Use of ^99mTc-mononuclear leukocyte scintigraphy in nosocomial fever

Abstract

Mononuclear leukocyte 99mTc scintigraphy showed high sensitivity, specificity, positive and negative predictive values in patients with nosocomial FUO. These results suggest an important role for nuclear medicine in the management of patients with infection/inflammation.

Biodistribution of bone marrow mononuclear cells in chronic chagasic cardiomyopathy after intracoronary injection

“…It has been used for visualization of infection, osteomyelitis, graft rejection and fever of unknown origin [11][12][13][14]. When compared to techniques such as 99m Tc-hexamethyl propylene-amine oxime ( 99m Tc-HMPAO) labeling, there are advantages such as smaller amount of blood required, shorter performing time and lower cost [12][13][14][15]. Based on this experience, we have developed a new and simple technique for labeling BM MNCs and BM MSCs with 99m Tc [16][17][18].…”

“…The 6-hour half-life of 99m Tc allows the monitoring of cell distribution for approximately 24 h, which is an important advantage over the half-life of 110 min of 18F-fluorodeoxyglycose (FDG). In comparison with indium-111 oxine, another commonly used radiopharmaceutical, 99m Tc results in better image resolution and a lower radiation burden to the patient [12][13][14][15]32].…”

“…After washing, cells were resuspended in 20 ml of saline solution with 5% autologous serum. Five ml (25%) of this solution was used for labeling with 99m Tc as previously described [12][13][14][15][16][17][18]. In short, a saline solution of SnCl2 was added to the cell suspension in 0.9% NaCl and the mixture incubated at room temperature for 10 min.…”

Radiopharmaceutical Stem Cell Tracking for Neurological Diseases

“…Radiopharmaceutical cell labeling has been used for decades to systemically monitor cells in nuclear medicine studies such as labeled leukocyte scintigraphy for detection of infectious and inflammatory diseases [5–7]. Technetium-99m (  99m Tc) is currently the most used radionuclide in the world and is imaged with conventional nuclear medicine techniques, that is, 2-dimensional planar scans or 3-dimensional single photon emission computed tomography (SPECT).…”

“…Additionally, SPECT images may be fused with conventional computed tomography (CT), resulting in SPECT/CT images that allow attenuation correction and better localization of nuclear medicine findings, significantly improving both sensitivity and specificity [8]. 99m Tc has wider availability and lower cost than other radionuclides, and its 6-h physical half-life allows cell tracking for up to 24 h with good resolution and low radiation dose to the patient and to the labeled cells [5–7]. …”