Use of the Ct difference between the Nucleocapside (N) and the Spike (S) or RNA-dependent RNA polymerase (RdRP) genes as a preliminary screening for SARS-CoV-2 variants with the Allplex™ SARS-CoV-2/FluA/FluB/RSV Assay

Urrutikoetxea-Gutierrez, Mikel; Toboso, Ma Carmen Nieto; Zarraga, Estibaliz Ugalde; Aizpurua, Mikele Macho; Arco, José Luis Díaz de Tuesta del

doi:10.1016/j.jviromet.2022.114463

Cited by 3 publications

(3 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, the Omicron sublineage BA.2 (not carrying the del69/70 mutation) does not show S-gene dropout ( 7 ). Thus, as for Alpha or Delta variants ( 9 – 11 ), N-gene dropout, which may be qualified as a shift in the N-gene cycle threshold ( C T ) value compared to other target genes’ values, in multiplex PCR assays could be a valuable indicator of the Omicron variant. Consistently, Omicron variant-defining mutations, such as P13L, E31del, R32del, S33del, R203K, and G204R in the N protein, could lead to N-gene target failure in PCR assays that target the N gene.…”

Section: Observationmentioning

confidence: 99%

Rapid Detection of the Omicron (B.1.1.529) SARS-CoV-2 Variant Using a COVID-19 Diagnostic PCR Assay

et al. 2022

View full text Add to dashboard Cite

show abstract

Section: Observationmentioning

confidence: 99%

Rapid Detection of the Omicron (B.1.1.529) SARS-CoV-2 Variant Using a COVID-19 Diagnostic PCR Assay

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Due to their capability to detect even low viral loads (limit of detection < 1000 copies/mL) [5][6][7], PCR tests have a high diagnostic sensitivity and can identify infections already three days before symptom onset [8]. The amplification of several non-spike gene-targeted sequences in PCR tests assures high sensitivity also for the detection of VoCs carrying numerous spike mutations [9,10]. There are, however, several caveats for the use of PCR tests for SARS-CoV-2 surveillance, among others, the long turnaround time of at least several hours, but often one or two days, in routine diagnostics.…”

Section: Introductionmentioning

confidence: 99%

Vaccine-hesitant individuals accumulate additional COVID-19 risk due to divergent perception and behaviors related to SARS-CoV-2 testing: a population-based, cross-sectional study

Wratil

Kotter²,

Bischof³

et al. 2022

Infection

View full text Add to dashboard Cite

Purpose To investigate the perception of SARS-CoV-2 detection methods, information sources, and opinions on appropriate behavior after receiving negative or positive test results. Methods In a questionnaire-based, cross-sectional study conducted between September 1 and November 17, 2021, epidemiological, behavioral, and COVID-19-related data were acquired from the public in Munich, Germany. Results Most of the 1388 participants obtained information from online media (82.8%) as well as state and federal authorities (80.3%). 93.4% believed in the accuracy of SARS-CoV-2 PCR testing and 41.2% in the accuracy of rapid antigen tests (RATs). However, RATs were preferred for testing (59.1%) over PCR (51.1%). 24.0% of all individuals were willing to ignore hygiene measures and 76.9% were less afraid of SARS-CoV-2 transmission after receiving a negative PCR test (5.9% and 48.8% in case of a negative RAT). 28.8% reported not to self-isolate after receiving a positive RAT. Multivariate analyses revealed that non-vaccinated individuals relied less on information from governmental authorities (p = 0.0004) and more on social media (p = 0.0216), disbelieved in the accuracy of the PCR test (p ≤ 0.0001) while displaying strong preference towards using RATs (p ≤ 0.0001), were more willing to abandon pandemic-related hygiene measures (p ≤ 0.0001), less afraid of transmitting SARS-CoV-2 after a negative RAT (p ≤ 0.0001), and less likely to isolate after a positive RAT (p ≤ 0.0001). Conclusion Insights into preferred information sources as well as perception, preferences, and behavior related to SARS-CoV-2 testing and hygiene measures are key to refining public health information and surveillance campaigns. Non-vaccinated individuals’ divergent believes and behaviors possibly increase their COVID-19 risk.

show abstract

“…Due to its capability to detect even low viral loads (limit of detection < 1,000 copies/mL) [5][6][7], PCR tests have a high diagnostic sensitivity and can identify infections already three days before symptom onset [8]. The ampli cation of several nonspike gene-targeted sequences in PCR tests assures high sensitivity also for the detection of VoCs carrying numerous spike mutations [9,10]. There are, however, several caveats for the use of PCR tests for SARS-CoV-2 surveillance, among others, the long turn-around time of at least several hours, often one or two days, in routine diagnostics.…”

Section: Introductionmentioning

confidence: 99%

Vaccine-hesitant individuals accumulate additional COVID-19 risk due to divergent perception and behaviors related to SARS-CoV-2 testing – a population based, cross-sectional study

Wratil

Kotter

Bischof

et al. 2022

Preprint

View full text Add to dashboard Cite

Purpose To investigate the perception of SARS-CoV-2 detection methods, information sources, and opinions on appropriate behavior after receiving negative or positive test results. Methods In a questionnaire-based, cross-sectional study conducted between September 1 and November 17, 2021, epidemiological, behavioral, and COVID-19-related data was acquired from the public in Munich, Germany. Results Most of the 1,388 participants obtained information from online media (82.8%) as well as state and federal authorities (80.3%). 93.4% believed in the accuracy of SARS-CoV-2 PCR testing and 41.2% in the accuracy of rapid antigen tests (RATs). However, RATs were preferred for testing (59.1%) over PCR (51.1%). 24.0 % of all individuals were willing to ignore hygiene measures and 76.9% were less afraid of SARS-CoV-2 transmission after receiving a negative PCR test (5.9% and 48.8% in case of a negative RAT). 28.8% reported not to self-isolate after receiving a positive RAT. Multivariate analyses revealed that non-vaccinated individuals relied less on information from governmental authorities (p=0.0004) and more on social media (p=0.0216), disbelieved in the accuracy of the PCR test (p≤0.0001) while displaying strong preference towards using RATs (p≤0.0001), were more willing to abandon pandemic-related hygiene measures (p≤0.0001), less afraid of transmitting SARS-CoV-2 after a negative RAT (p≤0.0001), and less likely to isolate after a positive RAT (p≤0.0001). Conclusion Insights into preferred information sources as well as perception, preferences and behavior related to SARS-CoV-2 testing and hygiene measures are key to refining public health information and surveillance campaigns. Non-vaccinated individuals’ divergent believes and behaviors possibly increase their COVID-19 risk.

show abstract

Use of the Ct difference between the Nucleocapside (N) and the Spike (S) or RNA-dependent RNA polymerase (RdRP) genes as a preliminary screening for SARS-CoV-2 variants with the Allplex™ SARS-CoV-2/FluA/FluB/RSV Assay

Abstract: Graphical abstract

Cited by 3 publications

References 12 publications

Rapid Detection of the Omicron (B.1.1.529) SARS-CoV-2 Variant Using a COVID-19 Diagnostic PCR Assay

Rapid Detection of the Omicron (B.1.1.529) SARS-CoV-2 Variant Using a COVID-19 Diagnostic PCR Assay

Vaccine-hesitant individuals accumulate additional COVID-19 risk due to divergent perception and behaviors related to SARS-CoV-2 testing: a population-based, cross-sectional study

Vaccine-hesitant individuals accumulate additional COVID-19 risk due to divergent perception and behaviors related to SARS-CoV-2 testing – a population based, cross-sectional study

Contact Info

Product

Resources

About