Use of TILLING for Reverse and Forward Genetics of Rice

Rakshit, Sujay; Kanzaki, Hiromitsu; Matsumura, Hideo; Rakshit, Arunita; Fujibe, Takahiro; Okuyama, Yudai; Yoshida, Kentaro; Oli, Muluneh Tamiru; Shenton, Matthew R.; Utsushi, Hiroe; Mitsuoka, Chikako; Abe, Akira; Kiuchi, Y.

doi:10.1002/9783527629398.ch11

Cited by 12 publications

(7 citation statements)

References 14 publications

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“…Here we assume a single thermal component for simplicity. First, consider the antenna temperature measured on-source at a frequency at which only thermal dust continuum emission is observed: Rakshit (1982)…”

Section: A Appendix 1: Derivation Of Radiative Transfer Equationmentioning

confidence: 99%

HERSCHELOBSERVATIONS OF EXTRAORDINARY SOURCES: ANALYSIS OF THE FULLHERSCHEL/HIFI MOLECULAR LINE SURVEY OF SAGITTARIUS B2(N)

Neill¹,

Bergin²,

Lis³

et al. 2014

ApJ

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A sensitive broadband molecular line survey of the Sagittarius B2(N) star-forming region has been obtained with the HIFI instrument on the Herschel Space Observatory, offering the first high-spectral resolution look at this well-studied source in a wavelength region largely inaccessible from the ground (625-157 µm). From the roughly 8,000 spectral features in the survey, a total of 72 isotopologues arising from 44 different molecules have been identified, ranging from light hydrides to complex organics, and arising from a variety of environments from cold and diffuse to hot and dense gas. We present an LTE model to the spectral signatures of each molecule, constraining the source sizes for hot core species with complementary SMA interferometric observations, and assuming that molecules with related functional group composition are cospatial. For each molecule, a single model is given to fit all of the emission and absorption features of that species across the entire 480-1910 GHz spectral range, accounting for multiple temperature and velocity components when needed to describe the spectrum. As with other HIFI surveys toward massive star forming regions, methanol is found to contribute more integrated line intensity to the spectrum than any other species. We discuss the molecular abundances derived for the hot core, where the local thermodynamic equilibrium approximation is generally found to describe the spectrum well, in comparison to abundances derived for the same molecules in the Orion KL region from a similar HIFI survey. Notably, we find significantly higher abundances of amine-and amide-bearing molecules (CH 3 NH 2 , CH 2 NH, and NH 2 CHO) toward Sgr B2(N) than Orion KL, and lower abundances of some complex oxygen-bearing molecules (CH 3 OCHO in particular). In addition to information on the chemical composition of the hot core, the strong far-IR dust continuum allows a number of molecules to be detected in absorption in the Sgr B2(N) envelope for the first time at high spectral resolution, and we discuss the possible physical origin of the kinematic components observed in absorption. Additionally, from the detection of new HOCO + transitions in absorption compared to published HCO + isotopic observations, we -2discuss constraints on the gas-phase CO 2 abundance and compare this to observations of the ice composition in the Galactic Center region, and to CO 2 abundance estimates toward other highmass star-forming regions. The reduced HIFI spectral scan and LTE model are made available to the public as a resource for future investigations of star forming regions in the submillimeter and far-infrared. * Herschel is an ESA space observatory with science instruments provided by European-led Principal Investigator consortia and with important participation from NASA.

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Section: A Appendix 1: Derivation Of Radiative Transfer Equationmentioning

confidence: 99%

HERSCHELOBSERVATIONS OF EXTRAORDINARY SOURCES: ANALYSIS OF THE FULLHERSCHEL/HIFI MOLECULAR LINE SURVEY OF SAGITTARIUS B2(N)

Neill¹,

Bergin²,

Lis³

et al. 2014

ApJ

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“…Immature embryos of cultivar Hitomebore were mutagenized by immersing panicles in 0.015% ethyl methanesulfonate solution (vol/vol) overnight (see ref. 5 for details). The resulting M 1 plants were selfpollinated, and M 2 seeds were obtained.…”

Section: Competing Financial Interestsmentioning

confidence: 99%

Genome sequencing reveals agronomically important loci in rice using MutMap

et al. 2012

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“…In a screen for altered growth phenotypes associated with tiller and leaf angle, we initially selected llb for its increased lamina bending phenotype. Details of the EMS mutagenesis that was carried out at the immature embryo stage are provided elsewhere (Rakshit et al, 2010). Briefly, paddy-field grown plants were transferred to individual pots at the heading stage, after which old and premature flowers were selectively removed, and flowers that opened on the same day were retained for mutagenesis of fertilized egg cells.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

A chloroplast‐localized protein LESION AND LAMINA BENDING affects defence and growth responses in rice

et al. 2016

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SummaryUnderstanding how plants allocate their resources to growth or defence is of long-term importance to the development of new and improved varieties of different crops.Using molecular genetics, plant physiology, hormone analysis and Next-Generation Sequencing (NGS)-based transcript profiling, we have isolated and characterized the rice (Oryza sativa) LESION AND LAMINA BENDING (LLB) gene that encodes a chloroplasttargeted putative leucine carboxyl methyltransferase.Loss of LLB function results in reduced growth and yield, hypersensitive response (HR)-like lesions, accumulation of the antimicrobial compounds momilactones and phytocassanes, and constitutive expression of pathogenesis-related genes. Consistent with these defenceassociated responses, llb shows enhanced resistance to rice blast (Magnaporthe oryzae) and bacterial blight (Xanthomonas oryzae pv. oryzae). The lesion and resistance phenotypes are likely to be caused by the over-accumulation of jasmonates (JAs) in the llb mutant including the JA precursor 12-oxo-phytodienoic acid. Additionally, llb shows an increased lamina inclination and enhanced early seedling growth due to elevated brassinosteroid (BR) synthesis and/or signalling.These findings show that LLB functions in the chloroplast to either directly or indirectly repress both JA-and BR-mediated responses, revealing a possible mechanism for controlling how plants allocate resources for defence and growth.

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Use of TILLING for Reverse and Forward Genetics of Rice

Cited by 12 publications

References 14 publications

HERSCHELOBSERVATIONS OF EXTRAORDINARY SOURCES: ANALYSIS OF THE FULLHERSCHEL/HIFI MOLECULAR LINE SURVEY OF SAGITTARIUS B2(N)

HERSCHELOBSERVATIONS OF EXTRAORDINARY SOURCES: ANALYSIS OF THE FULLHERSCHEL/HIFI MOLECULAR LINE SURVEY OF SAGITTARIUS B2(N)

Genome sequencing reveals agronomically important loci in rice using MutMap

A chloroplast‐localized protein LESION AND LAMINA BENDING affects defence and growth responses in rice

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