Use of staining and inhibitors to separate fungal and bacterial activity in soil

Stamatiadis, Stamatis; Doran, John W.; Ingham, E. R.

doi:10.1016/0038-0717(90)90064-7

Cited by 73 publications

(28 citation statements)

References 19 publications

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“…Three slides were evaluated from each sample and ten fields per slide were evaluated with phase contrast microscopy for total hyphal length, and three transects were evaluated for FDAstained (active) hyphal length at 160 total magnification. Iodonitrotetrazolium (INT) stain was used for counting active bacteria (Stadmatiadis et al, 1990). A 1 ml sample of initial soil suspension was diluted to a final dilution in a 0.2 mg soil in 4 ml buffer.…”

Section: Active Bacterial and Fungal Biomassmentioning

confidence: 99%

Influence of irrigated agriculture on soil microbial diversity

Entry

Mills

Mathee

et al. 2008

Applied Soil Ecology

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Section: Active Bacterial and Fungal Biomassmentioning

confidence: 99%

Influence of irrigated agriculture on soil microbial diversity

Entry

Mills

Mathee

et al. 2008

Applied Soil Ecology

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“…The sample was mixed and an aliquot placed on a microscope slide containing a cavity of known volume (Ingham and Klein 1984). Slides were examined for FDA-stained hyphal length immediately after preparation by epifluorescent microscopy (Stamatiadis et al 1990). Three fields slide' were evaluated with phase contrast microscopy for total hyphal length.…”

Section: Experimental Designmentioning

confidence: 99%

Influence of nitrogen on cellulose and lignin mineralization in blackwater and redwater forested wetland soils

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2000

Biology and Fertility of Soils

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Microcosms were used to determine the influence of N additions on active bacterial and active fungal biomass, cellulose degradation and lignin degradation at 5, 10 and 15 weeks in soils from blackwater and redwater wetlands in the northern Florida panhandle. Blackwater streams contain a high dissolved organic C concentration which imparts a dark color to the water and contain low concentrations of nutrients. Redwater streams contain high concentrations of suspended clays and inorganic nutrients, such as N and P, compared to blackwater streams. Active bacterial and fungal biomass was determined by direct microscopy; cellulose and lignin degradation were measured radiometrically. The experimental design was a randomized block. Treatments were: soil type (blackwater or redwater forested wetlands) and N additions (soils amended with the equivalent of 0, 200 or 400 kg N ha-1 as NH4NO3 ). Redwater soils contained higher concentrations of C, total N, P, K, Ca, Mn, Fe, B and Zn than blackwater soils. After N addition and 15 weeks of incubation, the active bacterial biomass in redwater soils was lower than in blackwater soils; the active bacterial biomass in blackwater soils was lower when 400 kg N ha', but not when 200 kg N ha-1 , was added. The active fungal biomass in blackwater soils was higher when 400 kg N ha-1 , but not when 200 kg N ha-1 , was added. The active fungal biomass in redwater wetland soils was lower when 200 kg N ha-1 , but not when 400 kg N ha-1 , was added. Cellulose and lignin degradation was higher in redwater than in blackwater soils. After 10 and 15 weeks of incubation, the addition of 200 or 400 kg N as NH4NO3 ha-1 decreased cellulose and lignin degradation in both wetland soils to similar levels. This study indicated that the addition of N may slow organic matter degradation and nutrient mineralization, thereby creating deficiencies of other plant-essential nutrients in wetland forest soils.

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“…Three slides were evaluated from each sample and ten fields per slide were evaluated with phase contrast microscopy for total hyphal length, and three transects were evaluated for FDA-stained (active) hyphal length at x 160 total magnification. lodonitrotetrazolium (INT) stain was used for counting active bacteria (Stamatiadis et al 1990). A 1-ml sample of initial soil suspension was diluted to a final dilution in a 0.2-mg soil in 4 ml buffer.…”

Section: Microbial Biomass Measurementsmentioning

confidence: 99%

Influence of nitrogen on atrazine and 2, 4 dichlorophenoxyacetic acid mineralization in blackwater and redwater forested wetland soils

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1999

Biology and Fertility of Soils

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Microcosms were used to determine the influence of N additions on active bacterial and fungal biomass, atrazine and dichlorophenoxyacetic acid (2,4-D) mineralization at 5, 10 and 15 weeks in soils from blackwater and redwater wetland forest ecosystems in the northern Florida Panhandle. Active bacterial and fungal biomass was determined by staining techniques combined with direct microscopy. Atrazine and 2,4-D mineralization were measured radiometrically. Treatments were: soil type, (blackwater or redwater forested wetland soils) and N additions (soils amended with the equivalent of 0, 200 or 400 kg N ha" as NH 4NO3). Redwater soils contained higher concentrations of C, total N, P, K, Ca, Mn, Fe, B and Zn than blackwater soils. After N addition and 15 weeks of incubation, active bacterial biomass in redwater soils was lower when N was added. Active bacterial biomass in blackwater soils was lower when 400 kg N ha-1 , but not when 200 kg N ha', was added. Active fungal biomass in blackwater soils was higher when 400 kg N ha-1 , but not when 200 kg N ha', was added. Active fungal biomass in redwater soils was lower when 200 kg N ha-1 , but not when 400 kg N ha', was added. After 15 weeks of incubation 2,4-D degradation was higher in redwater wetland soils than in blackwater soils. After 10 and 15 weeks of incubation the addition of 200 or 400 kg N ha' decreased both atrazine and 2,4-D degradation in redwater soils. The addition of 400 kg N ha' decreased 2,4-D degradation but not atrazine degradation in blackwater soils after 10 and 15 weeks of incubation. High concentrations of N in surface runoff and groundwater resulting from agricultural operations may have resulted in the accumulation of N in many wetland soils. Large amounts of N accumulating in wetlands may decrease mineralization of toxic agricultural pesticides.

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Use of staining and inhibitors to separate fungal and bacterial activity in soil

Cited by 73 publications

References 19 publications

Influence of irrigated agriculture on soil microbial diversity

Influence of irrigated agriculture on soil microbial diversity

Influence of nitrogen on cellulose and lignin mineralization in blackwater and redwater forested wetland soils

Influence of nitrogen on atrazine and 2, 4 dichlorophenoxyacetic acid mineralization in blackwater and redwater forested wetland soils

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