Use of single cell gel electrophoresis assays for the detection of DNA-protective effects of dietary factors in humans: Recent results and trends

Hoelzl, Christine; Knasmüller, Siegfried; Mišík, Miroslav; Collins, Andrew; Dušinská, Maria; Nersesyan, Armen

doi:10.1016/j.mrrev.2008.07.004

Cited by 59 publications

(46 citation statements)

References 107 publications

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“…); free radical damage to DNA can result in mutations and thus eventually in cancer; antioxidants scavenge free radicals and prevent this damage; therefore, antioxidants must be responsible for the protection against cancer. There is a great deal of evidence that antioxidants, or antioxidant-rich foods, given to volunteers in intervention trials, do indeed decrease the level of endogenous DNA oxidation measured in lymphocytes and enhance the resistance of lymphocytes to oxidative damage ex vivo [2,3]. But the level of DNA base oxidation is now recognised to be far lower (by orders of magnitude) than was often supposed [4], and in any case, reactive oxygen species are involved in immune protection and in cell signalling pathways [5], so suppressing it too much might be detrimental.…”

Section: Introductionmentioning

confidence: 99%

Effects of micronutrients on DNA repair

2012

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Section: Introductionmentioning

confidence: 99%

Effects of micronutrients on DNA repair

2012

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“…to investigate the impact of dietary factors on DNA stability (Hoelzl et al, 2009). A limitation is that the comet assay detects only DNA fragmentation and does not clarify the exact mechanisms responsible for the formation of DNA damage.…”

Section: Acta Veterinaria Hungarica 61 2013mentioning

confidence: 99%

Dose-related genotoxic effect of T-2 toxin measured by comet assay using peripheral blood mononuclear cells of healthy pigs

Horvatovich

Hafner

Bodnár

et al. 2013

Acta Veterinaria Hungarica

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T-2 toxin is the most acutely toxic trichothecene mycotoxin: it inhibits protein, DNA and RNA synthesis. The main goal of this study was to evaluate the rate of DNA damage caused by T-2 toxin in porcine mononuclear cells in increasing concentrations (0.1, 0.5 and 1.0 µmol) and after two different incubation periods (24 and 42 h). The lowest concentration caused DNA damage and about 50% of the treated cells could be categorised as having 1 to 4 scores in comet assay. In parallel with the increase of T-2 toxin concentration, the frequency of intact lymphocytes decreased from 50.2% (0.1 μM) to 36.3% (1.0 μM) in the first 24 h. In case of score 3, the highest concentration of T-2 toxin resulted in a 5-fold change, as compared to the lowest dose. Cells with score 4 were found only after exposure to 1.0 μM T-2 toxin. The exposure time did not have a significant effect on the results, while concentration did (P < 0.0001). However, a significant interaction between concentration and time as fixed factors (P < 0.0001) was found. When these were combined as a single factor, the results showed a significant toxin treatment effect on the results. It was concluded that a time-and dose-dependent DNA damaging effect of T-2 toxin could be demonstrated using peripheral blood mononuclear cells from healthy pigs by comet assay.

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“…Several DNA repair enzymes recognize damaged bases, introducing breaks at sites of the base damage. Thus, inclusion of an extra step of nucleoid DNA digestion with lesion-specific enzymes following lysis, allow detection of modified bases increasing the sensitivity and specificity of the comet assay (Collins, 2009;Hoelzl et al, 2009). Endonuclease III (EndoIII) was the first enzyme used to recognize oxidized pyrimidines in DNA and to remove them, leaving an AP site that is subsequently converted in breaks at pH13.…”

Section: The Use Of Lesion-specific Enzymesmentioning

confidence: 99%

“…For more details see the following revisions, (Hoelzl et al, 2009;Moller and Loft, 2002;Wasson et al, 2008 andWong et al, 2005).…”

Section: Application Of the Comet Assay In Chemoprevention Studiesmentioning

confidence: 99%

DNA Damage Protection and Induction of Repair by Dietary Phytochemicals and Cancer Prevention: What Do We Know?

Ramos¹,

Lima²,

Pereira‐Wilson³

2011

Selected Topics in DNA Repair

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Use of single cell gel electrophoresis assays for the detection of DNA-protective effects of dietary factors in humans: Recent results and trends

Cited by 59 publications

References 107 publications

Effects of micronutrients on DNA repair

Effects of micronutrients on DNA repair

Dose-related genotoxic effect of T-2 toxin measured by comet assay using peripheral blood mononuclear cells of healthy pigs

DNA Damage Protection and Induction of Repair by Dietary Phytochemicals and Cancer Prevention: What Do We Know?

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