Use of sol-gel techniques in the development of surface-enhanced Raman scattering (SERS) substrates suitable for in situ detection of chemicals in sea-water

Murphy, Thomas M.; Schmidt, Heinar; Kronfeldt, H.-D.

doi:10.1007/s003400050787

Cited by 49 publications

(43 citation statements)

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“…This should have great applications in rapid quantification of BaP in complex media such as environmental waters, as BaP is considered as a PAHs tracer. Furthermore, SERS detection of PAHs would be most suitable for in situ applications than standard method as GC/MS as shown by the pioneering works (Murphy et al 1999;Schmidt et al 2004;Péron et al 2009aPéron et al , b, 2010Pfannkuche et al 2012). Moreover, previous works carried out by Pfannkuche et al show the good agreement between SERS in situ measurements and laboratory GC/MS analyses.…”

Section: Discussionmentioning

confidence: 79%

“…Concomitantly, the low solubility of BaP in the aqueous media requires a method which is not only sensitive but also selective sensors takes into consideration the variety of the encountered molecules in the media. Hydrophobic SERS-active substrates could offer the expected solution for PAHs as shown in pioneer works (Bao et al 2012;Murphy et al 1999;Péron et al 2009aPéron et al , b, 2010Pfannkuche et al 2012;Schmidt et al 2004;Lin et al 2014;Gu et al 2013). But, in order to ensure selectivity and sensitivity to the sensor towards BaP, immunoassay is a powerful technique based on the specific interaction between the antigen and the complementary antibody.…”

Section: Introductionmentioning

confidence: 97%

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Organometallic nanoprobe to enhance optical response on the polycyclic aromatic hydrocarbon benzo[a]pyrene immunoassay using SERS technology

Dribek

Rinnert

Colas

et al. 2014

Environ Sci Pollut Res

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We demonstrated the use of a new organometallic nanoprobe for competitive surface-enhanced Raman scattering (SERS) immunoassay devoted to the detection of polycyclic aromatic hydrocarbons (PAH) such as benzo[a]pyrene (BaP) in seawater. The nanoprobes are gold nanoparticles (GNPs) labeled by a Raman reporter, the 5,5'-dithiobis(succinimidyl-2-nitrobenzoate) (DSNB) and functionalized with monoclonal antibodies anti-BaP. The antibodies are bound with a high specificity to the analyte while the GNPs enhanced the Raman scattering of the DSNB. This type of immunoassay involved the grafting of BaP onto a sensing surface. Thus, NH-terminated self-assembled monolayer is formed on the surface of gold substrate using cysteamine. Amines finally reacted with 6-formylbenzo[a]pyrene. So, this SERS detection involves four steps: (i) the nanoprobes are incubated with the sample; (ii) a drop of the mixture is then put onto the substrate; (iii) the surface is rinsed; and (iv) the surface is analyzed by Raman spectroscopy. To synthesize the nanoprobes, firstly, we prepared GNPs according to Frens' method. Then, GNPs were spontaneously labeled by the DSNB Raman reporter, thanks to a strong gold-sulfur interaction. Thereafter, BaP antibodies were cross-linked to the DSNB labeled GNPs by reaction of proteins primary amino groups with N-hydroxyl succinimide (NHS). Before use in SERS detection, their activity was controlled by surface plasmon resonance technique. The present method allows us to detect BaP at trace concentration (2 nmol/L). The results demonstrate that the proposed method has a great potential for application in the monitoring of seawater.

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Section: Discussionmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 97%

Organometallic nanoprobe to enhance optical response on the polycyclic aromatic hydrocarbon benzo[a]pyrene immunoassay using SERS technology

Dribek

Rinnert

Colas

et al. 2014

Environ Sci Pollut Res

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show abstract

“…Indeed, pursuit of raw enhancing power without a degree of selectivity is likely to lead to significant problems when fully validated analytical procedures based on these materials need to be developed. While there have been some demonstrations of successful analyses carried out using unmodified media in complex matrices such as saliva [36], sea water [37] and fermentation broths [38], these particular methods work because potentially interfering compounds fortuitously give low SERS signals. This can be either because they are in low concentration and do not adsorb to the surface or they give bands which lie in a spectral region that does not interfere with the analyte signal (e.g.…”

