Use of tubularized peritoneal free grafts as urethral substitutes in the rabbit

Shaul, Donald B.; Xie, Hui; Diaz, Jessica; Mahnovski, Vladimir; Hardy, Brian

doi:10.1016/s0022-3468(96)90002-0

Cited by 25 publications

(17 citation statements)

References 10 publications

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“…Up to 6 months after the surgery, no urethral stricture occurred. In previous studies, mesothelial cells have been shown to convert into urothelium when placed in the urinary tract [12,13,17]. We observed a similar phenomenon.…”

Section: Discussionsupporting

confidence: 87%

“…They are also easy to be cultured in vitro and the commonly used DMEM medium could satisfy their nutritional requirement [10,11]. Mesothelium-lined grafts including peritoneum [12,13] and tunica vaginalis [14,15] have already been attempted in urethral reconstruction with good results. In our previous study, we successfully used a kind of mesothelium-lined autologous tubular tissue as urethral graft [16].…”

Section: Discussionmentioning

confidence: 99%

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Tubularized Urethral Replacement Using Tissue-Engineered Peritoneum-Like Tissue in a Rabbit Model

Xia²,

Zhang³

et al. 2012

Urol Int

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Introduction: This study aims to investigate whether mesothelial cells could function as seed cells to construct tissue-engineered peritoneum-like tissue for urethral reconstruction in a rabbit model. Materials and Methods: Bladder acellular matrices were prepared and trimmed to 1.5 × 1 cm. Nine male rabbits underwent omentum biopsy and autologous mesothelial cells were isolated. After in vitro expansion, the cells were seeded onto the matrices and incubated for 7 days. In 18 rabbits, a pendulous urethral segment 1.5 cm long was totally excised and urethroplasty was performed with tubularized matrices seeded with cells in 9 animals and without cells in 9 as controls. Urethrography was performed at 1, 2 and 6 months postoperatively. Meanwhile, the neourethras were harvested and analyzed grossly and histologically. Results: Histological analysis of the cell-seeded grafts revealed a loose collagen matrix covered with a single layer of mesothelim. Serial urethrography indicated a wide urethral caliber without stricture formation in animals implanted with cell-seeded matrices, while all animals of the control group developed stricture. Histological analysis of the implanted cell-seeded matrices demonstrated a normal urethral architecture by 1 month, composed of multilayers of urothelium surrounded by smooth muscle bundles, which became increasingly organized with time. By 6 months, the neourethra could be hardly distinguished from native urethra both grossly and histologically. Conclusions: Autologous mesothelial cells could be successfully used as seed cells for tubularized urethral reconstruction in male rabbits.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Tubularized Urethral Replacement Using Tissue-Engineered Peritoneum-Like Tissue in a Rabbit Model

Xia²,

Zhang³

et al. 2012

Urol Int

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“…Several kinds of tissue have been used to correct complex urethral lesions: vein (1), vermiform appendix (2), uterine tube (3), ureter (4), skin (5), bladder mucosa (6), buccal mucosa (7), dura mater (8), peritoneum (9), tunica vaginalis (10), and preputial island flap (11). The best results have been achieved with preputial island flaps in onestage surgeries for the construction of neourethras (12,13).…”

Section: Introductionmentioning

confidence: 99%

Urethroplasty using a bovine pericardium graft: an experimental study using normal urethras from dogs

Lara

Lucon

Arap

2004

Braz J Med Biol Res

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The use of bovine pericardium as a urethral patch to substitute a ventral segment of canine urethras was studied. Healing, epithelial growth, urethral permeability, fistulas, and calcification were analyzed. Thirty male mongrel dogs of medium and large size underwent resection of a ventral segment of the medial urethra measuring 2.0 x 0.5 cm, which was replaced with a bovine pericardium graft, treated with buffered glutaraldehyde and preserved in formaldehyde. Two running sutures of polygalactin 5-0 were applied, one on each side of the patch. The corpus spongiosum was closed with uninterrupted suture and the skin with interrupted suture of polygalactin 5-0. Six months later, the animals were examined and sacrificed under anesthesia. Retrograde urethrograms showed that the urethral healing was complete in six of the 30 animals, without stenosis, fistulas or dilations. Microscopic examination showed complete epithelization of these six urethras. The remaining 24 animals presented urethrocutaneous fistulas without stenosis, demonstrated by urethral catheterism using a 10-Fr plastic catheter. These data show that a successful urethral reconstruction of the penile urethra was possible in only 20% of the operated animals. Infection and leakage may be the cause of the urethrocutaneous fistulas present in 80% of cases. Further studies are necessary to determine whether such fistulas are avoidable. If they are, the bovine pericardium may well be an option in the treatment of urethral lesions in dogs.

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“…Many types of biological tissues have been used as full-thickness skin grafts [2] and vesical [3] or buccal mucosa grafts [4]. In previous studies, ureter [5], artery [6], vein [7], appendix tissue [8] and, more recently, tunica vaginalis [9], intestinal submucosa [10] and peritoneum [11] have also been used in experimental models or in clinical applications. Romagnoli et al [12] investigated grafts of cultured squamous urethral epithelium in hypospadiac children.…”

Section: Introductionmentioning

confidence: 99%

Patch Urethroplasty Using a Fascia lata Autograft in Male Rabbits

Campodonico

Michelazzi

Ognio³

et al. 2003

Urol Int

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Introduction: Urethral reconstruction is difficult when the genital skin is not available for surgery. We evaluate the feasibility of using the autologous fascia lata as a graft for urethral repair. Materials and Methods: 10 male rabbits underwent urethroplasty after creation of a ventral urethral defect. The defect was repaired using a graft harvested from the fascia lata. The animals were divided into three groups and sacrificed at 2, 4 and 12 weeks postoperatively. Radiologic control was performed after 10–12 days and before sacrifice. Results: In the 10 rabbits subjected to surgery, no case of death or wound infection was observed. During urethrography, a fistula was observed in 2 animals. In the remainder (n = 8), histological analysis showed the preservation of the original laminar structure without graft shrinkage or fibrosis. On the luminal side of the patch, a new line of urothelium appeared in the 2nd week after surgery. After 3 months, the new epithelium was multilayered and the graft edges were not detectable. No voiding dysfunction was detectable in 8 rabbits. Conclusions: Our study suggests the feasibility of using the autologous fascia lata for urethral patch repair.

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Use of tubularized peritoneal free grafts as urethral substitutes in the rabbit

Cited by 25 publications

References 10 publications

Tubularized Urethral Replacement Using Tissue-Engineered Peritoneum-Like Tissue in a Rabbit Model

Tubularized Urethral Replacement Using Tissue-Engineered Peritoneum-Like Tissue in a Rabbit Model

Urethroplasty using a bovine pericardium graft: an experimental study using normal urethras from dogs

Patch Urethroplasty Using a Fascia lata Autograft in Male Rabbits

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