Use of stable isotopes to evaluate the functional effects of nutrients

Beylot, M.

doi:10.1097/01.mco.0000247480.17829.34

Cited by 13 publications

(6 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To increase reliability of the results, at least on cholesterol absorption, we followed the recommendation to use two markers, sitosterol and campesterol, instead of one (62). Our findings require further confirmation through the stable isotope technique (63).…”

Section: Discussionmentioning

confidence: 99%

Plasma cholesterol homeostasis, HDL remodeling and function during the acute phase reaction

Zimetti

Vuono

Gomaraschi

et al. 2017

Journal of Lipid Research

View full text Add to dashboard Cite

Acute phase reaction (APR) is a systemic inflammation triggered by several conditions associated with lipid profile alterations. We evaluated whether APR also associates with changes in cholesterol synthesis and absorption, HDL structure, composition, and cholesterol efflux capacity (CEC). We analyzed 59 subjects with APR related to infections, oncologic causes, or autoimmune diseases and 39 controls. We detected no difference in markers of cholesterol synthesis and absorption. Conversely, a significant reduction of LpA-I- and LpAI:AII-containing HDL (-28% and -44.8%, respectively) and of medium-sized HDL (-10.5%) occurred in APR. Total HDL CEC was impaired in APR subjects (-18%). Evaluating specific CEC pathways, we found significant reductions in CEC by aqueous diffusion and by the transporters scavenger receptor B-I and ABCG1 (-25.5, -41.1 and -30.4%, respectively). ABCA1-mediated CEC was not affected. Analyses adjusted for age and gender provided similar results. In addition, correcting for HDL-cholesterol (HDL-C) levels, the differences in aqueous diffusion total and ABCG1-CEC remained significant. APR subjects displayed higher levels of HDL serum amyloid A (+20-folds; = 0.003). In conclusion, APR does not associate with cholesterol synthesis and absorption changes but with alterations of HDL composition and a marked impairment of HDL CEC, partly independent of HDL-C serum level reduction.

show abstract

Section: Discussionmentioning

confidence: 99%

Plasma cholesterol homeostasis, HDL remodeling and function during the acute phase reaction

Zimetti

Vuono

Gomaraschi

et al. 2017

Journal of Lipid Research

View full text Add to dashboard Cite

show abstract

“…First, double-tracer methods 25 , which determine orally absorbed tracer molecules and their plasma concentration must be compensated for metabolic losses with the aid of second, intravenously applied tracer. Hence, these methods are very expensive.…”

Section: Methods For Determination Of Cholesterol Synthesis and Absormentioning

confidence: 99%

Hypocholesterolemia in Clinically Serious Conditions - Review

Vyroubal¹,

Chiarla²,

Giovannini³

et al. 2008

BIOMED PAP

View full text Add to dashboard Cite

Background:Cholesterol is an essential component of cell membranes, precursor of steroids, biliary acids and other components of serious importance in live organism. Cholesterol synthesis is a complicated and energy-demanding process. Real daily need of cholesterol and mechanisms of decline cholesterol levels in critical ill are unknown. During stressful situations a signifi cant hypocholesterolaemia may be found. Hypocholesterolemia has been known for a number of years to be a signifi cant prognostic indicator of increased morbidity and mortality connected with a whole spectrum of pathological conditions. The aim of article is the elucidation of the role and importance of hypocholesterolaemia during the intensive care. . Methods and Results:We examined studies that are engaged in problems of hypocholesterolemia in critically ill. Very low levels of total as well as LDL cholesterol are most frequently found in serious polytrauma, after extensive surgery, in serious infections, in protracted hypovolemic shock. It is still not clear whether hypocholesterolemia refl ects only a serious metabolic disorder, which results from a life-threatening condition, or whether it has an active role in evolution and outcome.Conclusions: Hypocholesterolemia is commonly observed in critically ill patients. Nevertheless, it is not known whether it is a secondary manifestation of disease, or whether it actively contributes to deterioration of the disease. Although the contribution of hypocholesterolemia to mortality is modest compared with known risk factors such as increased severity of illness and the development of nosocomial infection, low serum lipid concentrations represent a potential therapeutic target in sepsis.

show abstract

“…This test has a high correlation with maximal lipase production after hormonal stimulation, indicating that this is an excellent test to determine lipase activity in the duodenum. The 13 C‐labelled substrate for pancreatic enzymes is administered orally in a test breakfast; after digestion, 13 CO 2 is released, which can be measured in breath air (Beylot, ; Domínguez‐Muñoz et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…The following tests were performed: 13 C‐labelled triglycerides digestion test: This test is a breath test that allows for the evaluation of digestive function (Beylot, ; Domínguez‐Muñoz, Iglesias‐García, Vilariño‐Insua, & Iglesias‐Rey, ). After fasting overnight, on the morning of the study, 10 mg metoclopramide was administered to ensure gastric emptying.…”

Section: Methodsmentioning

confidence: 99%