Use of Streptococcus thermophilus for the in situ production of γ-aminobutyric acid-enriched fermented milk

Han, Mei; Liao, Wenyan; Wu, Shen-mao; Gong, Xia; Chen, Bai

doi:10.3168/jds.2019-16856

Cited by 36 publications

(23 citation statements)

References 31 publications

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“…One of the questions raised by this study is whether these Lactobacillus themselves are capable of secreting feruloyl esterase. Lactobacillus strains have been used as probiotics in fermented food to improve human health ( Han et al, 2020 ). Those capable of producing feruloyl esterase have greater potential for application.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Enzyme Secretion and Ferulic Acid Production by Escherichia coli Expressing Different Lactobacillus Feruloyl Esterases

Kong

Zhang

et al. 2020

Front. Microbiol.

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Construction of recombinant Escherichia coli strains carrying feruloyl esterase genes for secretory expression offers an attractive way to facilitate enzyme purification and one-step production of ferulic acid from agricultural waste. A total of 10 feruloyl esterases derived from nine Lactobacillus species were expressed in E. coli BL21 (DE3) to investigate their secretion and ferulic acid production. Extracellular activity determination showed all these Lactobacillus feruloyl esterases could be secreted out of E. coli cells. However, protein analysis indicated that they could be classified as three types. The first type presented a low secretion level, including feruloyl esterases derived from Lactobacillus acidophilus and Lactobacillus johnsonii. The second type showed a high secretion level, including feruloyl esterases derived from Lactobacillus amylovorus, Lactobacillus crispatus, Lactobacillus gasseri, and Lactobacillus helveticus. The third type also behaved a high secretion level but easy degradation, including feruloyl esterases derived from Lactobacillus farciminis, Lactobacillus fermentum, and Lactobacillus reuteri. Moreover, these recombinant E. coli strains could directly release ferulic acid from agricultural waste. The highest yield was 140 μg on the basis of 0.1 g de-starched wheat bran by using E. coli expressed L. amylovorus feruloyl esterase. These results provided a solid basis for the production of feruloyl esterase and ferulic acid.

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Section: Discussionmentioning

confidence: 99%

Comparison of Enzyme Secretion and Ferulic Acid Production by Escherichia coli Expressing Different Lactobacillus Feruloyl Esterases

Kong

Zhang

et al. 2020

Front. Microbiol.

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“…One of the questions raised by this study is whether these Lactobacillus themselves are capable of secreting feruloyl esterase. Lactobacillus strains as probiotics have been used in fermented food to improve human health [29]. Those capable of producing feruloyl esterase show greater potential for application.…”

Section: Discussionmentioning

confidence: 99%

The commonness and difference among the Lactobacillus feruloyl esterases expressed in Escherichia coli

Xu¹,

Kong²,

Zhang³

et al. 2020

Preprint

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Background: Construction of recombinant Escherichia coli strains carrying feruloyl esterase genes for secretory expression offers an attractive way to facilitate enzyme purification and one-step produce ferulic acid from agricultural waste. It was found that the Lactobacillus crispatus feruloyl esterase could be secreted into extracellular environment of E. coli. Whether other Lactobacillus feruloyl esterases share the same secretory characteristic is worth investigation.Results: A total of ten feruloyl esterases derived from nine Lactobacillus species were used to analyze their commonness and compare their difference when heterologously expressed in E. coli BL21 (DE3). Extracellular activity determination showed all these Lactobacillus feruloyl esterases could be secreted out of E. coli cells. However, protein analysis indicated that they could be classified as three types. The first type presented a low secretion level, including feruloyl esterases derived from Lb. acidophilus and Lb. johnsonii. The second type showed a high secretion level, including feruloyl esterases derived from Lb. amylovorus, Lb. crispatus, Lb. gasseri and Lb. helveticus. The third type also behaved a high secretion level but easy degradation, including feruloyl esterases derived from Lb. farciminis, Lb. fermentum and Lb. reuteri. Moreover, these recombinant E. coli strains could directly release ferulic acid from de-starched wheat bran. Conclusions: Recombinant E. coli strains expressing feruloyl esterase of Lb. amylovorus, Lb. crispatus and Lb. helveticus displayed high secretion level and stable extracellular activity. Furthermore, the highest yield of ferulic acid was 140 µg on the basis of 0.1 g de-starched wheat bran after 72 h cultivation of E. coli expressing Lb. amylovorus feruloyl esterase. These results provided a solid basis for the production of feruloyl esterase and ferulic acid.

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“…Few fermentation methods for the bioproduction have been tested and proposed [20][21][22][23][24][25]. Some of these have reported the use of immobilized microbial cells, batch fermentation, and sourdough fermentation.…”

Section: Strategies For Biosynthesismentioning

confidence: 99%

An Overview of Bioprocesses Employing Specifically Selected Microbial Catalysts for γ-Aminobutyric Acid Production

Dahiya

Manuel

Nigam³

2021

Microorganisms

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Gamma-aminobutyric acid (GABA) is an important chemical compound in the human brain. GABA acts as an inhibitory neurotransmitter by inducing hyperpolarization of cellular membranes. Usually, this pharmaceutically important compound is synthesized using a chemical process, but in this short overview we have only analysed microbial processes, which have been studied for the biosynthesis of this commercially important compound. The content of this article includes the following summarised information: the search for biological processes showed a number of lactic acid bacteria and certain species of fungi, which could be effectively used for the production of GABA. Strains found to possess GABA-producing pathways include Lactobacillus brevis CRL 1942, L. plantarum FNCC 260, Streptococcus salivarius subsp. thermophilus Y2, Bifidobacterium strains, Monascus spp., and Rhizopus spp. Each of these strains required specific growth conditions. However, several factors were common among these strains, such as the use of two main supplements in their fermentation medium—monosodium glutamate and pyridoxal phosphate—and maintaining an acidic pH. Optimization studies of GABA production were comprised of altering the media constituents, modifying growth conditions, types of cultivation system, and genetic manipulation. Some strains increased the production of GABA under anaerobic conditions. Genetic manipulation focused on silencing some genes or overexpression of gadB and gadC. The conclusion, based on the review of information available in published research, is that the targeted manipulation of selected microorganisms, as well as the culture conditions for an optimised bioprocess, should be adopted for an increased production of GABA to meet its increasing demand for food and pharmaceutical applications.

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Use of Streptococcus thermophilus for the in situ production of γ-aminobutyric acid-enriched fermented milk

Cited by 36 publications

References 31 publications

Comparison of Enzyme Secretion and Ferulic Acid Production by Escherichia coli Expressing Different Lactobacillus Feruloyl Esterases

Comparison of Enzyme Secretion and Ferulic Acid Production by Escherichia coli Expressing Different Lactobacillus Feruloyl Esterases

The commonness and difference among the Lactobacillus feruloyl esterases expressed in Escherichia coli

An Overview of Bioprocesses Employing Specifically Selected Microbial Catalysts for γ-Aminobutyric Acid Production

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