Use of <scp>l</scp>-pNIPAM hydrogel as a 3D-scaffold for intestinal crypts and stem cell tissue engineering

Dosh, Rasha Hatem; Jordan-Mahy, Nicola; Sammon, Chris; Maitre, Christine L. Le

doi:10.1039/c9bm00541b

Cited by 24 publications

(16 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even though cell function was observed to decline after a few weeks of culture, these systems offer a model with many physiological similarities to the small intestine particularly useful for short-term studies regarding bacterial-epithelial interaction [186] and potentially intestinal permeability studies. Interestingly, a long-term 3D model of the small intestinal epithelium was recently established by Dosh et al, using Caco-2 and HT29-MTX cells co-cultured on a hydrogel (L-pNIPAM) scaffold [187]. This model was particularly developed to facilitate long-term mechanistic studies in an inflammatory environment, similar to that in IBD [188].…”

Section: Co-culture Of Cell Linesmentioning

confidence: 99%

The Intestinal Barrier and Current Techniques for the Assessment of Gut Permeability

Schoultz

Keita

2020

Cells

234

179

View full text Add to dashboard Cite

The intestinal barrier is essential in human health and constitutes the interface between the outside and the internal milieu of the body. A functional intestinal barrier allows absorption of nutrients and fluids but simultaneously prevents harmful substances like toxins and bacteria from crossing the intestinal epithelium and reaching the body. An altered intestinal permeability, a sign of a perturbed barrier function, has during the last decade been associated with several chronic conditions, including diseases originating in the gastrointestinal tract but also diseases such as Alzheimer and Parkinson disease. This has led to an intensified interest from researchers with diverse backgrounds to perform functional studies of the intestinal barrier in different conditions. Intestinal permeability is defined as the passage of a solute through a simple membrane and can be measured by recording the passage of permeability markers over the epithelium via the paracellular or the transcellular route. The methodological tools to investigate the gut barrier function are rapidly expanding and new methodological approaches are being developed. Here we outline and discuss, in vivo, in vitro and ex vivo techniques and how these methods can be utilized for thorough investigation of the intestinal barrier.

show abstract

Section: Co-culture Of Cell Linesmentioning

confidence: 99%

The Intestinal Barrier and Current Techniques for the Assessment of Gut Permeability

Schoultz

Keita

2020

Cells

234

179

View full text Add to dashboard Cite

show abstract

“…Animal models have usually been constructed and used to study intestinal diseases. For the construction of such models (Dosh et al, 2019), there are various methods for causing defects in the mucus layer of animal intestine, including chemical treatment, mucin-related gene defection, specific disruption of intestinal epithelial cells, and immune cell deformation (Table 2).…”

Section: Development Of Disease Models From In Vitro Modelsmentioning

confidence: 99%

“…In the intestinal model, glycan is added to the mucus layer via a mucus-secreting cell line [HT29 methotrexate (MTX) cells, LS174T] or goblet cells. For example, HT-29 MTX was cultured with Caco-2 in various ratios (1:9-3:7) or stem cells were differentiated into several intestinal epithelial cell types (enterocytes, goblet cells, and enteroendocrine cells) to mimic the intestinal and colonic mucosa (Dosh et al, 2019). To create disease model from an in vitro model, various methods are used.…”

Section: Development Of Disease Models From In Vitro Modelsmentioning

confidence: 99%

In vitro Models of the Small Intestine for Studying Intestinal Diseases

Jung

Kim

2022

Front. Microbiol.

View full text Add to dashboard Cite

The small intestine is a digestive organ that has a complex and dynamic ecosystem, which is vulnerable to the risk of pathogen infections and disorders or imbalances. Many studies have focused attention on intestinal mechanisms, such as host–microbiome interactions and pathways, which are associated with its healthy and diseased conditions. This review highlights the intestine models currently used for simulating such normal and diseased states. We introduce the typical models used to simulate the intestine along with its cell composition, structure, cellular functions, and external environment and review the current state of the art for in vitro cell-based models of the small intestine system to replace animal models, including ex vivo, 2D culture, organoid, lab-on-a-chip, and 3D culture models. These models are described in terms of their structure, composition, and co-culture availability with microbiomes. Furthermore, we discuss the potential application for the aforementioned techniques to these in vitro models. The review concludes with a summary of intestine models from the viewpoint of current techniques as well as their main features, highlighting potential future developments and applications.

show abstract

“…Hydrogels are used in products such as contact lenses and form the basis of cellular structure. [3][4][5] Furthermore, hydrogels have been assessed for their various biomedical applications [6][7][8] including bio-alternatives. However, hydrogels are susceptible to bacterial contamination owing to their high water content and application in humid environments.…”

Section: Introductionmentioning

confidence: 99%

Bactericidal effect of cationic hydrogels prepared from hydrophilic polymers

Shibata

Kurokawa

Aizawa

et al. 2020

J of Applied Polymer Sci

View full text Add to dashboard Cite

This study investigated whether hydrogels comprising hydrophilic cationic polymers have similar bactericidal effects. Bacteria were seeded on hydrogels and agar and their viability was assessed with time. Cationic hydrogels displayed bactericidal effects upon long‐term bacterial contact. Furthermore, we assessed the areal density of cationic monomer unit of the cationic hydrogels, water content, and the initial elastic modulus. We examined correlations between each factor and bacterial death ratios; consequently, the bacterial death ratios were strongly correlated with the areal density of cationic hydrogel monomers. Elastic energy (Wel) generated at the cytomembrane ion‐binding region and the cationic hydrogel and the cytomembrane interfacial energy (Wf) were estimated; consequently, Wel exceeded Wf at higher contact areas. The cationic hydrogel may extract cytomembranes with a reasonable adsorption area. Therefore, cationic hydrogels may be used as probes for ultrasonic echo to sterilize medical equipment.

show abstract

Use of l-pNIPAM hydrogel as a 3D-scaffold for intestinal crypts and stem cell tissue engineering

Abstract: Intestinal stem cells hold great potential in tissue regeneration of the intestine, however, there are key limitations in their culture in vitro.

Cited by 24 publications

References 57 publications

The Intestinal Barrier and Current Techniques for the Assessment of Gut Permeability

The Intestinal Barrier and Current Techniques for the Assessment of Gut Permeability

In vitro Models of the Small Intestine for Studying Intestinal Diseases

Bactericidal effect of cationic hydrogels prepared from hydrophilic polymers

Contact Info

Product

Resources

About