Use of the Scanning Electron Microscope in Morphological Studies of Copepods 1)

Grice, George D.; Lawson, Thomas J.

doi:10.1163/156854071x00292

Cited by 8 publications

(5 citation statements)

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“…With the exception of the latter two studies, qualitative descriptions and characterization of the different internal foregut structures have been primarily based on light microscopy. These studies may misinterpret the internal arrangement, topography, and three-dimensional orientation of the internal armature of the foregut, mainly due to problems with depth of field (Grice & Lawson, 1971). Oshel & Steele (1988), from SEM observations, briefly described some foregut features of Gnathophausia ingens.…”

Section: Introductionmentioning

confidence: 99%

Comparative foregut functional morphology of three co-occurring mysids (Crustacea: Mysidacea) from south-eastern Tasmania

Metillo

Ritz

1994

J. Mar. Biol. Ass.

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Studies of mysid diets by gut contents analysis have generally revealed a broadly omnivorous feeding habit, but there are also tendencies towards carnivory, herbivory and/or detritivory (e.g. Nath & Pillai, 1973; Siegfried & Kopache, 1980; Mauchline, 1980; Zagursky & Feller, 1985; Wooldridge & Bailey, 1982; Webb & Wooldridge, 1989). Examination of feeding structures is also necessary to support inferences about feeding ecology (e.g. Webb & Wooldridge, 1989). However, there have been few studies relating to the functional morphology of mysid foreguts (Gelderd, 1909; Haffer, 1965; Nath & Pillai, 1973; Mauchline, 1980; Friesen et al., 1986; Webb & Wooldridge, 1989; Storch, 1989). With the exception of the latter two studies, qualitative descriptions and characterization of the different internal foregut structures have been primarily based on light microscopy. These studies may misinterpret the internal arrangement, topography, and three-dimensional orientation of the internal armature of the foregut, mainly due to problems with depth of field (Grice & Lawson, 1971). Oshel & Steele (1988), from SEM observations, briefly described some foregut features of Gnathophausia ingens. In a comparative study, Storch (1989), using the techniques of transmission and scanning electron microscopy, described in detail the different food chambers and channels, cuticular ridges, and ultrastructure of the epithelial and cuticular linings of the mysid foregut. Webb & Wooldridge (1989) noted the strong relationship between mouthparts, foregut morphology, and the feeding habits of two co-occurring mysids.

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Section: Introductionmentioning

confidence: 99%

Comparative foregut functional morphology of three co-occurring mysids (Crustacea: Mysidacea) from south-eastern Tasmania

Metillo

Ritz

1994

J. Mar. Biol. Ass.

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“…Many anatomical descriptions of lernaeopodids including Clavella were traditionally based on individually fixed dissections, cleared in lactic acid and illustrated from camera lucida-assisted drawings with phase-contrast illumination and interference techniques for finer features [ 8 , 18 , 22 , 25 ]. In the present study, the application of high-resolution SEM allowed for new visualization of distinct anatomical features of the secondary antenna, the bulla complex, and components of the oral cone.…”

Section: Discussionmentioning

confidence: 99%

“…SEM is well accepted as an effective tool for study of the morphological characteristics of copepods and small related crustaceans, including the parasites [ 2 , 14 , 25 – 30 ]. Traditional SEM preparation protocols are known to be particularly rigorous and time-consuming and thus not always considered routine for the quick preparation of small delicate organisms due to their structural complexity and relatively delicate anatomy [ 14 , 26 ].…”

Section: Discussionmentioning

confidence: 99%

A Redescription of the Bulla, Antennae, and Mouth Parts of Female Clavella sp. (Copepoda: Siphonostomatoida: Lernaeopodidae) Infesting Wild Gadus morhua Using Scanning Electron Microscopy (SEM)

Murray

Hanlon

Marshall

et al. 2020

Journal of Parasitology Research

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Many members of the Copepod family Lernaeopodidae are well-known parasites of gadids. This study reports on the occurrence of a lernaeopodid infestation of wild-sourced Gadus morhua sampled from separate inshore (Gilbert Bay, NL) and offshore (Virgin Rocks, NL) populations from Newfoundland, Canada. The majority of the parasites were observed to be associated with the buccal cavity, gill filaments, gill arch, and occasionally near the outer edge of the operculum. Anatomical analysis and detailed redescriptions of the parasite’s functional anatomy (mouth parts, antennae, and bulla complex) using high-resolution SEM indicated that the parasite was most likely of the genus Clavella. New morphological details of the second antennae ornamentation, first maxillae, bulla complex, and the oral cone are provided and discussed with regard to their potential in taxonomic applications.

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“…Scanning Electron microscopy (SEM) is well accepted as one of the most important methods for study of the morphologic characteristics of copepods and related small crustaceans, including the parasites (Grice and Lawson, ; Pulsifer, ). Unfortunately, morphological studies of the Ergasilidae using SEM techniques are rare.…”

Section: Introductionmentioning

confidence: 99%

The external morphology of adult female Egrasilus labracis as shown using hexamethyldisilazane treated, uncoated specimens for scanning electron microscopy

Murray

Hill

Ang

2016

Microscopy Res & Technique

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The description and application of a modified Scanning Electron Microscope preparation technique using hexamethyldisilazane for small parasitic copepods was demonstrated though a high resolution depiction of individuals of Ergasilus labracis sampled from three spined stickleback (Gasterosteus aculeatus) in Bay D'Espoir, Newfoundland during summer 2015 and from archival samples retrieved from Atlantic salmon par (Salmo salar) stored at the Atlantic reference centre, St. Andrews, New Brunswick. The specimens were very well preserved showing high quality detail of important features and verifying those previously described using light microscopy by Hogans. Additionally the technique allowed excellent in situ demonstrations of mouth parts, swimming legs, and unusual and previously undescribed features of the second antenna including prominent striations and pore-like structures found to define the claw. It is thought that this technique will become a quick and efficient tool for describing important taxonomic features of small parasitic copepods like E. labracis or other similar small aquatic organisms. Microsc. Res. Tech. 79:657-663, 2016. © 2016 Wiley Periodicals, Inc.

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Use of the Scanning Electron Microscope in Morphological Studies of Copepods 1)

Cited by 8 publications

References 0 publications

Comparative foregut functional morphology of three co-occurring mysids (Crustacea: Mysidacea) from south-eastern Tasmania

Comparative foregut functional morphology of three co-occurring mysids (Crustacea: Mysidacea) from south-eastern Tasmania

A Redescription of the Bulla, Antennae, and Mouth Parts of Female Clavella sp. (Copepoda: Siphonostomatoida: Lernaeopodidae) Infesting Wild Gadus morhua Using Scanning Electron Microscopy (SEM)

The external morphology of adult female Egrasilus labracis as shown using hexamethyldisilazane treated, uncoated specimens for scanning electron microscopy

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