référence. Les souches protéolytiques et hémolytiques ont fait l'objet d'une étude au microscope électronique. Les résultats biochimiques ont permis de rassembler les isolats en 4 groupes correspondant à des espèces de Branhamella et de Moraxella. Les souches protéolytiques, hémolytiques et fimbriées ont présenté les mêmes caractères morphologiques et biochimiques que Moraxella bovis. L'analyse des profils électrophorétiques des isolats par rapport aux souches de référence correspondantes ont donné des coefficients de Dice de 62.5%, 41.7% et 36% respectivement pour les groupes 1, 3 et 4. Le coefficient de Dice du groupe 4 par rapport à Moraxella nonliquefaciens était supérieur à 75 %. Ces résultats ont confirmé l'existence d'une espèce de Moraxella bovis et la présence de souches hémolytiques et non protéolytiques de Moraxella nonliquefaciens dans la flore nasale commensale de petits ruminants.

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Section: Methodsmentioning

confidence: 99%

Identification of Moraxella‐like Bacteria Isolated from Caprine and Ovine Nasal Flora

Kodjo

Moussa

Borges

et al. 1993

Journal of Veterinary Medicine, Series B

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“…Isolation Swabs were streaked on 10% sheep blood agar plates 16 and tween 80 agar plates, 17 which were then incubated at 37 °C for 48 to72 h under aerobic conditions.…”

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confidence: 99%

“…Identification After incubation, isolated strains were identified on the basis of the following criteria: colony characteristics; Gram‐staining reaction and morphology; cellular elongation in the presence of penicillin, growth characteristics in trypticase soy broth (TSB); motility; oxidase; catalase; growth on MacConkey agar; growth at 20 °C; tolerance to 5% NaCl; oxidation‐fermentation (O‐F); carbohydrate (glucose, lactose, arabinose, xylose, mannose) fermentation; indole and hydrogen sulfide production; nitrate reduction; urease; tween 80 and casein hydrolysis; coagulated serum; and gelatine liquefaction 15–18 …”

mentioning

confidence: 99%