Use of<sup>13</sup>C-labeled Glucose for Measuring Exogenous Glucose Oxidation in Mice

Ishihara, Kengo; Oyaizu, Shinichi; Mizunoya, Wataru; Fukuchi, Yoshiko; Yasumoto, Kyoden; Fushiki, Tohru

doi:10.1271/bbb.66.426

Cited by 21 publications

(12 citation statements)

References 9 publications

(9 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By supplementing an animal's diet with known amounts of 13 C-labeled molecules one can investigate the time course over which different exogenous physiological fuels are oxidized to meet an animal's energy demands -a procedure known as 'breath testing'. Breath testing, using orally or intravenously administered stable isotope tracers, has been regularly used for two decades to diagnose infections, malabsorption and metabolic disorders in humans (Amarri and Weaver, 1995;Bodamer and Halliday, 2001;Wetzel, 2005), but the growing number of commercially available tracer molecules (Amarri and Weaver, 1995;Bodamer and Halliday, 2001;Ishihara et al, 2002) and the reduced costs and high throughput of continuous-flow isotope-ratio mass spectrometers (Martinez del Rio et al, 2009;McCue, 2008) makes breath testing increasingly available to comparative physiologists (Hatch et al, 2002a;McCue and Pollock, 2008).…”

Section: Introductionmentioning

confidence: 99%

Tracking the oxidative kinetics of carbohydrates, amino acids and fatty acids in the house sparrow using exhaled 13CO2

McCue

Sivan

McWilliams

et al. 2010

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARYClinicians commonly measure the 13 CO 2 in exhaled breath samples following administration of a metabolic tracer (breath testing) to diagnose certain infections and metabolic disorders. We believe that breath testing can become a powerful tool to investigate novel questions about the influence of ecological and physiological factors on the oxidative fates of exogenous nutrients. Here we examined several predictions regarding the oxidative kinetics of specific carbohydrates, amino acids and fatty acids in a dietary generalist, the house sparrow (Passer domesticus). After administering postprandial birds with 20mg of one of seven 13 Clabeled tracers, we measured rates of 13 CO 2 production every 15min over 2h. We found that sparrows oxidized exogenous amino acids far more rapidly than carbohydrates or fatty acids, and that different tracers belonging to the same class of physiological fuels had unique oxidative kinetics. Glycine had a mean maximum rate of oxidation (2021nmolmin ), and differed significantly in the extent to which each was oxidized, with 0.73%, 0.63% and 0.21% of palmitic, oleic and stearic acid tracers oxidized, respectively.

show abstract

Section: Introductionmentioning

confidence: 99%

Tracking the oxidative kinetics of carbohydrates, amino acids and fatty acids in the house sparrow using exhaled 13CO2

McCue

Sivan

McWilliams

et al. 2010

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…Gas analysis was performed using an open-circuit metabolic gas analysis system connected directly to a mass spectrometer (model Arco2000; ArcoSystem, Chiba, Japan). The gas analysis system is described in detail elsewhere (28,29). · Briefly, each metabolic chamber had a 72-cm 2 floor and was 6 cm in height.…”

Section: Methodsmentioning

confidence: 99%

Intragastric Administration of Allyl Isothiocyanate Reduces Hyperglycemia in Intraperitoneal Glucose Tolerance Test (IPGTT) by Enhancing Blood Glucose Consumption in Mice

Mori

Kurata

Yamazaki

et al. 2013

J Nutr Sci Vitaminol

Self Cite

View full text Add to dashboard Cite

56Controlling glucose metabolism is important to prevent the development of metabolic disorders such as obesity and type 2 diabetes. Recent studies revealed that postprandial dysmetabolism (i.e., hyperglycemia and hyperlipidemia) is related to the risk of developing obesity and cardiovascular disease (1-4). Postprandial hyperglycemia and hyperlipidaemia induce endothelial dysfunction associated with increased oxidative stress and vascular inflammation, and are involved in the pathogenesis of atherosclerosis. Therefore, it is important to control postprandial metabolic states and reduce postprandial metabolic abnormalities.The transient receptor potential (TRP) channel family is composed of a wide variety of cation-permeable channels and shows great diversity in its mechanisms of activation. TRPV1 and TRPA1 are cation channels belonging to the TRP channel family, and are activated by high (5, 6) and low (7, 8) nociceptive temperatures, respectively. Interestingly, they are also activated by spicy or pungent compounds in foods, such as capsaicin, piperine, cinnamaldehyde and allicin (5, 7, 9, 10). Recent studies indicate that the activation of TRPV1 or TRPA1 is involved not only in nociception and thermosensation but also thermoregulation and energy metabolisms (11-15).Allyl isothiocyanate (AITC) is a natural compound in plants belonging to the family Cruciferae, and is the pungent ingredient in mustard, horseradish and wasabi. AITC activates TRPA1 (16), and recent studies suggest that it could activate not only TRPA1 but also TRPV1 (17,18).Research has suggested that TRPV1 is involved in the emergence of diabetes and obesity (19)(20)(21)(22). Long-term treatment with TRPV1 antagonists reduces fasting glucose, triglyceride, and insulin levels in ob/ob mice (21). Moreover, dietary capsaicin, which is a TRPV1 agonist, is expected to improve not only obesity-induced inflammation but also obesity-related metabolic disorders such as insulin resistance (20). We previously demonstrated that intragastric administration of AITC increases carbohydrate oxidation via TRPV1 (18). Based on these facts, TRPV1 is considered to play a key role in glucose metabolism.Approaches for suppressing postprandial hyperglycemia have been studied, but that research was generally aimed at preventing glucose absorption by inhibiting digestive enzymes (23-25). We considered that the increase in carbohydrate oxidation by administering Summary We investigated the effects of allyl isothiocyanate (AITC) on the blood glucose levels of mice using an intraperitoneal glucose tolerance test. The intragastric administration of 25 mg/kg body weight AITC reduced the increase in blood glucose level after 2 g/ kg body weight glucose was given intraperitoneally, compared with that of control mice. Intragastric Administration of Allyl Isothiocyanate Reduces Hyperglycemia in Intraperitoneal Glucose Tolerance Test (IPGTT) by Enhancing Blood Glucose Consumption in MiceTo elucidate the mechanism responsible for the reduction, respiratory gas analysis employing 13...

