Use of transcriptomics in hazard identification and next generation risk assessment: A case study with clothianidin

Sprenger, Heike; Kreuzer, Katrin; Alarcan, Jimmy; Herrmann, Kristin; Buchmüller, Julia; Marx‐Stoelting, Philip; Braeuning, Albert

doi:10.1016/j.fct.2022.113212

Cited by 8 publications

(3 citation statements)

References 49 publications

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“…Transcriptomic changes in model systems can be used to discover biomarkers of disease and in risk assessment, and these data can provide an understanding of the relationship between molecular pathways and pathology of disease. This information can be used in risk assessment for linking pathologies and molecular pathways ( Merrick, 2019 ; Szabo and Devlin, 2019 ; Sprenger et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiling after exposure to a chemical carcinogen, Pentabrominated Diphenyl Ether, at different life stages

Shockley

Dunnick

2023

Front. Toxicol.

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Exposure to environmental hazards occurs at different stages of our lifetime–infant, child, adult. This study integrates recently published toxicogenomics data to examine how exposure to a known rat chemical carcinogen (pentabrominated diphenyl ether (PBDE)) upregulated liver transcriptomic changes at different life cycle stages (PND 4, PND 22, adult). We found that at all three life cycle stages PBDE exposure induced hepatocellular transcriptomic changes in disease pathways including cancer, metabolic, membrane function, and Nrf2 antioxidant pathways, pathways all characteristics of chemical carcinogens. In addition, in the adult rat after a 5-day exposure to the chemical carcinogen, there was upregulation of members of the Ras oncogenic pathway, a specific pathway found to be activated in the PBDE-induced tumors in rats in a previous hazard identification cancer study. The findings of liver transcript changes characteristic of carcinogenic activity after early life exposures and after short-term adult exposures provides data to support the use of transcriptomic data to predict the apical cancer endpoints in model studies. Using data from gene expression profiling studies after neonatal, young, or adult short-term chemical exposure helps to meet the 21st century toxicology goal of developing study designs to reduce, refine, and replace the use of traditional 2-year rodent cancer studies to provide hazard identification information. The studies reported here find that key transcripts associated with carcinogenesis were elevated in neonate (PND 4), young (PND 22) and adult animals after short-term exposure to PBDE, a known experimental chemical carcinogen in model systems.

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Section: Discussionmentioning

confidence: 99%

Gene expression profiling after exposure to a chemical carcinogen, Pentabrominated Diphenyl Ether, at different life stages

Shockley

Dunnick

2023

Front. Toxicol.

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“…iv. γH2AX assay The γH2AX assay was performed according to Sprenger et al 51 Cells were treated for 24 h with different concentrations of SDA. Doxorubicin (1 μM) was used as positive control.…”

Section: Cell Viability Assaymentioning

confidence: 99%

Towards Increased Crop Yields with Molecular Heater Technology

Stavros,

Woolley,

Rodrigues

et al. 2023

Preprint

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Food security is fast becoming one of the most pressing issues of the 21st century. With increasing climate instability, crop growth is now routinely affected by adverse weather patterns, which can result in reduced crop yields and even crop death. We are developing an environmentally responsible molecular heater to help boost plant growth, to expand the geographic regions suitable for such growth and to protect plants against cold weather. Our molecular heater, sinapic diacid (SDA, 2-(4-hydroxy-3,5-dimethoxybenzylidene)malonic acid), acts as a light-to-heat agent, converting solar energy into heat energy delivered to the plant. We have designed and optimised a sustainable chemical synthesis of SDA, characterised its photophysical properties using a combination of steady-state and ultrafast laser spectroscopy techniques and complementary computational studies, assessed its light-to-heat energy transfer performance to plant leaves in vivo, and explored key environmental and toxicological endpoints through in silico studies. Our spot-test results demonstrate that adsorbed SDA efficiently converts solar energy to heat energy, increasing the local plant leaf temperature by ~ 0.2 °C after sustained irradiation, with no alerts to environmental fate and human toxicity, and with impressive resilience to prolonged solar irradiation. Our studies reveal the hitherto untapped potential of molecular heaters such as SDA for helping to boost plant growth and protect plants against climate instability, with transformative potential towards food security.

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“…10 Studies have demonstrated the signicant effects of CLD on birds, sh and mice. 11 In addition to these, bees. [12][13][14] In general, these negative effects presented were gene mutations, carcinogenic alterations, disorientation and, principally, death of nontarget insects such as honeybees.…”

Section: Introductionmentioning

confidence: 99%

Development of an electrochemical sensor based on ternary oxide SiO₂/Al₂O₃/SnO₂ modified with carbon black for direct determination of clothianidin in environmental and food samples

et al. 2023

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Use of transcriptomics in hazard identification and next generation risk assessment: A case study with clothianidin

Cited by 8 publications

References 49 publications

Gene expression profiling after exposure to a chemical carcinogen, Pentabrominated Diphenyl Ether, at different life stages

Gene expression profiling after exposure to a chemical carcinogen, Pentabrominated Diphenyl Ether, at different life stages

Towards Increased Crop Yields with Molecular Heater Technology

Development of an electrochemical sensor based on ternary oxide SiO₂/Al₂O₃/SnO₂ modified with carbon black for direct determination of clothianidin in environmental and food samples

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Use of transcriptomics in hazard identification and next generation risk assessment: A case study with clothianidin

Cited by 8 publications

References 49 publications

Gene expression profiling after exposure to a chemical carcinogen, Pentabrominated Diphenyl Ether, at different life stages

Gene expression profiling after exposure to a chemical carcinogen, Pentabrominated Diphenyl Ether, at different life stages

Towards Increased Crop Yields with Molecular Heater Technology

Development of an electrochemical sensor based on ternary oxide SiO2/Al2O3/SnO2 modified with carbon black for direct determination of clothianidin in environmental and food samples

Contact Info

Product

Resources

About

Development of an electrochemical sensor based on ternary oxide SiO₂/Al₂O₃/SnO₂ modified with carbon black for direct determination of clothianidin in environmental and food samples