Use of the Ogawa-Kudoh method to isolate mycobacteria in a tuberculosis reference laboratory in northwestern Paraná, Brazil

Silva, Fernanda Schaefer Borges da; Castilho, Aline Lemes; Maltempe, Flaviane Granero; Pina, Rosângela Zampieri; Takao, Elisa Keiko Hirayama; Siqueira, Vera Lúcia Dias; Cardoso, Rosilene Fressatti; Caleffi-Ferracioli, Katiany Rizzieri

doi:10.1590/s1984-82502013000300018

Cited by 3 publications

(1 citation statement)

References 5 publications

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“…Silva et al, ( 13 ) evaluated a 8-year period of use of the Ogawa-Kudoh method in the routine of a reference laboratory in the Northeastern region of the State of Paraná, and obtained results that allowed concluding the technique is an excellent tool for early diagnosis of pulmonary TB. Some studies from other countries, such as those conducted in Venezuela, Uruguay, and Gambia, also demonstrated satisfactory results and adjustment of the Ogawa-Kudoh method to the needs and infrastructure of these different locations.…”

Section: Discussionmentioning

confidence: 99%

Comparison between Ogawa-Kudoh and modified Petroff techniques for mycobacteria cultivation in the diagnosis of pulmonary tuberculosis

Costa

Silva

Fochat

et al. 2018

Einstein (São Paulo)

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Objective To compare the performance of the Ogawa-Kudoh method with the modified Petroff technique in diagnosis of pulmonary tuberculosis.Methods A total of 205 sputum samples from 166 patients with clinical suspicion or under pulmonary tuberculosis follow-up, seen at a public tertiary care hospital, from July 2014 to July 2016 were used. All samples were simultaneously processed using the Ogawa-Kudoh and modified Petroff decontamination methods, according to the recommendations of the Ministry of Health. In the statistical analysis, the McNemar test and the Kappa index were used, respectively, to compare proportions and verify agreement between data.Results The Ogawa-Kudoh and modified Petroff methods were efficient in mycobacteria detection, with no significant differences in results (p=0.549) and contamination rate of the cultures (p=0.065). The agreement between techniques was considered excellent (Kappa index of 0.877) and Ogawa-Kudoh, as compared to the modified Petroff technique, showed sensitivity of 90.4%, specificity of 96.6%, positive predictive value of 94.3% and negative predictive value of 94.2%.Conclusion The Ogawa-Kudoh technique proved to be sufficiently sensitive and specific for diagnosis of pulmonary tuberculosis, and, therefore, suitable for routine laboratory application. Since it is simple, low-cost and has less technical requirements for biosafety and professional training, Ogawa-Kudoh is an alternative for managers and healthcare professionals to promote the expansion of bacteriological diagnostic coverage of pulmonary tuberculosis.

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Section: Discussionmentioning

confidence: 99%

Comparison between Ogawa-Kudoh and modified Petroff techniques for mycobacteria cultivation in the diagnosis of pulmonary tuberculosis

Costa

Silva

Fochat

et al. 2018

Einstein (São Paulo)

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Assessing the Performance of GOLD, Glide and MM-GBSA on a Dataset of Hydrazide-hydrazone-based Tuberculostatics

Mateev

Georgieva

Zlatkov

2023

LDDD

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Background: Tuberculosis is considered as a global health problem, hence the screening and synthesis of novel tuberculostatic drugs is a necessity. Molecular docking could drastically reduce the time of hit identification, however initial validation is required to reduce the false-positive results. Objective: Assessment of several searching and scoring algorithms towards a custom dataset of hydrazide-hydrazone-based tuberculostatics was conducted to obtain a reliable docking protocol for future virtual screening. Methods: Modification in the scoring functions, size of the grid space, and presence of active waters of a GOLD 5.3 docking protocol was conducted. Subsequently, side chain flexibility and ensemble docking were carried out to assess the role of protein flexibility in the correlation coefficient. In addition, docking simulations with Glide and free binding energy calculations with MM-GBSA were implemented. The Pearson correlation coefficient between the experimental and the acquired in silico data was calculated after each step of this work. The major interactions between the top scored ligands and the active site of 2X22 were visualized applying Discovery Studio. Results: An optimized GOLD 5.3 docking protocol led to a drastically enhanced Pearson correlation coefficient of the training set – from 0.461 to 0.823, as well as an excellent pairwise correlation coefficient in the test set - 0,8405. Interestingly, the Glide docking scores and the free binding energy calculations with MM-GBSA did not achieve reliable results. During the visualization of the top ranked compounds, it was observed that Lys165 played a major role in the formation of stable complexes. Conclusion: It could be concluded that the performance of the optimized GOLD 5.3 docking protocol demonstrated significantly higher reliability against the hydrazide-hydrazone dataset, when compared to Glide docking simulations and MM-GBSA free binding energy calculations. The results could be utilized for future virtual screenings.

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Perfil epidemiológico da tuberculose em Juiz de Fora, município prioritário no estado de Minas Gerais

Rêgo

Assis

Costa

2019

HU Rev

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Introdução: A tuberculose (TB) é considerada um dos maiores problemas mundiais de saúde. Visando aeliminação dessa doença, a Organização Mundial de Saúde (OMS) elaborou um plano, chamado “The endTB strategy”, cujas metas propostas foram adotadas pelo Ministério da Saúde. Juiz de Fora é a segundacidade do estado de Minas Gerais com os maiores números de casos dessa doença sendo consideradamunicípio prioritário para controle da TB. Objetivo: Realizar uma análise do perfil epidemiológico da TBna cidade de Juiz de Fora nos anos de 2008 a 2017, comparando os dados registrados a nível nacional,estadual e municipal, bem como avaliar o cenário municipal frente às metas propostas pelo plano deenfrentamento da TB. Material e métodos: Foi realizado um estudo transversal, descritivo, de sérietemporal utilizando dados secundários disponibilizados pelo Departamento de Informática do SistemaÚnico de Saúde (DATASUS). Resultados: O coeficiente de incidência e mortalidade da TB assim comoa taxa de abandono de tratamento verifi cados em Juiz de Fora, de 2008-2017, superaram as médiasnacionais e estaduais, além de extrapolarem as metas previstas no plano de enfrentamento da TB.Cabe ressaltar, no entanto, que a proporção de teste rápido de HIV e o número de cultura de escarrorealizados entre os casos de retratamento de TB superaram as médias nacional e estadual. Conclusão:O perfi l epidemiológico da TB em Juiz de Fora revelou uma situação em alguns aspectos piores emrelação ao cenário nacional e estadual, mas também evidenciou que medidas já estão sendo realizadaspelo município para o enfrentamento da doença.

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Use of the Ogawa-Kudoh method to isolate mycobacteria in a tuberculosis reference laboratory in northwestern Paraná, Brazil

Cited by 3 publications

References 5 publications

Comparison between Ogawa-Kudoh and modified Petroff techniques for mycobacteria cultivation in the diagnosis of pulmonary tuberculosis

Comparison between Ogawa-Kudoh and modified Petroff techniques for mycobacteria cultivation in the diagnosis of pulmonary tuberculosis

Assessing the Performance of GOLD, Glide and MM-GBSA on a Dataset of Hydrazide-hydrazone-based Tuberculostatics

Perfil epidemiológico da tuberculose em Juiz de Fora, município prioritário no estado de Minas Gerais

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