Use of the entomopathogenic fungi Metarhizium anisopliae, Cordyceps bassiana and Isaria fumosorosea to control Diaphorina citri (Hemiptera: Psyllidae) in Persian lime under field conditions

Lezama-Gutiérrez, Roberto; Molina-Ochoa, Jaime; Chávez-Flores, Omar; Ángel-Sahagún, César Andrés; Skoda, S.; Reyes-Martínez, Gerardo; Barba-Reynoso, Marisela; Rebolledo-Domínguez, O.; Ruiz-Aguilar, Graciela M. L.; Foster, John E.

doi:10.1017/s1742758412000069

Cited by 48 publications

(31 citation statements)

References 14 publications

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“…Under semi‐field conditions, Dai et al () observed 62% and 65% mortality of D. citri when treated with 1 × 10 7 conidia/ml of B. bassiana (Bb‐E) and C. fumosorosea (IF‐B) after 7 days of infection, respectively. Lezama‐Gutiérrez et al () determined that the fatality rate of C. fumosorosea , B. bassiana , M. anisopliae to D. citri adults were 22%, 42%, and 50%, respectively, in the field. Compared to these studies and our current findings, the different pathogenicity rates of these fungi may be due to strain distinctions, different biotic, abiotic conditions, including host species, humidity, temperature, and soil type in the various studies (Bugeme, Maniania, Knapp, & Boga, ; Erler & Ates, ; Inyang, Mccartney, & Oyejola, ; Kim, Oh, Yoon, & Sung, ; Sharififard, Mossadegh, & Vazirianzadeh, ).…”

Section: Discussionmentioning

confidence: 99%

“…The utilization of entomopathogenic fungi to control D. citri offers a great development opportunity on account of their significant epidemic potential and the convenience of production. Lezama‐Gutiérrez et al () determined the virulence of three entomopathogenic fungi against D. citri in the field and found 42%, 50%, and 22% mortality of D. citri adults when they infected with Beauveria bassiana , Metarhizium anisopliae, and Cordyceps fumosorosea (Wize) Kepler, B. Shrestha & Spatafora, comb. nov. (MycoBank MB820980), formerly Isaria fumosorosea ， respectively.…”

Section: Introductionmentioning

confidence: 99%

“…The utilization of entomopathogenic fungi to control D. citri offers a great development opportunity on account of their significant epidemic potential and the convenience of production. Lezama-Gutiérrez et al (2012) Kepler et al, 2017), isolated from an adult cadaver of D. citri was identified through morphological as well as phylogenetic data. The pathogenicity of this newly isolated strain to D. citri was analyzed through bioassays against different developmental stages.…”

Section: Introductionmentioning

confidence: 99%

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Identification of a new Cordyceps javanica fungus isolate and its toxicity evaluation against Asian citrus psyllid

Zhang

Guo

et al. 2018

MicrobiologyOpen

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The Asian citrus psyllid, Diaphorina citri Kuwayama, is the most serious pest of citrus worldwide. It acts as a vector for a group of phloem‐limited bacteria ( Candidatus Liberibacter spp.) that causes Huanglongbing (HLB) disease. Thus, D. citri management is an important strategy against HLB, and biological control is currently considered as the most effective method because of the unsustainable and negative side effects of chemical control. Here, we isolated a new strain of entomopathogenic fungus, Cordyceps javanica (GZQ‐1), from one cadaver of D. citri adult based on its morphological and phylogenetic data. Five conidial concentrations of the C. javanica pathogen (1 × 10 3 , 1 × 10 4 , 1 × 10 5 , 1 × 10 6 , and 1 × 10 7 conidia/ml) were assessed against six life stages of D. citri (1st‐5th instar nymphs and adults). Results showed that C. javanica GZQ‐1 was highly pathogenic to D. citri nymphs (69.49%–90.87% mortality) and adults (69.98% mortality). The LC 50 and LT 50 values of C. javanica against 1st‐2nd instar (younger), 3rd‐4th instar (middle aged), 5th instar (older), and adults were 1.20 × 10 5 , 1.10 × 10 6 , 4.47 × 10 6 , 8.12 × 10 6 conidia/ml and 4.25, 4.51, 5.17, 5.49 days, respectively. Moreover, glasshouse experiments indicated that this C. javanica GZQ‐1 caused higher infection rates of D. citri adults compared to two other fungal strains we previously isolated in the laboratory, Cordyceps fumosorosea (IF010) and Metarhizium anisopliae (CNGD7).

