Use of the avidin (imino)biotin system as a general approach to affinity precipitation

Temperature-dependent intermolecular force measurement of poly(N-isopropylacrylamide) grafted surface with protein

“…It is known that the phase transition of the PNiPAM aqueous solution accompanies not only a drastic conformation change (from a coil to a globule) [1,2], but also a rapid change in interfacial properties [6][7][8][9][10]. This phenomenon makes this polymer useful in drug delivery systems [11], tissue engineering [12,13], and in many other areas [14][15][16].…”

Strategies for the efficient synthesis of stimuli-responsive ss-DNA bioconjugates for capture and transfection of plasmid DNA

“…In cases were the spectra show only one type of polymer with a specific and detectable end group, end group quantification can be used as an alternate means of determining the number average of the molecular weight. Especially in the case of smaller telomers as prepared here, the number average determined by end group titration is usually within 10% of the MALDI mass [33]. However, some caution should be taken in the interpretation of the MALDI MS data keeping this problem in mind.…”

Purification of RT-PCR Competent Poly(A) mRNA from Crude Cell Lysate by Affinity Precipitation

“…Salts and other cosolutes are known to influence the critical solution temperature of an AML (19). The attached affinity ligand, on the other hand, typically has little effect, even in the case of a fairly voluminous molecules (5,12).…”