USE OF THE BRUCELLA IgM AND IgG FLOW ASSAYS IN THE SERODIAGNOSIS OF HUMAN BRUCELLOSIS IN AN AREA ENDEMIC FOR BRUCELLOSIS

Irmak, Hasan; Buzğan, Turan; Omer, Endashaw; Akdeniz, Hayrettin; Demiröz, Ali Pekcan; Abdoel, Theresia H.; Smits, Henk L.

doi:10.4269/ajtmh.2004.70.688

Cited by 45 publications

(45 citation statements)

References 10 publications

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“…This could be an important aspect in countries such as Egypt where the disease is widely spread. Furthermore, our results are in agreement with the high sensitivity and specificity observed for the flow assay by earlier reports from Spain [19], Turkey [21,22], and Kazakhstan [21]. Therefore, in the absence of the laboratory support, the flow assay may be used for the confirmation of brucellosis in febrile patients with clinical suspicion of brucellosis by testing a drop of blood collected by finger prick.…”

Section: Discussionsupporting

confidence: 79%

Laboratory diagnosis of human brucellosis in Egypt and persistence of the pathogen following treatment

Marei

Boghdadi

Abdel-Hamed

et al. 2011

J Infect Dev Ctries

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Introduction: Brucellosis is a major public health problem in Egypt. The Brucella IgM/IgG lateral flow assay was developed as a point-ofcare test for the diagnosis of human brucellosis. The aim of this study was to assess the diagnostic value of the lateral flow assay for use in Egypt. Methodology: Fifty samples of patients who presented with clinical suspicion of brucellosis over a one-year period were collected. All samples were subjected to the Brucella IgM/IgG lateral flow assay, serum agglutination test (SAT), rose bengal RB Test (RB), 2-mercapteoethanol (2-ME), culture and PCR. SAT, 2-ME, culture and PCR were retested after the end of the treatment. Results: Culture and SAT confirmed the diagnosis of brucellosis in twenty patients. While 90% of the samples were positive by SAT, only 30% and 85% were positive by culture and PCR respectively. The sensitivity of the lateral flow assay calculated for the Brucella IgM/IgG was 95% and specificity was 97%. Conclusion: These data show that the lateral flow assay is more suitable for diagnosis of brucellosis in Egypt than culture and SAT. Application of the PCR on serum samples collected during follow-up revealed that the DNA of the pathogen was yet not completely cleared almost 60 days after the start of treatment with doxycycline and ciprofloxacin.

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Section: Discussionsupporting

confidence: 79%

Laboratory diagnosis of human brucellosis in Egypt and persistence of the pathogen following treatment

Marei

Boghdadi

Abdel-Hamed

et al. 2011

J Infect Dev Ctries

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“…Simple, rapid diagnostic tests with adequate performance characteristics on acute specimens are needed to assist with the identification of patients with brucellosis. 8,67,68 To better prevent human brucellosis, it is important to target control of brucellosis in animal reservoirs, 3 including the use of the Brucella species-specific B. melitensis Rev 1 and B. abortus S19 and RB51 vaccines. 69 Closer collaboration between human and animal health experts is needed to better define which Brucella species are responsible for both human and animal brucellosis in sub-Saharan Africa.…”

Section: Discussionmentioning

confidence: 99%

Brucellosis among Hospitalized Febrile Patients in Northern Tanzania

Bouley

Biggs²,

Stoddard³

et al. 2012

The American Journal of Tropical Medicine and Hygiene

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Abstract. Acute and convalescent serum samples were collected from febrile inpatients identified at two hospitals in Moshi, Tanzania. Confirmed brucellosis was defined as a positive blood culture or a 4-fold increase in microagglutination test titer, and probable brucellosis was defined as a single reciprocal titer 160. Among 870 participants enrolled in the study, 455 (52.3%) had paired sera available. Of these, 16 (3.5%) met criteria for confirmed brucellosis. Of 830 participants with 1 serum sample, 4 (0.5%) met criteria for probable brucellosis. Brucellosis was associated with increased median age (P = 0.024), leukopenia (odds ratio [OR] 7.8, P = 0.005), thrombocytopenia (OR 3.9, P = 0.018), and evidence of other zoonoses (OR 3.2, P = 0.026). Brucellosis was never diagnosed clinically, and although all participants with brucellosis received antibacterials or antimalarials in the hospital, no participant received standard brucellosis treatment. Brucellosis is an underdiagnosed and untreated cause of febrile disease among hospitalized adult and pediatric patients in northern Tanzania.

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“…The high incidence of brucellosis in Turkey is due to the widespread infection among domestic animals and the frequent contacts with livestock and the consumption of raw milk and traditionally prepared soft cheeses by the rural population [1]. So, brucellosis and its complications are still a serious public health concern, especially in Eastern Anatolia of Turkey [1,13,18,36]. In the region of Van (located in Eastern Anatolia), which is an area of intensive animal husbandry, brucellosis is common in sheep [16,17].…”

mentioning

confidence: 99%

<i>In Vitro</i> Antimicrobial Susceptibility of <i>Brucella melitensis</i> Isolates from Sheep in an Area Endemic for Human Brucellosis in Turkey

Ilhan

Solmaz

Ekin

2013

The Journal of Veterinary Medical Science

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ABSTRACT. The aim of this study was to assess in vitro antimicrobial susceptibility of Brucella melitensis isolates isolated from naturally infected sheep cases in an area where human brucellosis is endemic, focusing on rifampin (RIF), streptomycin (SM), ciprofloxacin (CPFX), trimethoprim/sulfamethoxazole (TMP/SMZ), gentamicin (GM) and tetracycline (TC) and on 11 other antimicrobials. The identification and typing of Brucella isolates were carried out using standard classification tests and polymerase chain reaction (PCR) methods. Antimicrobial susceptibility testing was carried out on Mueller-Hilton agar. The resistance to SM, CPFX and GM was determined at the rate of 7.3% and to RIF at the rate of 9.7%. The highest (46.3%) resistance was determined against TMP/SMZ. All strains were found to be sensitive to TC at the rate of 100.0%. In conclusion, ovine origin B. melitensis strains evaluated in this study were resistant to at least one antimicrobial (51.2%) that is commonly used in human clinical medicine against brucellosis.

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USE OF THE BRUCELLA IgM AND IgG FLOW ASSAYS IN THE SERODIAGNOSIS OF HUMAN BRUCELLOSIS IN AN AREA ENDEMIC FOR BRUCELLOSIS

Cited by 45 publications

References 10 publications

Laboratory diagnosis of human brucellosis in Egypt and persistence of the pathogen following treatment

Laboratory diagnosis of human brucellosis in Egypt and persistence of the pathogen following treatment

Brucellosis among Hospitalized Febrile Patients in Northern Tanzania

<i>In Vitro</i> Antimicrobial Susceptibility of <i>Brucella melitensis</i> Isolates from Sheep in an Area Endemic for Human Brucellosis in Turkey

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