show abstract

“…Gas analysis was performed using an open-circuit metabolic gas analysis system connected directly to a mass spectrometer (model Arco2000; ArcoSystem, Chiba, Japan). The gas analysis system has been described in detail elsewhere (17,18). Briefly, each metabolic chamber had a 72-cm 2 floor and was 6 cm in height.…”

Section: Methodsmentioning

confidence: 99%

“…13 C-labeled oleic acid was added in quantities calculated to result in a concentration similar to that recommended by Ishihara et al (17) as follows. A solution containing 0.3% methylcellulose (emulsifier), 10% corn oil (Wako), and 0.02 mol/l of 13 Clabeled oleic acid (Isotec, Tokyo, Japan) was administered to mice intragastrically (0.01 ml/g of body mass) 30 min after intraperitoneal mercaptoacetate administration.…”

Section: Methodsmentioning

confidence: 99%

Mercaptoacetate inhibition of fatty acid β-oxidation attenuates the oral acceptance of fat in BALB/c mice

Matsumura

Saitou²,

Miyaki³

et al. 2008

American Journal of Physiology-Regulatory, Integrative and Comp

Self Cite

View full text Add to dashboard Cite

We investigated the effect of beta-oxidation inhibition on the fat ingestive behavior of BALB/c mice. Intraperitoneal administration to mice of mercaptoacetate, an inhibitor of fatty acid oxidation, significantly suppressed intake of corn oil but not intake of sucrose solution or laboratory chow. To further examine the effect of mercaptoacetate on the acceptability of corn oil in the oral cavity, we examined short-term licking behavior. Mercaptoacetate significantly and specifically decreased the number of licks of corn oil within a 60-s period but did not affect those of a sucrose solution, a monosodium glutamate solution, or mineral oil. In contrast, the administration of 2-deoxyglucose, an inhibitor of glucose metabolism, did not affect the intake or short-term licking counts of any of the tasted solutions. These findings suggest that fat metabolism is involved in the mechanism underlying the oral acceptance of fat as an energy source.

show abstract

Use of¹³C-labeled Glucose for Measuring Exogenous Glucose Oxidation in Mice

Cited by 21 publications

References 9 publications

Tracking the oxidative kinetics of carbohydrates, amino acids and fatty acids in the house sparrow using exhaled 13CO2

Tracking the oxidative kinetics of carbohydrates, amino acids and fatty acids in the house sparrow using exhaled 13CO2

Intragastric Administration of Allyl Isothiocyanate Reduces Hyperglycemia in Intraperitoneal Glucose Tolerance Test (IPGTT) by Enhancing Blood Glucose Consumption in Mice

Mercaptoacetate inhibition of fatty acid β-oxidation attenuates the oral acceptance of fat in BALB/c mice

Contact Info

Product

Resources

About

Use of13C-labeled Glucose for Measuring Exogenous Glucose Oxidation in Mice

Cited by 21 publications

References 9 publications

Tracking the oxidative kinetics of carbohydrates, amino acids and fatty acids in the house sparrow using exhaled 13CO2

Tracking the oxidative kinetics of carbohydrates, amino acids and fatty acids in the house sparrow using exhaled 13CO2

Intragastric Administration of Allyl Isothiocyanate Reduces Hyperglycemia in Intraperitoneal Glucose Tolerance Test (IPGTT) by Enhancing Blood Glucose Consumption in Mice

Mercaptoacetate inhibition of fatty acid β-oxidation attenuates the oral acceptance of fat in BALB/c mice

Contact Info

Product

Resources

About

Use of¹³C-labeled Glucose for Measuring Exogenous Glucose Oxidation in Mice