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…The utilization of entomopathogenic fungi to control D. citri offers a great development opportunity on account of their significant epidemic potential and the convenience of production. Lezama-Gutiérrez et al (2012) Kepler et al, 2017), isolated from an adult cadaver of D. citri was identified through morphological as well as phylogenetic data. The pathogenicity of this newly isolated strain to D. citri was analyzed through bioassays against different developmental stages.…”

Section: Introductionmentioning

confidence: 99%

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Identification of a new Cordyceps javanica fungus isolate and its toxicity evaluation against Asian citrus psyllid

Zhang

Guo

et al. 2018

MicrobiologyOpen

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“…Further studies are warranted to understand the mechanism underlying decreased susceptibility of cold stress psyllids populations to entomopathogenic fungi. Some studies reported that decrease in susceptibility might influence the management of psyllids under field conditions using I. fumosorosea and H. citriformis (Lezama‐Gutiérrez et al., ; Pérez‐González, Sandoval‐Coronado, & Maldonado‐Blanco, ).…”

Section: Discussionmentioning

confidence: 99%

Susceptibilities of Candidatus Liberibacter asiaticus‐infected and noninfected Diaphorina citri to entomopathogenic fungi and their detoxification enzyme activities under different temperatures

et al. 2018

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Some entomopathogenic fungi species, Isaria fumosorosea, and Hirsutella citriformis were found to be efficient against the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae). However, the susceptibility to these fungi increases when the psyllid infected with Candidatus Liberibacter asiaticus (Las), which is transmitted by D. citri and causes citrus greening disease. In this study, we examined the Las-infected and Las-uninfected D. citri susceptibility to entomopathogenic fungi at different temperature regimes (5-40°C). When D. citri adults exposed to cold temperature (5°C), they showed less susceptibility to entomopathogenic fungi as compared with control (27°C). Irrespective of infection with Las, a significantly positive correlation was observed between temperature and percentage mortality caused by different isolates of I. fumosorosea, 3A Ifr, 5F Ifr, PS Ifr, and H. citriformis isolates, HC3D and 2H. In contrast, a significantly negative correlation was found between temperature and percentage mortality for 3A Ifr for both Las-infected and Las-uninfected psyllids. Detoxification enzymes, Glutathione S-transferase levels in D. citri showed a negative correlation, whereas cytochrome P450 and general esterase levels were not correlated with changes in temperature. These findings revealed that detoxification enzymes and general esterase levels are not correlated with altered susceptibility to entomopathogenic fungi at the different temperature regimes. Conclusively, temperature fluctuations tested appear to be a significant factor impacting the management strategies of D. citri using entomopathogenic fungi.

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“…El uso excesivo de productos químicos en los cultivos agrícolas es un problema, ya que algunos insectos han desarrollado resistencia a estos (Lezama-Gutiérrez et al, 2012). Por lo tanto, se ha vuelto necesario buscar medios alternativos para el control biológico de plagas para poder minimizar el uso de estos químicos (Mirhaghparast et al, 2013).…”

Section: Introductionunclassified

Análisis de las investigaciones sobre Metarhizium anisopliae en los últimos 40 años

HernÃ¡ndez-Rosas

GarcÃa-Pacheco

Figueroa-RodrÃguez

et al. 2019

Remexca

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El control biológico se utiliza para la regulación de poblaciones de insectos plaga y evitar la inducción de resistencia de los insectos por el uso excesivo de agroquímicos, así como solventar problemas de residuos para la producción orgánica de alimentos. Existen más de 750 especies de hongos entomopatógenos que pueden utilizarse para el control biológico de plagas, siendo Metarhizium anisopliae, uno de los más utilizados a nivel comercial. La presente revisión tuvo por objetivo determinar los temas más relevantes con respecto a investigaciones concernientes a M. anisopliae durante el período de 1976 a 2018. Se realizó una revisión en el metabuscador Scopus®, utilizando como palabra clave Metarhizium anisopliae solo en título para octubre 9, 2018. Se identificaron las principales revistas, países e instituciones que han publicado sobre el tema. Los datos se analizaron utilizando el software VOSviewer® bajo el principio de co-ocurrencia de términos. Los resultados muestran que este microorganismo ha sido ampliamente estudiado a nivel internacional, especialmente en el período de 2008 a 2014. Las investigaciones se concentran en tres clústeres: producción-actividad, Beauveria bassiana y termitas. Se concluye que este microorganismo sigue analizándose desde una perspectiva comercial y debido a que los agentes de control biológico tienden a ser locales, sus investigaciones continuarán para concentrarse en el desarrollo y caracterización a nivel molecular de cepas locales (nativas) con potencial comercial.

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Use of the entomopathogenic fungi Metarhizium anisopliae, Cordyceps bassiana and Isaria fumosorosea to control Diaphorina citri (Hemiptera: Psyllidae) in Persian lime under field conditions

Cited by 48 publications

References 14 publications

Identification of a new Cordyceps javanica fungus isolate and its toxicity evaluation against Asian citrus psyllid

Identification of a new Cordyceps javanica fungus isolate and its toxicity evaluation against Asian citrus psyllid

Susceptibilities of Candidatus Liberibacter asiaticus‐infected and noninfected Diaphorina citri to entomopathogenic fungi and their detoxification enzyme activities under different temperatures

Análisis de las investigaciones sobre Metarhizium anisopliae en los últimos 40 años

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