Use of sulphopropyl-sephadex chromatography and disc gel electrophoresis for the fractionation and identification of Elapid venoms

Chatman, Virginia B.; DiMari, Samuel J.

doi:10.1016/0041-0101(74)90008-7

Cited by 11 publications

(1 citation statement)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…N. siamensis venom was obtained from Biologicals Unlimited (Baltimore, Md., U.S.A.). The major ~-neurotoxin of this venom was isolated by a slight modification (Miller et aL, 1977) of the chromatographic method of Chatman & DiMari (1974). The purity of each preparation was established by polyacrylamide gel electrophoresis at both high and low pH (Gabriel, 1972;Bonner et aL, 1968), and in the presence of SDS (Weber & Osborn, 1969).…”

Section: Methodsmentioning

confidence: 99%

Inhibition of Herpes Simplex Virus Replication by Cobra -Neurotoxoid

Yourist

Haines

Miller

1983

Journal of General Virology

View full text Add to dashboard Cite

SUMMARYA peptide, prepared by gentle oxidative detoxification of a methionine-free cobra c~-neurotoxin, was evaluated for antiviral activities in vivo and in vitro. When added to BHK cells before infection the peptide reduced herpes simplex virus type 1 (HSV-1)-induced TCDs0 in a dose-and time-dependent manner. The antiviral effect was also expressed on initiation of cell treatment 1 h after infection if the peptide was left in contact with cells. Single, intracerebral doses of the toxoid in suckling mice significantly increased 50~ survival times, and reduced virus replication in the infected brain tissues. Cutaneous lesions and resultant scar formation induced by HSV-1 in hairless mice were also significantly reduced by subcutaneous peptide injections.

show abstract