2019
DOI: 10.1590/abd1806-4841.20197929
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Use of the polymerase chain reaction for the diagnosis of American tegumentary leishmaniasis

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“…The second sample was used for imprint with staining, which also revealed no amastigote forms of the parasite. Subsequently, the same material was preserved in sterile saline solution, macerated with 300μg of extraction buffer, and frozen in a properly identified flask for PCR as described by Barçante et al, 2019. After analyzing the electrophoretic pattern of the sample with the positive control, the presence of Leishmania spp.…”
Section: Introductionmentioning
confidence: 99%
“…The second sample was used for imprint with staining, which also revealed no amastigote forms of the parasite. Subsequently, the same material was preserved in sterile saline solution, macerated with 300μg of extraction buffer, and frozen in a properly identified flask for PCR as described by Barçante et al, 2019. After analyzing the electrophoretic pattern of the sample with the positive control, the presence of Leishmania spp.…”
Section: Introductionmentioning
confidence: 99%