Use of tween 20 as a substrate for assay of lipase activity in soils

Sakai, Yasuo; Hayatsu, Masahito; Hayano, Kimitoshi

doi:10.1080/00380768.2002.10409263

Cited by 15 publications

(7 citation statements)

References 10 publications

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“…Tween-20, a detergent that has fatty acids (C 12 ) with medium chain length, has been reported as a potential substrate for the assay of soil lipase activity [ 23 , 25 , 26 ]. The use of Tweens has been criticized due to the possibility of Tweens being hydrolyzed by esterases, thus resulting in false-positive results in the lipase-screening assay [ 27 , 28 ].…”

Section: Resultsmentioning

confidence: 99%

Lipase-SecretingBacillusSpecies in an Oil-Contaminated Habitat: Promising Strains to Alleviate Oil Pollution

Lee

Karbul

Citartan

et al. 2015

BioMed Research International

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Lipases are of great interest for different industrial applications due to their diversity and versatility. Among different lipases, microbial lipases are preferable due to their broad substrate specificity, and higher stability with lower production costs compared to the lipases from plants and animals. In the past, a vast number of bacterial species have been reported as potential lipases producers. In this study, the lipases-producing bacterial species were isolated from an oil spillage area in the conventional night market. Isolated species were identified as Bacillus species by biochemical tests which indicate their predominant establishment, and further screened on the agar solid surfaces using lipid and gelatin as the substrates. Out of the ten strains tested, four potential strains were subjected to comparison analysis of the lipolytic versus proteolytic activities. Strain 10 exhibited the highest lipolytic and proteolytic activity. In all the strains, the proteolytic activity is higher than the lipolytic activity except for strain 8, suggesting the possibility for substrate-based extracellular gene induction. The simultaneous secretion of both the lipase and protease is a mean of survival. The isolated bacterial species which harbour both lipase and protease enzymes could render potential industrial-based applications and solve environmental issues.

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Section: Resultsmentioning

confidence: 99%

Lipase-SecretingBacillusSpecies in an Oil-Contaminated Habitat: Promising Strains to Alleviate Oil Pollution

Lee

Karbul

Citartan

et al. 2015

BioMed Research International

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“…[27][28][29][30][31][32][33] The Tween family (Tween 20, 40, 60, 80 and so on) are amphipathic, nonionic surfactants composed of fatty acid esters of polyoxyethylene sorbitan, which have been used as substrates for lipase recognition. [34][35][36][37][38] Among them, Tween 80 differs in molecular structure; the fatty acid ester side chain is unsaturated, containing a double bond. It has been reported that Tween 80 took the role of a reductant in the in situ synthesis of AuNPs.…”

Section: Introductionmentioning

confidence: 99%

A plasmonic nanosensor for lipase activity based on enzyme-controlled gold nanoparticles growth in situ

et al. 2015

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A plasmonic nanosensor for lipase activity was developed based on one-pot nanoparticle growth. Tween 80 was selected not only as the substrate for lipase recognition but also as the reducing and stabilizing agent for the sensor fabrication. The different molecular groups in Tween 80 could have different roles in the fabrication procedure; the H2O2 produced by the autoxidation of the ethylene oxide subunits in Tween 80 could reduce the AuCl4(-) ions to Au atoms, meanwhile, the lipase could hydrolyze its carboxyl ester bond, which could, in turn, control the rate of nucleation of the gold nanoparticles (AuNPs) and tailor the localized surface plasmon resonance (LSPR) of the AuNP transducers. The color changes, which depend on the absence or presence of the lipase, could be used to sense the lipase activity. A linear response ranging from 0.025 to 4 mg mL(-1) and a detection limit of the lipase as low as 3.47 μg mL(-1) were achieved. This strategy circumvents the problems encountered by general enzyme assays that require sophisticated instruments and complicated assembling steps. The methodology can benefit the assays of heterogeneous-catalyzed enzymes.

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“…Various methods are developed for the determination of lipase activity or in turn the final products, that is fatty acid estimations. Currently, the commonly available assays include titrimetry (Pokrana 1964), turbidometry (Sakai 2002), fluorimetry (Cooper 1981) and colourimetry (Margesin 2002). But, due to low sensitivity and encountering with titrimetry , is the restricted range of pH values that can be investigated (Beisson et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Biodiesel Production from Oleaginous Fungi

Ravikumar¹,

Dakshayini²,

Girisha³

2012

Int J Life Sci

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Biodiesel involves the mixture of fatty acyl methyl/ethyl esters, produced from transesterification neutral lipids and if the origin of the source is from oleaginous micro organisms, then it is termed as micro diesel. In the present work, aiming to exploit fungi for biodiesel production, 12 fungal isolates were screened for lipid content by Sudan Black B staining method. Among 12 isolates, lipid rich five species viz, Mortierella alpina , M.ramanianna, M.vinancea, M.hyalina and M.verticella have been taken for fatty acids analysis by spectrophotometry, which revealed that the amount of free fatty acids were ranged from highest in M.alpina 35 ?moles of Oleic acid , 25 ?moles of Palmitic acid and 14 ?moles of Myristic acids to lower as much as 21 ?moles of Oleic acid , 18 ?moles of Palmitic acid and 16 ?moles of Myristic acids respectively in M.ramanianna. DOI: http://dx.doi.org/10.3126/ijls.v6i1.5721

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Use of tween 20 as a substrate for assay of lipase activity in soils

Cited by 15 publications

References 10 publications

Lipase-SecretingBacillusSpecies in an Oil-Contaminated Habitat: Promising Strains to Alleviate Oil Pollution

Lipase-SecretingBacillusSpecies in an Oil-Contaminated Habitat: Promising Strains to Alleviate Oil Pollution

A plasmonic nanosensor for lipase activity based on enzyme-controlled gold nanoparticles growth in situ

Biodiesel Production from Oleaginous Fungi

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