Use of Soybean Meal Supplemented with Cell Bound Phytase for Replacement of Fish Meal in the Diet of Juvenile Milkfish, Chanos chanos

Hassan, Sajad; Altaff, K.; Satyanarayana, T.

doi:10.3923/pjn.2009.341.344

Cited by 21 publications

(12 citation statements)

References 4 publications

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“…fumigatus phytase (Mayer et al, 1999). Based on the biochemical characteristics, the phytase of P. anomala has been shown to be a suitable supplement for dephytinization of animal feeds and soymilk Hassan et al, 2009;Kaur and Satyanarayana, 2010). A direct comparison of recombinant Pphyp yield with that of P. anomala in cane molasses medium (Vohra and Satyanarayana, 2004) has not been made.…”

Section: Discussionmentioning

confidence: 98%

“…The cell-bound phytase from P. anomala has been shown to be a suitable supplement for dephytinization of animal feeds Hassan et al, 2009) and soymilk (Kaur and Satyanarayana, 2010). Therefore, to explore ways of optimizing yields of the enzyme, the corresponding gene was cloned and overexpressed in various yeast species in order to select the best host for the production of recombinant phytase.…”

Section: Introductionmentioning

confidence: 99%

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Pphy—A cell-bound phytase from the yeast Pichia anomala: Molecular cloning of the gene PPHY and characterization of the recombinant enzyme

Kaur

Singh

Böer

et al. 2010

Journal of Biotechnology

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Pphy—A cell-bound phytase from the yeast Pichia anomala: Molecular cloning of the gene PPHY and characterization of the recombinant enzyme

Kaur

Singh

Böer

et al. 2010

Journal of Biotechnology

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“…The cell-bound phytase of P. anomala has been found useful for supplementing feed of marine as well as fresh water fishes. The excretion of phosphorus was higher in the feeding trials of marine milk fish, Chanos chanos with soybean based feed compared to the same feed supplemented with 1,000 U kg -1 of cell bound phytase indicating that soybean meal supplemented with phytase can be used for the mass culture of milkfish (Hassan et al 2009). The fresh water fishes Rohu (Labeo rohita) (Fig.…”

mentioning

confidence: 96%

Production, characteristics and applications of the cell-bound phytase of Pichia anomala

Vohra

Kaur

Satyanarayana

2010

Antonie van Leeuwenhoek

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Among several yeasts isolated from dried flowers of Woodfordia fruticosa, Pichia anomala produced a high titre of cell-bound phytase. The optimization of fermentation variables led to formulation of media and selection of cultural variables that supported enhanced phytase production. The enzyme productivity was very high in fed batch fermentation in air-lift fermentor as compared to that in stirred tank fermentor. Amelioration in the cell-bound phytase activity was observed when yeast cells were permeabilized with Triton-X-100. The enzyme is thermostable and acid stable with broad substrate specificity, the characteristics that are desirable for enzymes to be used in the animal feed industry. The phytase-encoding gene was cloned and sequenced. The 3D structure of the enzyme was proposed by comparative modeling using phytase of Debaryomyces occidentalis (50% sequence identity) as template. When broiler chicks, and fresh water and marine fishes were fed with the feed supplemented with yeast biomass containing phytase, improvement in growth and phosphorus retention, and decrease in the excretion of phosphorus in the faeces were recorded. The cell-bound phytase of P. anomala could effectively dephytinize wheat flour and soymilk.

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“…The phytase of P. anomala exhibits several desirable characteristics (Vohra and Satyanarayana 2002a), and thus has a potential application as a feed supplement in poultry (Satyanarayana and Vohra 2003; Vohra et al. 2006) and aquaculture (Hassan et al. 2009).…”

Section: Discussionmentioning

confidence: 99%

“…In poultry (Vohra et al. 2006) and marine aquaculture (Hassan et al. 2009), its beneficial effect has already been demonstrated.…”

Section: Introductionmentioning

confidence: 99%

Improvement in cell-bound phytase activity ofPichia anomalaby permeabilization and applicability of permeabilized cells in soymilk dephytinization

Kaur

Satyanarayana

2009

Journal of Applied Microbiology

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Aims: Whole cell permeabilization of Pichia anomala to ameliorate the cell‐bound phytase activity and usability of permeabilized cells in dephytinization of soymilk. Methods and Results: The cells of P. anomala were subjected to permeabilization using the surfactant Triton X‐100 to overcome the permeability barrier and prepare whole cell biocatalysts with high phytase activity. The statistical approach, response surface methodology (RSM) was used to optimize the operating conditions for permeabilization. The treatment of cells with 5% Triton X‐100 for 30 min resulted in c. 15% enhancement in cell‐bound phytase activity. The shrinkage of protoplast was observed, although cell viability and phytase stability were not significantly altered. The free as well as immobilized permeabilized cells hydrolysed soymilk phytate, and the latter could be reused over four consecutive cycles. Conclusions: Whole cell permeabilization of P. anomala using Triton X‐100 led to enhancement in cell‐bound phytase activity. The viability and integrity of yeast cells were not significantly affected because of permeabilization. The permeabilized P. anomala cells effectively dephytinized soymilk, and the permeabilized cells immobilized in alginate could be reused because of sustained phytase activity. Significance and Impact of the Study: This is the first report on the use of permeabilized yeast cells for mitigating phytate content of soymilk. Alginate entrapment of permeabilized P. anomala allows reuse of cells for soymilk dephytinization, thus suggesting a potential application in food industry.

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Use of Soybean Meal Supplemented with Cell Bound Phytase for Replacement of Fish Meal in the Diet of Juvenile Milkfish, Chanos chanos

Cited by 21 publications

References 4 publications

Pphy—A cell-bound phytase from the yeast Pichia anomala: Molecular cloning of the gene PPHY and characterization of the recombinant enzyme

Pphy—A cell-bound phytase from the yeast Pichia anomala: Molecular cloning of the gene PPHY and characterization of the recombinant enzyme

Production, characteristics and applications of the cell-bound phytase of Pichia anomala

Improvement in cell-bound phytase activity ofPichia anomalaby permeabilization and applicability of permeabilized cells in soymilk dephytinization

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