Use of sensitivity-enhanced nuclear magnetic resonance spectroscopy equipped with a 1.7-mm cryogenically cooled micro-coil probe in identifying human sperm intracellular metabolites
Aswathi Cheredath,
Shubhashree Uppangala,
Ameya Jijo
et al.
Abstract:Context The clinical value of human sperm metabolites has not been established due to the technical complexity in detecting these metabolites when sperm numbers are low. Aims To detect endogenous intracellular metabolites in fresh and post-thaw human spermatozoa using 800 MHz nuclear magnetic resonance (NMR) spectroscopy equipped with a 1.7-mm cryo-probe. Methods Processed spermatozoa from 25 normozoospermic ejaculates were subjected to extraction of intracellular metabolites and then profiled by… Show more
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