Use of Shake Cultures in a Semisolid Thioglycolate Medium for Differentiating Staphylococci from Micrococci1

Evans, James B.; Kloos, Wesley E.

doi:10.1128/aem.23.2.326-331.1972

Cited by 69 publications

(36 citation statements)

References 6 publications

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“…Negative, weak or delayed reactions were retested in Hugh and Leifson's tubed Medium. For tests on anaerobic growth Brewer's Thioglycolate Medium (Oxoid) plus 0.3 % (w/v) Bacto Agar (Difco), was inoculated as described by Evans & Kloos (1972).…”

Section: Methodsmentioning

confidence: 99%

Coagulase and Heat‐resistant Nuclease Producing Staphylococcus epidermidis Strains from Animals

Devriese

Oeding

1975

Journal of Applied Bacteriology

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Strains isolated from the skin and the upper respiratory tract of chickens and from bovine udders showed characteristics typical for Staphylococcus epidermidis biotype 2, but produced a heat‐resistant nuclease (DNase) and reacted in the tube coagulase test. The strains were physiologically, biochemically and serologically indistinguishable from Staphylococcus hyicus strains isolated from swine. However, in contrast to S. hyicus, they appeared to be devoid of pathogenicity when tested in colostrum deprived piglets. The poultry strains occur frequently as skin inhabitants.

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Section: Methodsmentioning

confidence: 99%

Coagulase and Heat‐resistant Nuclease Producing Staphylococcus epidermidis Strains from Animals

Devriese

Oeding

1975

Journal of Applied Bacteriology

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“…Staphyloccocus saprophyticus was separated from Micrococcus (Baird-Parker, 1974). Methods described by Evans & Kloos (1972) were used to distinguish Staphylococcus from Micrococcus genus.…”

Section: Percent Incidence Of Microbial Floramentioning

confidence: 99%

Quantitative microbiology of human vulva

1979

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The microbial flora of the vulva is described and compared with the flora of the forearm by utilizing the detergent scrub method. Microbial counts were higher on the vulva (2.8 x 10(6)/cm2) than on the forearm (6.4 x 10(2)/cm2). Lipophilic diphtheroids, coagulase negative staphylococci, micrococci, non-lipophilic diphtheroids and lactobacilli formed the dominant flora of the vulva. Streptococci, Gram negative rods and yeasts were also noted. The highest incidence of S. aureus was noted on the vulva (67%) followed by perianal (30%), nose (30%), and the forearm (11%).

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“…To determine the ability of bacterial isolates to grow under aerobic or anaerobic conditions, strains were streaked into test tubes of thioglycolate semi-solid medium with 0Á3% agar and then incubated at 37°C for 24 h(Evans and Kloos 1972).…”

mentioning

confidence: 99%

Isolation and characterization of Bacillus subtilis CH16 strain from chicken gastrointestinal tracts for use as a feed supplement to promote weight gain in broilers

Nguyen

Tran

et al. 2015

Lett Appl Microbiol

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Spore-forming bacterial strains were isolated from chicken gastrointestinal tracts to develop a heat-stable feed supplement that promotes weight gain in broilers. Seven Bacillus strains having more than 90% sporulation were screened from the isolates and identified to be closely related with Bacillus subtilis and Bacillus licheniformis. Of the seven strains, B. subtilis CH16 was selected to develop a feed supplement for broilers, because it formed 100% heat-stable spores, grew rapidly at 42°C and quickly formed a biofilm. In large-scale trials in broilers (n ≥ 1150 per group), the group fed CH16 (3 × 10(6) CFU g(-1) pellet) showed higher average daily gain (ADG = 61·16) and lower food conversion ratio (FCR = 1·696) than did the group fed B. licheniformis CH22 (ADG = 57·10 and FCR = 1·792), the group fed B. subtilis HU58 (ADG = 51·90 and FCR = 1·868), BioPlus group (ADG = 59·32 and FCR = 1·807) and the control group (ADG = 56·02 and FCR = 1·880). In conclusion, CH16 spores significantly increased ADG by 9·17% and reduced FCR by 9·79% in broilers. The result supports the use of B. subtilis CH16 of chicken intestinal origin as a feed supplement that promote weight gain in broilers. Significance and impact of the study: This study reports screening of Bacillus strains isolated from chicken gastrointestinal tracts for development of a feed supplement that promote weight gain in broilers. Of the seven Bacillus isolates with high sporulation efficiency (≥90%), Bacillus subtilis CH16 strain showed the best growth and biofilm formation at body temperature of broilers (42°C). In large-scale trials in broilers (n ≥ 1150 per group), CH16 spores induced a 9·17% increase in daily weight gain (ADG) and a 9·79% reduction in FCR while the commercial BioPlus(®) YC induced only a 5·89% increase in ADG and a 3·88% reduction in FCR.

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Use of Shake Cultures in a Semisolid Thioglycolate Medium for Differentiating Staphylococci from Micrococci1

Cited by 69 publications

References 6 publications

Coagulase and Heat‐resistant Nuclease Producing Staphylococcus epidermidis Strains from Animals

Coagulase and Heat‐resistant Nuclease Producing Staphylococcus epidermidis Strains from Animals

Quantitative microbiology of human vulva

Isolation and characterization of Bacillus subtilis CH16 strain from chicken gastrointestinal tracts for use as a feed supplement to promote weight gain in broilers

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