Use of Schistosoma mansoni soluble egg antigen (SEA) for antibody detection and diagnosis of schistosomiasis: The need for improved accuracy evaluations of diagnostic tools

Graeff-Teixeira, Carlos; Favero, Vivian; Souza, Renata Perotto de; Pascoal, Vanessa Fey; Bittencourt, Hélio Radke; Fukushige, Mizuho; Geiger, Stefan Michael; Negrão-Corrêa, Déborah

doi:10.1016/j.actatropica.2020.105800

Cited by 12 publications

(6 citation statements)

References 59 publications

(112 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The antibody based tests described in this review have several advantages compared to the parasitological diagnosis. These include high sensitivity over parasitological methods, relatively easy to automation, large scale processing of samples and POC-ICT are less time consuming [ 6 , 36 , 65 ]. However, the antibody based tests also have several limitations.…”

Section: Resultsmentioning

confidence: 99%

“…Schistosome-specific antibodies remain detectable for a long period after the infection has been cured. Consequently, measuring anti-schistosome antibody titres in serum may not discriminate between current and past infections [ 36 , 65 ]. Cross reactivity between antigens from Schistosoma species and other co-endemic helminths such as soil transmitted helminths may occur due to shared antigenic epitopes [ 36 , 65 ].…”

Section: Resultsmentioning

confidence: 99%

“…Consequently, measuring anti-schistosome antibody titres in serum may not discriminate between current and past infections [ 36 , 65 ]. Cross reactivity between antigens from Schistosoma species and other co-endemic helminths such as soil transmitted helminths may occur due to shared antigenic epitopes [ 36 , 65 ]. Antibody concentrations do not necessarily correlate with infection intensity [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

“…This has clinical implications in confirmation of successful praziquantel treatment since specific antibodies may persist long after the worms have disappeared [ 36 ]. Lack of reproducibility between different batches of reagents may further hinder or delay implementation of new diagnostics [ 65 ].…”

Section: Resultsmentioning

confidence: 99%

See 3 more Smart Citations

Diagnostic performances of Schistosoma haematobium and Schistosoma mansoni recombinant proteins, peptides and chimeric proteins antibody based tests. Systematic scoping review

Vengesai

Muleya²,

Midzi³

et al. 2023

PLoS ONE

View full text Add to dashboard Cite

Background Traditional diagnostic tests for schistosome infections are suboptimal, particularly when the parasite burden is low. In the present review we sought to identify recombinant proteins, peptides, and chimeric proteins with potential to be used as sensitive and specific diagnostic tools for schistosomiasis. Methods The review was guided by PRISMA-ScR guidelines, Arksey and O’Malley’s framework, and guidelines from the Joanna Briggs Institute. Five databases were searched: Cochrane library, PubMed, EMBASE, PsycInfo and CINAHL, alongside preprints. Identified literature were assessed by two reviewers for inclusion. A narrative summary was used to interpret the tabulated results. Results Diagnostic performances were reported as specificities, sensitivities, and AUC. The AUC for S. haematobium recombinant antigens ranged from 0.65 to 0.98, and 0.69 to 0.96 for urine IgG ELISA. S. mansoni recombinant antigens had sensitivities ranging from 65.3% to 100% and specificities ranging from 57.4% to 100%. Except for 4 peptides which had poor diagnostic performances, most peptides had sensitivities ranging from 67.71% to 96.15% and specificities ranging from 69.23% to 100%. S. mansoni chimeric protein was reported to have a sensitivity of 86.8% and a specificity of 94.2%. Conclusion The tetraspanin CD63 antigen had the best diagnostic performance for S. haematobium. The tetraspanin CD63 antigen Serum IgG POC-ICTs had a sensitivity of 89% and a specificity of 100%. Peptide Smp_150390.1 (216–230) serum based IgG ELISA had the best diagnostic performance for S. mansoni with a sensitivity of 96.15% and a specificity of 100%. Peptides were reported to demonstrate good to excellent diagnostic performances. S. mansoni multi-peptide chimeric protein further improved the diagnostic accuracy of synthetic peptides. Together with the advantages associated with urine sampling technique, we recommend development of multi-peptide chimeric proteins urine based point of care tools.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Diagnostic performances of Schistosoma haematobium and Schistosoma mansoni recombinant proteins, peptides and chimeric proteins antibody based tests. Systematic scoping review

Vengesai

Muleya²,

Midzi³

et al. 2023

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…This diagnostic approach may be suitable for acute and pre-patent infections which ordinarily pose a diagnostic challenge to SEA or SWAP. We recommend the consideration of the proposed components for descriptions when reporting development and/or evaluation of serological diagnostic methods 22 . Further studies to identify, purify, and recombinantly produce the immunoreactive protein are highly recommended.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of schistosomula crude antigen (SCA) as a diagnostic tool for Schistosoma mansoni in low endemic human population

Oyeyemi

Corsini

Gonçalves

et al. 2021

Sci Rep

View full text Add to dashboard Cite

The study aimed to determine the potential of schistosomula crude antigen (SCA) as a diagnostic target for anti-S. mansoni antibody detection. Cercariae were transformed into schistosomula, homogenized through sonication, and then centrifuged to obtain the SCA. SCA was evaluated using ELISA and dot blots immunoassays on 30 S. mansoni infected sera samples obtained from chronic patients and 30 non-infected humans’ sera samples. Either Kato-Katz or saline gradient method or both were employed as the diagnostic reference. Dot blots immunoassay was further performed on protein eluted from 10 to 12 kDa immunoreactive band identified by Western blot analysis. The area under the ROC curve was 0.95 (AUC 0.95, CI 0.88–1.01, p < 0.0001). The sensitivity and specificity of SCA-ELISA and dot blots assays were 96.67% and 86.67% respectively. The human IgG-specific response against SCA was significantly higher in S. mansoni infected individuals (OD = 0.678 ± 0.249) compared to the non-infected population (OD = 0.235 ± 0.136) (p < 0.0001). Our study showed that SCA and its 10–12 kDa component could be useful as diagnostic tools for chronic schistosomiasis.

show abstract

Schistosomiasis Control: Present Situation and Perspectives

Graeff-Teixeira,

Pieri

2022

Infectious Tropical Diseases and One Health in Latin America

View full text Add to dashboard Cite

Use of Schistosoma mansoni soluble egg antigen (SEA) for antibody detection and diagnosis of schistosomiasis: The need for improved accuracy evaluations of diagnostic tools

Cited by 12 publications

References 59 publications

Diagnostic performances of Schistosoma haematobium and Schistosoma mansoni recombinant proteins, peptides and chimeric proteins antibody based tests. Systematic scoping review

Diagnostic performances of Schistosoma haematobium and Schistosoma mansoni recombinant proteins, peptides and chimeric proteins antibody based tests. Systematic scoping review

Evaluation of schistosomula crude antigen (SCA) as a diagnostic tool for Schistosoma mansoni in low endemic human population

Schistosomiasis Control: Present Situation and Perspectives

Contact Info

Product

Resources

About