Use of toxicogenomics to predict the potential toxic effect of Benzo(a)pyrene on zebrafish embryos: Ocular developmental toxicity

Huang, Lixing; Zuo, Zhenghong; Zhang, Youyu; Wu, Peng; Lin, Jackie Jin; Wang, Chonggang

doi:10.1016/j.chemosphere.2014.02.078

Cited by 54 publications

(25 citation statements)

References 35 publications

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“…MS-222 (Tricaine methane sulfonate) is used extensively as an anesthetic in fish studies, however at the concentrations typically used (100–200 ppm or mg/L), it acts as a profound cardiodepressant [ 20 ] and can seriously confound the results. In this study a combination of 45 ppm of pH-adjusted MS-222 (Sigma-Aldrich, Oakville, ON) and 45 ppm isoflurane (Sigma-Aldrich, Oakville, ON) was used for zebrafish to allow anaesthesia time to be extended with less consequence to cardiac function in comparison to MS-222 alone [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

Functional Assessment of Cardiac Responses of Adult Zebrafish (Danio rerio) to Acute and Chronic Temperature Change Using High-Resolution Echocardiography

et al. 2016

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The zebrafish (Danio rerio) is an important organism as a model for understanding vertebrate cardiovascular development. However, little is known about adult ZF cardiac function and how contractile function changes to cope with fluctuations in ambient temperature. The goals of this study were to: 1) determine if high resolution echocardiography (HRE) in the presence of reduced cardiodepressant anesthetics could be used to accurately investigate the structural and functional properties of the ZF heart and 2) if the effect of ambient temperature changes both acutely and chronically could be determined non-invasively using HRE in vivo. Heart rate (HR) appears to be the critical factor in modifying cardiac output (CO) with ambient temperature fluctuation as it increases from 78 ± 5.9 bpm at 18°C to 162 ± 9.7 bpm at 28°C regardless of acclimation state (cold acclimated CA– 18°C; warm acclimated WA– 28°C). Stroke volume (SV) is highest when the ambient temperature matches the acclimation temperature, though this difference did not constitute a significant effect (CA 1.17 ± 0.15 μL at 18°C vs 1.06 ± 0.14 μl at 28°C; WA 1.10 ± 0.13 μL at 18°C vs 1.12 ± 0.12 μl at 28°C). The isovolumetric contraction time (IVCT) was significantly shorter in CA fish at 18°C. The CA group showed improved systolic function at 18°C in comparison to the WA group with significant increases in both ejection fraction and fractional shortening and decreases in IVCT. The decreased early peak (E) velocity and early peak velocity / atrial peak velocity (E/A) ratio in the CA group are likely associated with increased reliance on atrial contraction for ventricular filling.

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Section: Methodsmentioning

confidence: 99%

Functional Assessment of Cardiac Responses of Adult Zebrafish (Danio rerio) to Acute and Chronic Temperature Change Using High-Resolution Echocardiography

et al. 2016

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“…Ren et al [ 84 ] demonstrated that BaP (≥1 μM) can cause significant malformation and mortality in developing zebrafish embryos. Also, Huang et al [ 85 ], with the help of microarray analysis, demonstrated that BaP was capable of inducing visual system developmental defects and dysfunction by perturbing photoreceptor development correlated genes. Other authors, including Kazeto et al [ 82 ] found upregulation in cyp19a2 gene after exposure of zebrafish to BaP (10 μM).…”

Section: Zebrafish (Danio Rerio)mentioning

confidence: 99%

Toxicogenomics to Evaluate Endocrine Disrupting Effects of Environmental Chemicals Using the Zebrafish Model

Caballero-Gallardo

Olívero-Verbel

Freeman

2016

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The extent of our knowledge on the number of chemical compounds related to anthropogenic activities that can cause damage to the environment and to organisms is increasing. Endocrine disrupting chemicals (EDCs) are one group of potentially hazardous substances that include natural and synthetic chemicals and have the ability to mimic endogenous hormones, interfering with their biosynthesis, metabolism, and normal functions. Adverse effects associated with EDC exposure have been documented in aquatic biota and there is widespread interest in the characterization and understanding of their modes of action. Fish are considered one of the primary risk organisms for EDCs. Zebrafish (Danio rerio) are increasingly used as an animal model to study the effects of endocrine disruptors, due to their advantages compared to other model organisms. One approach to assess the toxicity of a compound is to identify those patterns of gene expression found in a tissue or organ exposed to particular classes of chemicals, through new technologies in genomics (toxicogenomics), such as microarrays or whole-genome sequencing. Application of these technologies permit the quantitative analysis of thousands of gene expression changes simultaneously in a single experiment and offer the opportunity to use transcript profiling as a tool to predict toxic outcomes of exposure to particular compounds. The application of toxicogenomic tools for identification of chemicals with endocrine disrupting capacity using the zebrafish model system is reviewed.

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“… However, hundreds to thousands of different genes are regulated, either directly or indirectly, by activation of the AhR, which presents major uncertainties in the precise pathway of key events or whether perturbation to multiple pathways is the cause of mortality (Brinkmann et al 2016;Huang et al 2014;Li et al 2013;Whitehead et al 2010).…”

Section: Uncertainitesmentioning

confidence: 99%

“…• High-throughput, next-generation 'OMICs' technologies have identified hundreds to thousands of different genes that are regulated, either directly or indirectly, by the AhR (Brinkmann et al 2016;Huang et al 2014;Li et al 2013;Whitehead et al 2010). These genes include Phase I and Phase II biotransformation enzymes, such as cytochrome P450 1A (CYP1A).…”

Section: Introductionmentioning

confidence: 99%

Adverse Outcome Pathway on aryl hydrocarbon receptor activation leading to early life stage mortality, via increased COX-2

Doering¹,

Hecker

Villeneuve³

et al. 2019

OECD Series on Adverse Outcome Pathways

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Use of toxicogenomics to predict the potential toxic effect of Benzo(a)pyrene on zebrafish embryos: Ocular developmental toxicity

Cited by 54 publications

References 35 publications

Functional Assessment of Cardiac Responses of Adult Zebrafish (Danio rerio) to Acute and Chronic Temperature Change Using High-Resolution Echocardiography

Functional Assessment of Cardiac Responses of Adult Zebrafish (Danio rerio) to Acute and Chronic Temperature Change Using High-Resolution Echocardiography

Toxicogenomics to Evaluate Endocrine Disrupting Effects of Environmental Chemicals Using the Zebrafish Model

Adverse Outcome Pathway on aryl hydrocarbon receptor activation leading to early life stage mortality, via increased COX-2

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