Section: Selectivitymentioning

confidence: 99%

Quantitative SERS Methods

Bell¹,

Stewart²

2010

Surface Enhanced Raman Spectroscopy

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The goal of transforming SERS from an interesting novelty into a viable quantitative analytical technique has been pursued for many years, but it is only recently that the first indications that quantitative SERS could be generally achievable have appeared. Up to this point, the challenges of preparing sensitive and reproducible enhancing media and the need to develop a deep understanding of the fundamental mechanisms of the effect (or at least to have a working knowledge of the relationship between the microstructure of the enhancing materials, their surface chemistry and the enhancements that they provide; see also Chapter 1) have been sufficient to occupy the attention of researchers who were interested in using SERS for quantitative measurements. However, many of these issues have now either been fully resolved or at least brought to the stage where they are no longer impediments. For example, new generations of enhancing media have been developed; the links between microstructure, plasmon resonances and enhancements have been established; and the role of the media's surface chemistry is being addressed. This chapter discusses these new developments and also considers the extent to which the separate strands can be combined to create standard, generally applicable methods for quantitative SERS. In addition, it highlights the need to begin the process of judging the success of new SERS methods against competing technologies, rather than against previous SERS results. SERS MediaThe choice of enhancing media for SERS studies has grown to an extraordinary extent, although they can still be broadly divided into solution-phase suspensions of nanoparticles and micro-textured solid substrates. This division dates back to the earliest work in the area, which used either colloidal suspensions of silver or gold nanoparticles [1] or the surfaces of roughened noble-metal electrodes [2]. Since this

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“…For the trace analysis of PAHs in an aqueous solution, several types of SERS sensor have been applied including silver colloid-based sol-gel films [7,8], tailor-made metal nanoparticles [9], self-assembled gold colloid films [10], partition layer-modified silver films over nanospheres [11] and dithiocarbamate calixarene (DTCX) functionalized silver colloids [12]. The sensitivity of a SERS substrate depends on the long-term electromagnetic enhancement in the vicinity of metal nanoparticles and the short-term chemical enhancement due to the charge transfer between the analyte and metal nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Naturally grown Ag nanoparticles on quartz substrates as SERS substrate excited by a 488 nm diode laser system for SERDS

et al. 2012

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A silver nanoparticle ensemble was prepared under ultrahigh vacuum (UHV) conditions by Volmer-Weber growth on a quartz substrate for surface-enhanced Raman scattering (SERS) investigations of pyrene molecules. To tune the surface plasmon resonance frequency in the vicinity of the excitation wavelength of 488 nm of the diode laser, the morphology of the silver nanoparticles was optimized. The substrates were mounted in a flow-through cell as part of the optical Raman set-up. A microsystem diode laser generates two slightly different emission wavelengths (λ = 487.61 nm and λ = 487.91 nm) with a spectral width <10 pm and an optical power of 20 mW, i.e. SERS experiments are possible but also shifted excitation Raman difference spectroscopy (SERDS) can be carried out. For trace analysis of pyrene in water we demonstrate SERS/SERDS experiments which lead to a limit of detection of 2 nmol/l for pyrene. These results suggest that with silver nanoparticle ensembles excited at their plasmon resonance at 488 nm combined SERS/SERDS measurements can be effectively performed for in-situ trace analysis of pollutant chemicals in water.

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Use of sol-gel techniques in the development of surface-enhanced Raman scattering (SERS) substrates suitable for in situ detection of chemicals in sea-water

Cited by 49 publications

References 1 publication

Organometallic nanoprobe to enhance optical response on the polycyclic aromatic hydrocarbon benzo[a]pyrene immunoassay using SERS technology

Organometallic nanoprobe to enhance optical response on the polycyclic aromatic hydrocarbon benzo[a]pyrene immunoassay using SERS technology

Quantitative SERS Methods

Naturally grown Ag nanoparticles on quartz substrates as SERS substrate excited by a 488 nm diode laser system for SERDS

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