Use of thyroid transcription factor 1, PE-10, and cytokeratins 7 and 20 in discriminating between primary lung carcinomas and metastatic lesions in fine-needle aspiration biopsy specimens

Chhieng, David C.; Cangiarella, Joan; Zakowski, Maureen F.; Goswami, Sunanda; Cohen, Jean‐Marc; Yee, Herman

doi:10.1002/cncr.9048

Cited by 144 publications

(96 citation statements)

References 43 publications

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“…The immunohistochemical detection for the nuclear expression of TTF-1 is considered as a useful marker in favor of lung or thyroid origin because of its high specificity (95-100%). [9][10][11][12] In the literature, TTF-1 expression in nuclei has been shown in rare cases of adenocarcinomas arising outside from lung or thyroid: one weakly positive case of metastatic ovarian carcinoma 13 and one focal positive case of endometrial carcinoma. 14 Recently, our group has shown one positive adenocarcinoma of colorectal origins, 8 whereas no such positivity was reported in literature ( Table 2).…”

Section: Discussionmentioning

confidence: 99%

Variable sensitivity and specificity of TTF-1 antibodies in lung metastatic adenocarcinoma of colorectal origin

et al. 2005

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Thyroid transcription factor-1 (TTF-1) is considered as a reliable marker for differential diagnosis in distinguishing primary adenocarcinomas of the lung from extrathoracic origins. We previously reported the first case of lung metastasis of colorectal origin, with nuclear expression of TTF-1. As most previous studies were performed on series of extrathoracic primary tumors, we raised the question of a possible role of lung microenviroment in TTF-1 expression. We investigated the rate of TTF-1 expression in lung metastases of extrathoracic adenocarcinomas and compared results of immunohistochemistry performed with different primary antibodies. Two different clones of antibodies (8G7G1/1 from Dako, SPT24 from Novocastra) raised against TTF-1 were used on 56 lung-metastatic malignant tumors, 41 from colorectal origin. A series of primary colorectal (90 cases) and primary pulmonary adenocarcinomas (86 cases) were also investigated. Four of 41 (10%) lung metastases of colorectal adenocarcinomas displayed a nuclear staining for TTF-1 with SPT24 clone. Three of the four positive cases displayed similar nuclear staining in primary and/or other extrathoracic metastatic sites as well as four of 90 (5%) primary colorectal adenocarcinomas, ruling out the role of lung microenvironment. None of them was positive with 8G7G1/1 clone. Sensitivity between two sets of antibodies was compared in 86 primary pulmonary adenocarcinomas. Nuclear staining was detected in 72 cases (84%) with Novocastra's antibody and 56 cases (65%) with Dako's. Significant discordance was observed (Po 0.01). These results suggest that the diagnostic virtue of TTF-1 detection depends on the used antibody's clone. The SPT24 clone seems to have a stronger affinity for TTF-1 protein but may lead to a few positive colorectal adenocarcinomas. Keywords: TTF-1; lung; carcinoma; metastasis; colorectal Thyroid transcription factor-1 (TTF-1) is a tissuespecific transcription factor expressed in the epithelial cells of thyroid and lung (type II pneumocytes and Clara cells). Carcinomas arising from lung and thyroid also show frequent TTF-1 expression. 1,2 As the lung is one of the most common sites of metastasis, TTF-1 is considered as a reliable marker to distinguish between primary lung carcinoma and lung metastasis, especially when dealing with an adenocarcinoma or a large-cell carcinoma. [3][4][5][6] It is also considered as a reliable marker in the differential diagnosis between pleural localization of lung origin carcinoma and malignant mesothelioma. 7 Two main commercial available clones of monoclonal antibodies have been raised against TTF-1 for immunohistochemical use, 8G7G1/1 and SPT24, their diagnostic value in determining the lung or thyroid origin of adenocarcinoma has not yet been compared. Most of the studies show the high specificity of TTF-1 detection and have been published used the 8G7G1/1 clone. We previously reported one case of lung metastasis of colorectal origin, which showed a focal nuclear staining with the anti-TTF-1 antibody (clone SPT24)...

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Section: Discussionmentioning

confidence: 99%

Variable sensitivity and specificity of TTF-1 antibodies in lung metastatic adenocarcinoma of colorectal origin

et al. 2005

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“…However, most of them are not specific for lung AdC but for epithelial cells or cancers of epithelial cell origin in general. Even for tissue-specific differentiation markers, such as the surfactant pulmonary-associated proteins A, B and C (SFTPA2, SFTPB and SFTPC, respectively) and thyroid transcription factor-1 (TTF-1), sensitivity as well as specificity is not high enough to use for molecular diagnosis (Chhieng et al, 2001;Zamecnik and Kodet, 2002). Thus, it is absolutely necessary to search for more specific and highly sensitive markers that will be useful for molecular diagnosis of lung AdC.…”

Section: Introductionmentioning

confidence: 99%

Identification of tumor markers and differentiation markers for molecular diagnosis of lung adenocarcinoma

Nakamura¹,

Kobayashi²,

Nakamoto³

et al. 2006

Oncogene

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“…Negative staining for TTF-1 and surfactant apoprotein suggests that the adenocarcinoma did not arise from the lung, while negative staining for cytokeratin 7 and positive staining for cytokeratin 20 and CD10 suggest an adenocarcinoma of intestinal origin. 9,10) Immunostaining is very useful in differentiating between primary and metastatic lung cancer.…”

Section: Discussionmentioning

confidence: 99%

Solitary Pulmonary Metastasis from Carcinoma of the Papilla of Vater

Goto

Urakami

Akanabe

et al. 2011

ATCS

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Pulmonary metastasis from carcinoma of the papilla of Vater is considered to be a late event, and patients can be treated with radiotherapy, chemotherapy, or palliative surgery. However, surgical treatment of an isolated lung metastasis has not been reported. We present a surgical case of solitary pulmonary metastasis from carcinoma of the papilla of Vater. A 51-yearold man underwent pylorus-preserving pancreaticoduodenectomy for Vater carcinoma. During follow-up, chest computed tomography revealed a nodular shadow in the right lung. The pathological examination demonstrated the appearance of the pulmonary tumor resembled that of the previously resected Vater carcinoma, and both tumors showed similar immunostaining properties, leading to the pathological diagnosis of pulmonary metastasis from carcinoma of the papilla of Vater. Isolated pulmonary metastasis from carcinoma of the papilla of Vater is extremely rare, but surgery could be the treatment of choice.

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Use of thyroid transcription factor 1, PE-10, and cytokeratins 7 and 20 in discriminating between primary lung carcinomas and metastatic lesions in fine-needle aspiration biopsy specimens

Cited by 144 publications

References 43 publications

Variable sensitivity and specificity of TTF-1 antibodies in lung metastatic adenocarcinoma of colorectal origin

Variable sensitivity and specificity of TTF-1 antibodies in lung metastatic adenocarcinoma of colorectal origin

Identification of tumor markers and differentiation markers for molecular diagnosis of lung adenocarcinoma

Solitary Pulmonary Metastasis from Carcinoma of the Papilla of Vater

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