1998
DOI: 10.1046/j.1537-2995.1998.38298193101.x
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Use of the gel agglutination technique for determination of fetomaternal hemorrhage

Abstract: The GAT is suited for routine screening. It provides rapid and specific detection of D-positive red cells at clinically relevant concentrations. The test may (rarely) yield false-negative results due to insufficient administration of Rh immune globulin before testing.

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Cited by 15 publications
(21 citation statements)
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“…This critical value of 0.2%, which approximates 5 mL of RBCs, was chosen because massive FMH (≥10 mL RBCs) is extremely rare and even a low dose of 100 µg RhIg can adequately neutralize 5 mL of fetal red cells, which is more common in a sensitized mother. 7,9 Therefore GAT not only estimates FMH, but also can discriminate between FMH levels considered to be critical for giving extra anti-D (ie, 0.2%, 0.3%, and ≥0.4%). 7 Gomez-Arbones and colleagues 5 analyzed 15 blood samples of suspected FMH by both GAT and FC and observed no discordance between these techniques.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…This critical value of 0.2%, which approximates 5 mL of RBCs, was chosen because massive FMH (≥10 mL RBCs) is extremely rare and even a low dose of 100 µg RhIg can adequately neutralize 5 mL of fetal red cells, which is more common in a sensitized mother. 7,9 Therefore GAT not only estimates FMH, but also can discriminate between FMH levels considered to be critical for giving extra anti-D (ie, 0.2%, 0.3%, and ≥0.4%). 7 Gomez-Arbones and colleagues 5 analyzed 15 blood samples of suspected FMH by both GAT and FC and observed no discordance between these techniques.…”
Section: Discussionmentioning
confidence: 99%
“…7,9 Therefore GAT not only estimates FMH, but also can discriminate between FMH levels considered to be critical for giving extra anti-D (ie, 0.2%, 0.3%, and ≥0.4%). 7 Gomez-Arbones and colleagues 5 analyzed 15 blood samples of suspected FMH by both GAT and FC and observed no discordance between these techniques. They documented GAT as a useful method for FMH screening and suggested performing a more accurate technique such as FC when FMH volume is greater.…”
Section: Discussionmentioning
confidence: 99%
“…The cells are then centrifuged, and 50 μl supernatant is tested against indicator cells (0.1% suspension of D positive RBC) on an anti IgG gel card by incubation for 15 min at 37°C. The card is then centrifuged, and the results are interpreted by comparison with the pattern observed with control cells, allowing for different concentrations of 0.1%, 0.2% and 0.4% of Rh(D) positive foetal RBC (Salama et al. , 1996).…”
Section: Methodsmentioning
confidence: 99%
“…The method is applicable for basic rbc serology (blood grouping, antibody screening and differentiation) as well as for special investigations, e.g. direct antiglobulin test, characterization of eluates, Rhesus category cells [2], determination of the amount of immunglobulin classes and subclasses [3,4], and quantification of fetal of rbcs in maternal blood samples [5,6].…”
Section: Discussionmentioning
confidence: 99%