Use of the USDT flow immunosensor for quantitation of benzoylecgonine in urine

Yu, Hao; Kusterbeck, Anne W.; Hale, Mary J.; Ligler, Frances S.; Whelan, James P.

doi:10.1016/0956-5663(96)85923-9

Cited by 25 publications

(15 citation statements)

References 16 publications

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“…Over time the signal does decrease due to displacement of labeled antigen by antigen in the samples (27) and due to leaching of labeled antigen from the immobilized antibody (28). The baseline shows a slow but steady decrease that is characteristic of displacement assay data.…”

Section: Discussionmentioning

confidence: 94%

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Multianalyte Detection Using a Capillary-Based Flow Immunosensor

Narang

Gauger²,

Kusterbeck

et al. 1998

Analytical Biochemistry

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Section: Discussionmentioning

confidence: 94%

“…The slow leaching of antibody-bound labeled antigen is characteristic of the displacement system and may extend over a 4-day period before depletion of the labeled antigen reduces the signal beyond acceptable levels (28). We can also perform simple point calibrations to adjust for the loss of labeled antigen.…”

Section: Discussionmentioning

confidence: 98%

Multianalyte Detection Using a Capillary-Based Flow Immunosensor

Narang

Gauger²,

Kusterbeck

et al. 1998

Analytical Biochemistry

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“…Several excellent reviews have been published to give a broad insight into conventional and emerging techniques for the detection of illegal drugs and their metabolites. Reviews or other papers covering the detection of drugs, e.g., via the testing of hair [ 6 , 7 , 8 , 9 ], the use of molecularly imprinted polymers [ 10 , 11 ], aptamers [ 12 ], ion mobility spectrometry [ 10 , 11 ], mass spectrometry [ 13 , 14 , 15 , 16 , 17 ], hybrid methods [ 18 ], electrochemical methods [ 19 , 20 ], biosensors [ 12 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ], and immunoassays [ 35 , 36 , 37 ] have been published. Quite popular in analytical chemistry was the detection of cocaine on banknotes [ 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 ].…”

Section: Introductionmentioning

confidence: 99%

Cocaine Detection by a Laser-Induced Immunofluorometric Biosensor

et al. 2021

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The trafficking of illegal drugs by criminal networks at borders, harbors, or airports is an increasing issue for public health as these routes ensure the main supply of illegal drugs. The prevention of drug smuggling, including the installation of scanners and other analytical devices to detect small traces of drugs within a reasonable time frame, remains a challenge. The presented immunosensor is based on a monolithic affinity column with a large excess of immobilized hapten, which traps fluorescently labeled antibodies as long as the analyte cocaine is absent. In the presence of the drug, some binding sites of the antibody will be blocked, which leads to an immediate breakthrough of the labeled protein, detectable by highly sensitive laser-induced fluorescence with the help of a Peltier-cooled complementary metal-oxide-semiconductor (CMOS) camera. Liquid handling is performed with high-precision syringe pumps and microfluidic chip-based mixing devices and flow cells. The biosensor achieved limits of detection of 7 ppt (23 pM) of cocaine with a response time of 90 s and a total assay time below 3 min. With surface wipe sampling, the biosensor was able to detect 300 pg of cocaine. This immunosensor belongs to the most sensitive and fastest detectors for cocaine and offers near-continuous analyte measurement.

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“…Several excellent reviews have been published to give a broad insight over conventional and emerging techniques for the detection of illegal drugs and their metabolites. Reviews or other papers covering the detection of drugs, e.g via the testing of hair [3][4][5][6], the use of molecularly imprinted polymers [7,8], aptamers [9], ion mobility spectrometry [10,11], mass spectrometry [12][13][14][15][16], hybrid methods [17], electrochemical methods [18,19], biosensors [9,[20][21][22][23][24][25][26][27][28][29][30][31][32][33] and immunoassays [34][35][36] have been published. Quite popular in analytical chemistry was the detection of cocaine on banknotes [37][38][39][40][41][42][43][44].…”

Section: Introductionmentioning

confidence: 99%

Cocaine Detection by a Laser-induced Immunofluorometric Biosensor

Paul¹,

Tannenberg²,

Tscheuschner³

et al. 2021

Preprint

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The trafficking of illegal drugs by criminal networks at borders, harbors, or airports is an increasing issue in public health as these routes ensure the main supply of illegal drugs. The prevention of drug smuggling, including the installation of scanners and other analytical devices to detect ultra-small traces of drugs within a reasonable time frame, remains a challenge. The presented immunosensor is based on a monolithic affinity column with a large excess of immobilized hapten, which traps fluorescently labeled antibodies as long as the analyte cocaine is absent. In the presence of the drug, some binding sites of the antibody will be blocked, which leads to an immediate breakthrough of the labeled protein, detectable by highly sensitive laser-induced fluorescence with the help of a Peltier-cooled complementary metal-oxide-semiconductor (CMOS) camera. Liquid handling is performed with high-precision syringe pumps and microfluidic chip-based mixing devices and flow cells. The biosensor achieved limits of detection of 23 pM (7 ppt) of cocaine with a response time of 90 seconds and a total assay time below 3 minutes. With surface wipe sampling, the biosensor was able to detect 300 pg of cocaine. This immunosensor belongs to the most sensitive and fastest detectors for cocaine and offers near-continuous analyte measurement.

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Use of the USDT flow immunosensor for quantitation of benzoylecgonine in urine

Cited by 25 publications

References 16 publications

Multianalyte Detection Using a Capillary-Based Flow Immunosensor

Multianalyte Detection Using a Capillary-Based Flow Immunosensor

Cocaine Detection by a Laser-Induced Immunofluorometric Biosensor

Cocaine Detection by a Laser-induced Immunofluorometric Biosensor

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