Use of Sera from Humans and Dolphins with Lacaziosis and Sera from Experimentally Infected Mice for Western Blot Analyses of
            <i>Lacazia loboi</i>
            Antigens

Mendoza, Leonel; Belone, Andréa de Faria Fernandes; Vilela, Raquel; Rehtanz, Manuela; Bossart, Gregory D.; Reif, John S.; Fair, Patricia A.; Durden, Wendy Noke; Leger, Judy St.; Travassos, Luiz R.; Rosa, Patrícia Sammarco

doi:10.1128/cvi.00201-07

Cited by 21 publications

(18 citation statements)

References 25 publications

(41 reference statements)

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“…Although the clinical and pathological features of lobomycosis in humans and dolphins are similar, potential molecular differences between the organism found in humans and dolphins may explain adaptation to the marine environment and lack of human cases among persons who use the IRL for occupational and recreational purposes. Recently, an antigenic relationship between Lacazia in dolphins and humans was shown by Western blot analyses of sera (Mendoza et al, 2008) although morphologic differences in the yeast cells found in the two species have been reported (Haubold et al, 2000).…”

Section: Discussionmentioning

confidence: 97%

Lobomycosis in Bottlenose Dolphins (Tursiops truncatus) from the Indian River Lagoon, Florida: Estimation of Prevalence, Temporal Trends, and Spatial Distribution

et al. 2008

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Lobomycosis (lacaziosis) is a chronic fungal disease of the skin that affects only dolphins and humans. Previous studies have shown a high prevalence of lobomycosis in bottlenose dolphins (Tursiops truncatus) from the Indian River Lagoon, Florida (IRL). We studied the occurrence and distribution of lobomycosis in the IRL using photo-identification survey data collected between 1996 and 2006. Our objectives were to (1) determine the sensitivity and specificity of photo-identification for diagnosis of lobomycosis in free-ranging dolphins; (2) determine the spatial distribution of lobomycosis in the IRL; and (3) assess temporal patterns of occurrence. Photographs from 704 distinctly marked dolphins were reviewed for skin lesions compatible with lobomycosis. The presumptive diagnosis was validated by comparing the results of photographic analysis with physical examination and histologic examination of lesion biopsies in 102 dolphins captured and released during a health assessment and 3 stranded dolphins. Twelve of 16 confirmed cases were identified previously by photography, a sensitivity of 75%. Among 89 dolphins without disease, all 89 were considered negative, a specificity of 100%. The prevalence of lobomycosis estimated from photographic data was 6.8% (48/704). Spatial distribution was determined by dividing the IRL into six segments based on hydrodynamics and geographic features. The prevalence ranged from <1% in the Mosquito Lagoon to 16.9% in the south Indian River. The incidence of the disease did not increase during the study period, indicating that the disease is endemic, rather than emerging. In summary, photo-identification is a useful tool to monitor the course of individual and population health for this enigmatic disease.

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Section: Discussionmentioning

confidence: 97%

Lobomycosis in Bottlenose Dolphins (Tursiops truncatus) from the Indian River Lagoon, Florida: Estimation of Prevalence, Temporal Trends, and Spatial Distribution

et al. 2008

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“…Studies using serum samples from humans and dolphins with lacaziosis/lobomycosis, mice experimentally infected with L. loboi , and serum samples from humans with paracocidioidomycosis showed that IgG in serum samples from dolphins and humans infected with L. loboi had strong cross-reactivity with the gp43 antigen of P. brasiliensis ( 4 , 6 , 21 ). These findings support the hypothesis that the uncultivated organism causing cutaneous granulomas in humans and dolphins was L. loboi .…”

Section: Discussionmentioning

confidence: 99%

“…Findings also implied that the gp43 antigen of the etiologic agent of parakeloidal-like granulomas in humans and dolphins was antigenically similar to that of P. brasiliensis . On the basis of these serologic studies ( 4 , 6 , 21 ), current phylogenetic data for gp43 and Kex gene exons, and ITS DNA sequences, placement of L. loboi from humans in its own genus is questionable. Efforts to culture the organism from dolphins on classical laboratory media successfully used to isolate P. brasiliensis from humans with paracoccidioidomycosis were not successful ( 4 , 7 ).…”

Section: Discussionmentioning

confidence: 99%

Cutaneous Granulomas in Dolphins Caused by Novel UncultivatedParacoccidioides brasiliensis

Vilela¹,

Bossart²,

Leger³

et al. 2016

Emerg. Infect. Dis.

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“…L. loboi cells found in infected tissues from T. truncatus are significantly smaller than those found in humans, which, according to Haubold et al (2000), suggests that the organism may not be identical in the 2 hosts. However, serological data indicate that dolphins and humans are infected with similar L. loboi strains (Mendoza et al 2008). In humans, lobomycosis is a chronic fungal infection of the skin, endemic in rural regions in South and Central America.…”

Section: Lobomycosismentioning

confidence: 99%

Emerging infectious diseases in cetaceans worldwide and the possible role of environmental stressors

Bressem¹,

Raga²,

Guardo³

et al. 2009

Dis. Aquat. Org.

266

270

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We reviewed prominent emerging infectious diseases of cetaceans, examined their potential to impact populations, re-assessed zoonotic risk and evaluated the role of environmental stressors. Cetacean morbilliviruses and papillomaviruses as well as Brucella spp. and Toxoplasma gondii are thought to interfere with population abundance by inducing high mortalities, lowering reproductive success or by synergistically increasing the virulence of other diseases. Severe cases of lobomycosis and lobomycosis-like disease (LLD) may contribute to the death of some dolphins. The zoonotic hazard of marine mammal brucellosis and toxoplasmosis may have been underestimated, attributable to frequent misdiagnoses and underreporting, particularly in developing countries and remote areas where carcass handling without protective gear and human consumption of fresh cetacean products are commonplace. Environmental factors seem to play a role in the emergence and pathogenicity of morbillivirus epidemics, lobomycosis/LLD, toxoplasmosis, poxvirus-associated tattoo skin disease and, in harbour porpoises, infectious diseases of multifactorial aetiology. Inshore and estuarine cetaceans incur higher risks than pelagic cetaceans due to habitats often severely altered by anthropogenic factors such as chemical and biological contamination, direct and indirect fisheries interactions, traumatic injuries from vessel collisions and climate change.

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Use of Sera from Humans and Dolphins with Lacaziosis and Sera from Experimentally Infected Mice for Western Blot Analyses of Lacazia loboi Antigens

Cited by 21 publications

References 25 publications

Lobomycosis in Bottlenose Dolphins (Tursiops truncatus) from the Indian River Lagoon, Florida: Estimation of Prevalence, Temporal Trends, and Spatial Distribution

Lobomycosis in Bottlenose Dolphins (Tursiops truncatus) from the Indian River Lagoon, Florida: Estimation of Prevalence, Temporal Trends, and Spatial Distribution

Cutaneous Granulomas in Dolphins Caused by Novel UncultivatedParacoccidioides brasiliensis

Emerging infectious diseases in cetaceans worldwide and the possible role of environmental stressors

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