Use of Triton WR-1339 in cytochemical and biochemical characterization of phytolysosomes

Coulomb, Claude; Coulomb, P.; Michel, G.

doi:10.1007/bf01041213

Cited by 9 publications

(5 citation statements)

References 11 publications

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“…23 in ref. 60) and in the root meristem of Hordeum sativum, 50,51 Cucurbita pepo, 52,[61][62][63][64][65] Schorzonera hispanica, 66 and Euphorbia characias. 55,56 These structures have also been observed prior to death in tapetum cells of Lobivia rauschii (Fig.…”

Section: Macroautophagymentioning

confidence: 99%

Ultrastructure of autophagy in plant cells

2013

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Section: Macroautophagymentioning

confidence: 99%

Ultrastructure of autophagy in plant cells

2013

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“…A vacuole is then formed. Coulomb et al 17 show the formation of a similar structure, consisting of two relatively long membranes that are positioned close together. The two membranes together encircle a portion of the cytoplasm and sequester it.…”

Section: Discussionmentioning

confidence: 99%

Amorphous areas in the cytoplasm ofDendrobiumtepal cells

2013

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In Dendrobium flowers some tepal mesophyll cells showed cytoplasmic areas devoid of large organelles. Such amorphous areas comprised up to about 40% of the cross-section of a cell. The areas were not bound by a membrane. The origin of these areas is not known. We show data suggesting that they can be formed from vesicle-like organelles. The data imply that these organelles and other material become degraded inside the cytoplasm. This can be regarded as a form of autophagy. The amorphous areas became surrounded by small vacuoles, vesicles or double membranes. These seemed to merge and thereby sequester the areas. Degradation of the amorphous areas therefore seemed to involve macroautophagy.

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“…Ces formations pourraient alors Etre assimilCes ii de vCritables vacuoles hCtCrophagiques qui, dans les stades avancCs de leur dCveloppement, fusionneraient avec la grande vacuole centrale de la cellule, comme le suggkrent certaines micrographies. La dynamique membranaire mise en oeuvre au cours de leur Cdification rappelle celle dCcrite pour la constitution des enveloppes de skquestration par confluence de petites vacuoles d'origine rCticulaire ou golgienne confkrant ii la vacuole un Cquipement hydrolasiquede~dCpart qui conditionne son activitC lytique (Coulomb et al 1982). Le flux membranaire se fait dans le sens endoplasmique exoplasmique, le matCriau is016 Ctant ici en position extracellulai~e.…”

Section: Discussionunclassified

Caractérisation ultrastructurale d'une résistance induite par un éliciteur d'origine fongique, chez un cultivar sensible de Piment

Coulomb¹,

Coulomb²,

Saimmaime³

et al. 1990

Can. J. Bot.

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R e~u le 29 novembre 1988 COULOMB, C., COULOMB, P. J., SAIMMAIME, I., LIZZI, Y., et POLIAN, C. 1990. CaractCrisation utlrastructurale d'une rCsistance induite par un Cliciteur d'origine fongique chez un cultivar sensible de Piment. Can. J. Bot. 68 : 381-390.Lorsque le systkme racinaire de jeunes plantules de Piment (Capsicum annuum) d'un cultivar sensible est immergC dans une solution Clicitrice issue du milieu de culture du Trichoderma album, une forte induction de rksistance est constatie dans les feuilles infectCes par le Phytophthora capsici. Dans les tissus envahis par le champignon, les cellules h6tes prksentent un aspect normal et dCveloppent des formations pariktales qui ressemblent aux papilles typiques, mais s'en distinguent par la prksence d'une substance d'origine cytoplasmique, laquelle est phagocytCe et dCgradCe conferant B ces enclaves une fonction lytique et une dynamique particulikre. L'intemention du rkticulum endoplasmique et des peroxysomes B un Cventuel processus de detoxification est CvoquCe. La resistance qui apparait sous l'effet de 1'Cliciteur semble programmCe par la mise en place d'un Ctat de reaction gCnCrateur d'une rCponse de dCfense. COULOMB, C., COULOMB, P. J., SAIMMAIME, I., LIZZI, Y., and POLIAN, C. 1990. Caractirisation utlrastructurale d'une resistance induite par un Cliciteur d'origine fongique chez un cultivar sensible de Piment. Can. J. Bot. 68: 381-390.Elicitation of a sensitive sweet pepper (Capsicum annuum) cultivar roots by immersion in the culture medium of Trichoderma album induces resistance in leaves infected by Phytophthora capsici. In infected tissues, host cells are intact and develop wall formations that look like typical wall appositions, but differ from them by the presence of a substance of cytoplasmic origin, which is phagocytized and degraded, confemng a lytic function and unusual dynamics to these structures. The involvement of the endoplasmic reticulum and peroxysomes in eventual detoxification processes is discussed. The resistance induced by this elicitor seems to be programmed by the establishment of a reactive physiological state, which produces the defense response.

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Use of Triton WR-1339 in cytochemical and biochemical characterization of phytolysosomes

Cited by 9 publications

References 11 publications

Ultrastructure of autophagy in plant cells

Ultrastructure of autophagy in plant cells

Amorphous areas in the cytoplasm ofDendrobiumtepal cells

Caractérisation ultrastructurale d'une résistance induite par un éliciteur d'origine fongique, chez un cultivar sensible de Piment

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