Use of the Chromosomal Co-location of the Minor 5S and the Major 28S rDNA as a Cytogenetic Marker within the Genus Leuciscus (Pisces, Cyprinidae)

Abstract

Use of the chromosomal co-location of the minor 5S and the major 28S rDNA as a cytogenetic marker within the genus Leuciscus (Pisces, Cyprinidae). Folia biol. (Kraków) #&: 245-249. The chromosomes of three species from the genus Leuciscus (the ide L. idus, the European chub L. cephalus and the common dace L. leuciscus) were examined with the FISH technique for 5S and 28S rDNA probes. The analysis showed that among the three examined species, 5S rDNA signals were located on two large and four small subtelocentric chromosomes in L. leuciscus, on one large and five small subtelocentric chromosomes in L. idus, while in L. cephalus the probe signals were found on two metacentric chromosomes and one large and one small subtelocentric chromosome pairs. In all analysed species, the 28S rDNA probe signals were placed on only one chromosome pair, subtelocentric in the common dace and the European chub, and submetacentric in the ide. The three species differed in the number of sites in which both probe signals were present. In conclusion, the co-location of the 5S and 28S rDNA proved to be a useful cytogenetic marker among the studied fishes. Moreover, this marker could be adapted to other cyprinids.

Introgressive hybridization as a promoter of genome reshuffling in natural homoploid fish hybrids (Cyprinidae, Leuciscinae)

“…Species-specific rDNA polymorphisms were recently characterized in European leuciscines (Gromicho et al, 2006a,b;Kirtiklis et al, 2010;Pereira et al, 2012;Rossi et al, 2012), as well as in artificially reared hybrids of the Squalius alburnoides complex (Gromicho et al, 2006b). In the present study, each hybrid stood for a distinct rDNA profile, usually falling within the range of possible combinations of parental contributions (Supplementary Table S3) and reflecting their admixed nature.…”

“…The number of studies in cyprinids using dual-FISH with rDNA probes is frankly growing, and colocalized ribosomal genes in leuciscines were described within Anaecypris, Leuciscus and Squalius genera (Gromicho et al, 2006a,b;Kirtiklis et al, 2010;Rossi et al, 2012) but never in chondrostomine species (for example, parental species under focus; Pereira et al, 2012). The novel syntenic associations (Figures 2-4) are distinct evidences of genome instability and reshuffling, most likely driven by the hybridization events (Fontdevila, 2005;Abbott et al, 2013).…”

Comparative cytogenetics of Loricariidae (Actinopterygii: Siluriformes): Emphasis in Neoplecostominae and Hypoptopomatinae

“…FISH using simultaneously 5S and 28S rDNA probes allowed the detection of overlapping hybridization signals in Leuciscus species proved to be a useful cytogenetic marker among cyprinids (Kirtiklis et al 2010). The silver nitrate stained NORs can also be used as efficient markers in Loricariidae.…”

Gene mapping of 28S rDNA sites in allotriploid Cobitis females (Pisces: Cobitidae) from a diploid-polyploid population

“…In many cases, cytogenetic analysis using FISH technique with ribosomal probes is an efficient tool in genome investigation (Alexandre & Martins-Santos 2006;Ross & Peichel 2008;Nicodemus-Johnson et al 2011;Lee et al 2011) and a source of data for fish species identification (Kirtiklis et al 2010). In the present study we have used this technique to find cytogenetical markers of allotriploid Cobitis females useful to compare them with those which characterized the parental species.…”