Use of the liquid scintillation spectrometer for determining adenosine triphosphate by the luciferase enzyme

Stanley, Philip E.; Williams, S. G.

doi:10.1016/0003-2697(69)90323-6

Cited by 879 publications

(247 citation statements)

References 10 publications

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“…Glucose was estimated by two methods: (1) o-toluidine method (Cooper and McDaniel, 1970); (2) Boehringer GOD/period method for blood glucose. Lactate was estimated by a Boehringer enzymic kit and adenosine triphosphate (ATP) was measured by the method of Stanley and Williams (1969). Changes in the concentration of glucose, lactate and ATP during incubation are expressed as Mg/h/lO^ erythrocytes.…”

Section: Biochemical Estimationsmentioning

confidence: 99%

METABOLISM OF BABESIA PARASITES IN VITRO. GLUCOSE AND ENERGY METABOLISM AND SURVIVAL OF BABESIA RODHAINI IN A BASAL MEDIUM WITH AND WITHOUT ADENOSINE

1982

Aust J Exp Biol Med

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Summary.During in vitro incubation in Krebs Ringer phosphate (KRP) medium, rat erythrocytes about 20% parasitized with B. rodhaini used two to four times as much gtucose as unparasitized erythrocytes. However, the ATP concentration and ATP production of parasitized erythrocytes decreased. For example, before incubation the ATP concentration in parasitized erythrocytes was significantly higher than unparasitized erythrocytes, but following 0-5 h incubation it was reduced to about the same as in unparasitized erythrocytes.Addition of 5 X 10-3 M adenosine to the basal medium increased the ATP production and the adenylate energy charge of parasitized erythrocytes. Infectivity tests in susceptible mice showed that, despite these seemingly favourable changes in energy metabolism, adenosine had no detectable effect on the survival of B. rodhaini. Absence of infectivity showed the inability of KRP medium to support B. rodhaini for 22 h in vitro with or without adenosine.Adenosine also greatly decreased the glucose uptake by parasitized erythrocytes, apparently by competitive inhibition, since lactate production was unchanged. INTRODUCTIONStudies of the metabolism of haemoprotozoan parasites of the genus Babesia are relevant to the production of living vaccines against bovine infections (Callow, 1977). The present series of investigations was designed to study the in vitro metabolism and survival in basal salts media of Babesia rodhaini.Metabolic studies of Babesia rodhaini (Rickard, 1969;Momen, 1979) provided information on the in vitro metabolism, particularly carbohydrate metabolism, of the parasite in basal salts media. There was no information, however, on the in vitro survival or energy metabolism of the parasite in basal media.

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Section: Biochemical Estimationsmentioning

confidence: 99%

METABOLISM OF BABESIA PARASITES IN VITRO. GLUCOSE AND ENERGY METABOLISM AND SURVIVAL OF BABESIA RODHAINI IN A BASAL MEDIUM WITH AND WITHOUT ADENOSINE

1982

Aust J Exp Biol Med

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“…ATP assays were made by the niethod of Stanley and Williams (1969). The luciferase enzyme solution was prepared freshly each day by homogenising desiccated firefly lanterns in ice-cold 0-1 M sodium arsenate buffer, pH 7-4, containing 40 niM MgSO4 using a 2 ml Dounce-type homogeniser.…”

Section: Atp Assaymentioning

confidence: 99%

Copper Deficiency in the Rat. Effect on Adenine Nucleotide Metabolism

Gallagher

Reeve

1971

Aust J Exp Biol Med

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Summary. Copper deficiency does not influence the synthesis of ATP by liver mitochondria noc does it alter the concentration of ATP in whole liver homogenates or in isolated mitochondria, microsomes jmd supernatant. However, copper deficiency considerably reduces the affinity of mitochondria to biad adenine nucleotides on membrane preparations. This defect leads to failure of ATP transport across the inner membrane resulting in abnormal accumulation of ATP within rliitochondria incubated in vitro and a requirement of exogenous ATP for optimal rates of phospholipid synthesis by enzymes of the outer mitochondrial membrane. The effect of copper deficiency on adenine nucleotide metabolism and consequently on phospholipid synthesis is analogous to that of the glycoside atractyloside. The possible mechanisms of action are discussed.

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“…Absorption spectra were taken with a DW-2aUV-Vis spectrophotometer. Oxygen was assayed polarographically, ATP as in [10] and protein as in [11]. For other experimental details see figure and table legends.…”

Section: Methodsmentioning

confidence: 99%

Valinomycin as a tool for the reversal of the effect of protonophoric uncouplers

1982

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Use of the liquid scintillation spectrometer for determining adenosine triphosphate by the luciferase enzyme

Cited by 879 publications

References 10 publications

METABOLISM OF BABESIA PARASITES IN VITRO. GLUCOSE AND ENERGY METABOLISM AND SURVIVAL OF BABESIA RODHAINI IN A BASAL MEDIUM WITH AND WITHOUT ADENOSINE

METABOLISM OF BABESIA PARASITES IN VITRO. GLUCOSE AND ENERGY METABOLISM AND SURVIVAL OF BABESIA RODHAINI IN A BASAL MEDIUM WITH AND WITHOUT ADENOSINE

Copper Deficiency in the Rat. Effect on Adenine Nucleotide Metabolism

Valinomycin as a tool for the reversal of the effect of protonophoric uncouplers

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