Use of surface-enhanced laser desorption ionization–time-of-flight to identify heat shock protein 70 isoforms in closely related species of the virilis group of Drosophila

Zatsepina, Olga G.; Караванов, А А; Гарбуз, Д. Г.; Shilova, Victoria; Tornatore, Peter; Evgen’ev, Michael B.

doi:10.1379/csc-71.1

Cited by 5 publications

(6 citation statements)

References 17 publications

(19 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All proteins used in these experiments were obtained as gel slices after 2D electrophoresis stained with Coomassie Blue (G‐250). Gel slices were processed exactly as described (Zatsepina et al . 2005).…”

Section: Methodsmentioning

confidence: 99%

Larvae of related Diptera species from thermally contrasting habitats exhibit continuous up‐regulation of heat shock proteins and high thermotolerance

et al. 2008

Self Cite

View full text Add to dashboard Cite

A population of Stratiomys japonica, a species belonging to the family Stratiomyidae (Diptera), common name 'soldier flies', occurs in a hot volcanic spring, which is apparently among the most inhospitable environments for animals because of chemical and thermal conditions. Larvae of this species, which naturally often experience temperatures more than 40 degrees C, have constitutively high concentrations of the normally inducible heat-shock protein Hsp70, but very low level of corresponding mRNA. Larvae of three other species of the same family, Stratiomys singularior, Nemotelus bipunctatus and Oxycera pardalina, are confined to different type semi-aquatic habitats with contrasting thermal regime. However, all of them shared the same pattern of Hsp70 expression. Interestingly, heat-shock treatment of S. japonica larvae activates heat-shock factor and significantly induces Hsp70 synthesis, whereas larvae of O. pardalina, a species from constant cold environment, produce significantly less Hsp70 in response to heat shock. Adults of the four species also exhibit lower, but detectable levels of Hsp70 without heat shock. Larvae of all species studied have very high tolerance to temperature stress in comparison with other Diptera species investigated, probably representing an inherent adaptive feature of all Stratiomyidae enabling successful colonization of highly variable and extreme habitats.

show abstract

Section: Methodsmentioning

confidence: 99%

Larvae of related Diptera species from thermally contrasting habitats exhibit continuous up‐regulation of heat shock proteins and high thermotolerance

et al. 2008

Self Cite

View full text Add to dashboard Cite

show abstract

“…After electrophoresis gels were stained with Coomassie G-250, the gel pieces corresponding to protein spots were excised and processed as previously described (Zatsepina et al, 2005;Garbuz et al, 2008). Protein identification was performed using trypsin fingerprinting and surfaceenhanced laser desorption ionization-time-of-flight-mass spectrometry (MALDI-ToF) followed by a National Center for Biotechnology Information database search using the Profound search engine.…”

Section: Immunoblottingmentioning

confidence: 99%

“…2D electrophoresis of proteins isolated from Dr. melanogaster transformants and determination of the S. singularior Hsp70 protein using peptide fingerprinting Proteins isolated from larvae and adults of the transformed Dr. melanogaster strains were separated using two-dimensional electrophoresis as previously described (O'Farrell et al 1975;Ulmasov et al, 1992). After electrophoresis gels were stained with Coomassie G-250, the gel pieces corresponding to protein spots were excised and processed as previously described (Zatsepina et al, 2005;Garbuz et al, 2008). Protein identification was performed using trypsin fingerprinting and surfaceenhanced laser desorption ionization-time-of-flight-mass spectrometry (MALDI-ToF) followed by a National Center for Biotechnology Information database search using the Profound search engine.…”

Section: Immunoblottingmentioning

confidence: 99%

A Drosophila heat shock response represents an exception rather than a rule amongst Diptera species

Zatsepina

Пржиборо

Yushenova

et al. 2016

Insect Molecular Biology

View full text Add to dashboard Cite

Heat shock protein 70 (Hsp70) is the major player that underlies adaptive response to hyperthermia in all organisms studied to date. We investigated patterns of Hsp70 expression in larvae of dipteran species collected from natural populations of species belonging to four families from different evolutionary lineages of the order Diptera: Stratiomyidae, Tabanidae, Chironomidae and Ceratopogonidae. All investigated species showed a Hsp70 expression pattern that was different from the pattern in Drosophila. In contrast to Drosophila, all of the species in the families studied were characterized by high constitutive levels of Hsp70, which was more stable than that in Drosophila. When Stratiomyidae Hsp70 proteins were expressed in Drosophila cells, they became as short-lived as the endogenous Hsp70. Interestingly, three species of Ceratopogonidae and a cold-water species of Chironomidae exhibited high constitutive levels of Hsp70 mRNA and high basal levels of Hsp70. Furthermore, two species of Tabanidae were characterized by significant constitutive levels of Hsp70 and highly stable Hsp70 mRNA. In most cases, heat-resistant species were characterized by a higher basal level of Hsp70 than more thermosensitive species. These data suggest that different trends were realized during the evolution of the molecular mechanisms underlying the regulation of the responses of Hsp70 genes to temperature fluctuations in the studied families.

show abstract

“…It is well known that in different organisms the various groups of Hsps are represented by multiple isoforms and sometimes it is diffi cult to say whether they are encoded by different genes or just result from post-translational modifi cations of the same protein (Norris et al 1995;Zatsepina et al 2005;Tomanek 2005).…”

Section: Different Hsps Exhibit Maximal Synthesis Depending On the Sementioning

confidence: 99%

Molecular mechanisms underlying thermal adaptation of xeric animals

et al. 2007

View full text Add to dashboard Cite

Use of surface-enhanced laser desorption ionization–time-of-flight to identify heat shock protein 70 isoforms in closely related species of the virilis group of Drosophila

Cited by 5 publications

References 17 publications

Larvae of related Diptera species from thermally contrasting habitats exhibit continuous up‐regulation of heat shock proteins and high thermotolerance

Larvae of related Diptera species from thermally contrasting habitats exhibit continuous up‐regulation of heat shock proteins and high thermotolerance

A Drosophila heat shock response represents an exception rather than a rule amongst Diptera species

Molecular mechanisms underlying thermal adaptation of xeric animals

Contact Info

Product

Resources

About