Use of telemetry to record electrocardiogram and heart rate in freely moving mice

Krämer, K.; Acker, S.A.B.E. van; Voss, Hans–Peter; Grimbergen, J.A.; Vijgh, W.J.F. van der; Bast, Aalt

doi:10.1016/1056-8719(93)90019-b

Cited by 177 publications

(133 citation statements)

References 6 publications

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“…Considering that murine heart rates range from 400 to 800 beats/min (20), an increase in action potential duration of this magnitude could account for LPS-induced bradycardia (22). Projecting in vitro observations to heart function in situ, these findings account at least in part for reduced heart rate variability (8,30,31,38) and depressed cardiac function that accompanies sepsis (36,44).…”

Section: Discussionmentioning

confidence: 99%

Lipopolysaccharide prolongs action potential duration in HL-1 mouse cardiomyocytes

Wondergem

Graves

et al. 2012

American Journal of Physiology-Cell Physiology

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Wondergem R, Graves BM, Li C, Williams DL. Lipopolysaccharide prolongs action potential duration in HL-1 mouse cardiomyocytes. Am J Physiol Cell Physiol 303: C825-C833, 2012. First published August 15, 2012; doi:10.1152/ajpcell.00173.2012.-Sepsis has deleterious effects on cardiac function including reduced contractility. We have shown previously that lipopolysaccharides (LPS) directly affect HL-1 cardiac myocytes by inhibiting Ca 2ϩ regulation and by impairing pacemaker "funny" current, I f. We now explore further cellular mechanisms whereby LPS inhibits excitability in HL-1 cells. LPS (1 g/ml) derived from Salmonella enteritidis decreased rate of firing of spontaneous action potentials in HL-1 cells, and it increased their pacemaker potential durations and decreased their rates of depolarization, all measured by whole cell current clamp. LPS also increased action potential durations and decreased their amplitude in cells paced at 1 Hz with 0.1 nA, and 20 min were necessary for maximal effect. LPS decreased the amplitude of a rapidly inactivating inward current attributed to Na ϩ and of an outward current attributed to K ϩ ; both were measured by whole cell voltage clamp. The K ϩ currents displayed a resurgent outward tail current, which is characteristic of the rapid delayed-rectifier K ϩ current, IKr. LPS accordingly reduced outward currents measured with pipette Cs ϩ substituted for K ϩ to isolate IKr. E-4031 (1 M) markedly inhibited IKr in HL-1 cells and also increased action potential duration; however, the direct effects of E-4031 occurred minutes faster than the slow effects of LPS. We conclude that LPS increases action potential duration in HL-1 mouse cardiomyocytes by inhibition of IKr and decreases their rate of firing by inhibition of INa. This protracted time course points toward an intermediary metabolic event, which either decreases available mouse ether-a-go-go (mERG) and Na ϩ channels or potentiates their inactivation.delayed rectifier K ϩ current; patch-clamp electrophysiology; Salmonella enteritidis; E-4031 SEPSIS IS A COMPLEX CLINICAL problem that can progress into multiorgan dysfunction syndrome (MODS) and death. At least one-half of the high mortality rates of severe-sepsis, sepsis, and MODS result from cardiovascular failure (27). Approximately 40% of patients in septic shock also experience myocardial dysfunction (36, 44), which comprises reduced systolic contractility, impaired diastolic relaxation, and ventricular dilatation. The initial clinical pattern, however, ironically is termed "hyperdynamic," because it often presents with high cardiac output, elevated heart rate, and vasodilatation of the dermis (27). This is attributable in part to the volume loading that is frequently the initial treatment for septic shock patients to maintain cardiac output and organ perfusion (15, 28). As is often the case in the complexity of sepsis, the prevailing symptoms can mask organ and system failure occurring at a deeper level in the clinical spectrum.The most prevalent sign of myocardial depressio...

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Section: Discussionmentioning

confidence: 99%

Lipopolysaccharide prolongs action potential duration in HL-1 mouse cardiomyocytes

Wondergem

Graves

et al. 2012

American Journal of Physiology-Cell Physiology

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“…ECG transmitters (Data Sciences International™, type TA10ETA-F20, St. Paul, MN, USA) were implanted in the abdominal cavity with two electrodes placed subcutaneously as described previously (Kramer et al 1993). One electrode was subcutaneously guided and sutured on the muscle of the lower right chest of the animal, and the other on the muscle bundle of the left shoulder.…”

Section: Surgerymentioning

confidence: 99%

Autonomic Changes Associated with Enhanced Anxiety in 5-HT1A Receptor Knockout Mice

Pattij

Groenink

Hijzen

et al. 2002

Neuropsychopharmacology

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“…Apparently, muscle physiology is not challenged to threshold levels under normal laboratory housing conditions, but we might have missed subtle effects. Therefore, to examine the basic physiological functions in our mutants in somewhat more detail we started computerized telemetric analyses [43,44] of heart beat rate, core temperature and gross locomotor activity. Figure 4 shows the typical recordings of one CK [-/-] double knock out and one wild type animal during a continuous monitoring period of 2 days with successive light (14 h) and dark (10 h) intervals.…”

Section: Physiology and Cytoarchitectural Adaptationsmentioning

confidence: 99%

“…After intraperitoneal surgical implantation of a transmitter (TA10ETA-F20) mice were allowed to recover for 21 days, during which the mice regained their initial, pre-surgical weight. Monitoring was performed during two successive days with light/dark periods of 14/10 h as described [43,44].…”

Section: Physiological Measurements Of Skeletal Muscle Functionmentioning

confidence: 99%

Cytoarchitectural and metabolic adaptations in muscles with mitochondrial and cytosolic creatine kinase deficiencies

Steeghs¹,

Oerlemans²,

Haan³

et al. 1998

Bioenergetics of the Cell: Quantitative Aspects

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Use of telemetry to record electrocardiogram and heart rate in freely moving mice

Cited by 177 publications

References 6 publications

Lipopolysaccharide prolongs action potential duration in HL-1 mouse cardiomyocytes

Lipopolysaccharide prolongs action potential duration in HL-1 mouse cardiomyocytes

Autonomic Changes Associated with Enhanced Anxiety in 5-HT1A Receptor Knockout Mice

Cytoarchitectural and metabolic adaptations in muscles with mitochondrial and cytosolic creatine kinase deficiencies

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