Use of stem-cell sheets expressing bone morphogenetic protein-7 in the management of a nonunion radial fracture in a Toy Poodle

Song, Jaeyong; Kim, Yongsun; Kweon, Oh‐Kyeong; Kang, Byung‐Jae

doi:10.4142/jvs.2017.18.4.555

Cited by 11 publications

(9 citation statements)

References 14 publications

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“…Growth factors as FGF-2, TGF-β combined with ADSC on a polysaccharide-based tissue engineered periosteum have shown promising results as they promote a more stable harvesting and integration in murine models [ 41 ]. Furthermore, different studies proved the increased osteogenic properties of transgenic ADSCs overexpressing bone morphogenetic protein 2 (BMP2) [ 42 ] or BMP-7 [ 43 ] in promoting bone healing of long-bones defects. Nonetheless, despite the high transfection efficiency and yield in the transgene expression that they enable, the use of adenoviral vectors for gene transfer seems to complicate a rapid translation of such protocols in the clinical practice.…”

Section: Discussionmentioning

confidence: 99%

Clinical Application of Adipose Derived Stem Cells for the Treatment of Aseptic Non-Unions: Current Stage and Future Perspectives—Systematic Review

Smakaj

Mauro

Rovere

et al. 2022

IJMS

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Fracture non-union is a challenging orthopaedic issue and a socio-economic global burden. Several biological therapies have been introduced to improve traditional surgical approaches. Among these, the latest research has been focusing on adipose tissue as a powerful source of mesenchymal stromal cells, namely, adipose-derived stem cells (ADSCs). ADSC are commonly isolated from the stromal vascular fraction (SVF) of liposuctioned hypodermal adipose tissue, and their applications have been widely investigated in many fields, including non-union fractures among musculoskeletal disorders. This review aims at providing a comprehensive update of the literature on clinical application of ADSCs for the treatment of non-unions in humans. The study was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). Only three articles met our inclusion criteria, with a total of 12 cases analyzed for demographics and harvesting, potential manufacturing and implantation of ADSCs. The review of the literature suggests that adipose derived cell therapy can represent a promising alternative in bone regenerative medicine for the enhancement of non-unions and bone defects. The low number of manuscripts reporting ADSC-based therapies for long bone fracture healing suggests some critical issues that are discussed in this review. Nevertheless, further investigations on human ADSC therapies are needed to improve the knowledge on their translational potential and to possibly achieve a consensus on their use for such applications.

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Section: Discussionmentioning

confidence: 99%

Clinical Application of Adipose Derived Stem Cells for the Treatment of Aseptic Non-Unions: Current Stage and Future Perspectives—Systematic Review

Smakaj

Mauro

Rovere

et al. 2022

IJMS

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“…BMP-7 expressing stem-cell sheets were applied in the previous studies (20–22). In a previous report, BMP-7 overexpressing adenovirus vector was used to transfect mesenchymal stem cells (22).…”

Section: Discussionmentioning

confidence: 99%

“…In a previous report, BMP-7 overexpressing adenovirus vector was used to transfect mesenchymal stem cells (22). In a non-union fracture model, BMP-7 expressing mesenchymal stem cells enhanced bone regeneration (20). Similarly, BMP-7 expressing stem cells produced enhanced bone healing in critical-sized defects when compared with the control stem cell sheets (23).…”

Section: Discussionmentioning

confidence: 99%

Bone morphogenetic protein‑7 upregulates genes associated with osteoblast differentiation, including collagen I, Sp7 and IBSP in gingiva‑derived stem cells

et al. 2019

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The present study was performed to evaluate the effects of short-term application of bone morphogenetic protein-7 (BMP-7) on human gingiva-derived mesenchymal stem cells with next-generation sequencing. Human gingiva-derived stem cells were treated with a final concentration of 100 ng/ml BMP-7 and the same concentration of a vehicle control. mRNA sequencing and data analysis were performed along using gene ontology and pathway analysis. RT-qPCR of mRNA of collagen I, Sp7, IBSP and western blot analysis of collagen I, osterix and bone sialoprotein was also performed. A total of 25,737 mRNAs were identified to be differentially expressed. Regarding osteoblast differentiation, 14 mRNAs were upregulated and 10 were downregulated when the results of the BMP-7 at 3 h were compared with the control at 3 h. The expression of collagen I was increased following the application of BMP-7 at 3 h, and this increase was also observed following western blot analysis. The effects of BMP-7 on stem cells were evaluated with mRNA sequencing, and the expression was validated with RT-qPCR and western blot analysis. The short-term application of BMP-7 produced an increased expression of collagen I, which was associated with target genes selected for osteoblast differentiation. This study may provide novel insights into the role of BMP-7 using mRNA sequencing.

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“…In addition, concerns that BMP treatment does not return bone to normal form and function after remodelling have been reported. 9 In veterinary medicine, the use of BMP has been successful in the treatment of nonunion long bone fractures, [10][11][12][13][14][15] mandibular reconstruction [16][17][18][19] and arthrodesis. [20][21][22] Massie and colleagues reported the use of compression-resistant matrix (CRM) infused with recombinant human bone morphogenetic protein-2 (rhBMP-2) in dogs and the short-term study demonstrated that rhBMP-2 is safe and effective, with 9 of 11 treated fractures returning to full function and the remaining 2 fractures having acceptable function.…”

Section: Introductionmentioning

confidence: 99%

Long-Term Assessment of Bone Regeneration in Nonunion Fractures Treated with Compression-Resistant Matrix and Recombinant Human Bone Morphogenetic Protein-2 in Dogs

Castilla

Filliquist

Spriet

et al. 2022

Vet Comp Orthop Traumatol

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Objective The aim of this study was to assess bone density, bone architecture and clinical function of canine nonunion distal appendicular long bone fractures with a defect treated with fixation, compression-resistant matrix and recombinant human bone morphogenetic protein-2 (rhBMP-2). Study Design Prospective cohort study with dogs at least 1-year post treatment. Computed tomography was performed and quantitative measurements from previous fracture sites were compared with measurements from contralateral limbs. Subjective evaluation included gait assessment and palpation. Results Six patients met the inclusion criteria. The rhBMP-2 treated bone exhibited higher density at the periphery and lower density in the centre, similar to the contralateral limb. All patients were weight bearing on the treated limb and all fractures were healed. Conclusion The rhBMP-2-treated bone underwent restoration of normal architecture and density. Acceptable limb function was present in all patients. The results of this study can serve as a basis for long-term response in treating nonunion fractures in veterinary patients.

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Use of stem-cell sheets expressing bone morphogenetic protein-7 in the management of a nonunion radial fracture in a Toy Poodle

Cited by 11 publications

References 14 publications

Clinical Application of Adipose Derived Stem Cells for the Treatment of Aseptic Non-Unions: Current Stage and Future Perspectives—Systematic Review

Clinical Application of Adipose Derived Stem Cells for the Treatment of Aseptic Non-Unions: Current Stage and Future Perspectives—Systematic Review

Bone morphogenetic protein‑7 upregulates genes associated with osteoblast differentiation, including collagen I, Sp7 and IBSP in gingiva‑derived stem cells

Long-Term Assessment of Bone Regeneration in Nonunion Fractures Treated with Compression-Resistant Matrix and Recombinant Human Bone Morphogenetic Protein-2 in Dogs

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