Use of thiolated oligonucleotides as anti-fouling diluents in electrochemical peptide-based sensors

McQuistan, Adam; Zaitouna, Anita; Echeverría, Elena; Lai, Rebecca Y.

doi:10.1039/c4cc01290a

Cited by 43 publications

(52 citation statements)

References 24 publications

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“…Several surface modification strategies have been used to fabricate E-PB sensors to date. McQuistan et al [142] reported E-PB HIV sensor fabrication using thiolated oligonucleotides as passivating diluents and its applicability was examined in synthetic human saliva. Although the researchers fabricated several sensitive and selective electrochemical biosensors to target salivary Abs detection, there is still a need for improvement.…”

Section: Salivary Antibodiesmentioning

confidence: 99%

Saliva-Based Biosensors: Noninvasive Monitoring Tool for Clinical Diagnostics

Malon

Sadir

Malarvili

et al. 2014

BioMed Research International

176

108

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Saliva is increasingly recognised as an attractive diagnostic fluid. The presence of various disease signalling salivary biomarkers that accurately reflect normal and disease states in humans and the sampling benefits compared to blood sampling are some of the reasons for this recognition. This explains the burgeoning research field in assay developments and technological advancements for the detection of various salivary biomarkers to improve clinical diagnosis, management, and treatment. This paper reviews the significance of salivary biomarkers for clinical diagnosis and therapeutic applications, with focus on the technologies and biosensing platforms that have been reported for screening these biomarkers.

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Section: Salivary Antibodiesmentioning

confidence: 99%

Saliva-Based Biosensors: Noninvasive Monitoring Tool for Clinical Diagnostics

Malon

Sadir

Malarvili

et al. 2014

BioMed Research International

176

108

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“…While the probe coverages shown in Table S2 are substantially lower than the coverages used in the E-DNA and electrochemical aptamer-based (E-AB) sensors, they are ideal for capturing large targets such as the Anti-p24 Ab. [4][5][6][39][40][41] We also evaluated the ET rate of MB before and after the addition of 70 nM Anti-p24 Ab and fitted the data to a previously reported model ( Figure S6). 29,42,43 We were not able to obtain a good fit for the C6-OH sensor, presumably because of the different conformations this peptide 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 14 probe can assume, resulting in a distribution of local redox environments and ET rates.…”

Section: Sensor Designmentioning

confidence: 99%

“…The similarity in K d values further demonstrates that the incorporation of these antifouling diluents does not negatively affect probe-target interactions.Sensor SelectivitySensor selectivity is one of the most important aspects in the development of reagentless and user-friendly sensors. While the E-DNA and E-AB sensors have shown to work well in a wide range of complex media, 37,41 the E-PB sensors have only been demonstrated to function well in 10 % saliva and 50 % urine proxy 4,6. It is necessary to improve sensor selectivity to broaden the applicability of E-PB sensors and make possible use in clinical settings.…”

mentioning

confidence: 95%

Comparison of Mannose, Ethylene Glycol, and Methoxy-Terminated Diluents on Specificity and Selectivity of Electrochemical Peptide-Based Sensors

et al. 2015

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We report the synthesis and application of three new antifouling diluents for the fabrication of an E-PB HIV sensor. Among the three thiolated antifouling diluents used in this study, the methoxy-terminated diluent (C6-MEG) is the most effective in alleviating both nonspecific binding and adsorption of matrix contaminants onto the sensor surface, especially when compared to the mannose- (C6-MAN) and ethylene-glycol-terminated (C6-EG) diluents. The sensor fabricated with C6-MEG has a specificity factor (∼13.5) substantially higher than the sensor passivated with only 6-mercapto-1-hexanol (∼1.5). It is functional even when employed directly in 25% serum, an achievement that has not been observed with this class of E-PB sensors. More importantly, incorporation of these antifouling diluents has negligible impact on other important sensor properties such as sensitivity and binding kinetics. This sensor passivation strategy is versatile and can potentially be used with other E-PB sensors, as well as surface-based sensors that utilize thiol-gold self-assembled monolayer chemistry.

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“…An electrochemical peptide-based sensor for HIV antibodies detection was developed by McQuistan et al [50] by self-assembling a specific thiolated peptide labeled with MB as electrochemical indicator, and short thiolated DNAs as anti-fouling diluents on gold electrodes (Figure 3). The recognition between the peptide probe and the antibody damped the probe flexibility and this limited surface mobility was followed by the decrease in the MB reduction peak obtained by alternating current voltammetry (ACV).…”

Section: Electrochemical Genosensing Of Circulating Biomarkersmentioning

confidence: 99%

Electrochemical Genosensing of Circulating Biomarkers

Campuzano

Yáñez‐Sedeño

Pingarrón

2017

Sensors

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Management and prognosis of diseases requires the measurement in non- or minimally invasively collected samples of specific circulating biomarkers, consisting of any measurable or observable factors in patients that indicate normal or disease-related biological processes or responses to therapy. Therefore, on-site, fast and accurate determination of these low abundance circulating biomarkers in scarcely treated body fluids is of great interest for health monitoring and biological applications. In this field, electrochemical DNA sensors (or genosensors) have demonstrated to be interesting alternatives to more complex conventional strategies. Currently, electrochemical genosensors are considered very promising analytical tools for this purpose due to their fast response, low cost, high sensitivity, compatibility with microfabrication technology and simple operation mode which makes them compatible with point-of-care (POC) testing. In this review, the relevance and current challenges of the determination of circulating biomarkers related to relevant diseases (cancer, bacterial and viral infections and neurodegenerative diseases) are briefly discussed. An overview of the electrochemical nucleic acid–based strategies developed in the last five years for this purpose is given to show to both familiar and non-expert readers the great potential of these methodologies for circulating biomarker determination. After highlighting the main features of the reported electrochemical genosensing strategies through the critical discussion of selected examples, a conclusions section points out the still existing challenges and future directions in this field.

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Use of thiolated oligonucleotides as anti-fouling diluents in electrochemical peptide-based sensors

Cited by 43 publications

References 24 publications

Saliva-Based Biosensors: Noninvasive Monitoring Tool for Clinical Diagnostics

Saliva-Based Biosensors: Noninvasive Monitoring Tool for Clinical Diagnostics

Comparison of Mannose, Ethylene Glycol, and Methoxy-Terminated Diluents on Specificity and Selectivity of Electrochemical Peptide-Based Sensors

Electrochemical Genosensing of Circulating Biomarkers

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