Use of RT–PCR on oral fluid samples to assist the identification of measles cases during an outbreak

Oliveira, Solange Artimos de; Siqueira, Marilda M.; Camacho, L. A. B.; Castro-Silva, Rosangela de; Bruno, Barbara; Cohen, B. J.

doi:10.1017/s0950268802007963

Cited by 15 publications

(9 citation statements)

References 19 publications

(29 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many other viruses are increasingly being detected directly by targeting DNA using PCR methodology. These include HPV [193], human herpes virus [194], herpes simplex virus [195], Epstein-Barr virus [196], measles [197], and mumps [198], although all of these viruses can also be detected by raised IgG antibodies to each virus. Some viruses, however, can only be detected directly, such as the SARS (severe acute respiratory syndrome) coronavirus [199], rabies virus [200], and Ebola [201], the latter of which has a very high mortality rate consisting mainly of antibody-negative patients [202].…”

Section: Detection Of Systemic Infections In Salivamentioning

confidence: 99%

Saliva: Physiology and Diagnostic Potential in Health and Disease

Farnaud

Kosti

Getting

et al. 2010

The Scientific World JOURNAL

View full text Add to dashboard Cite

Saliva has been described as the mirror of the body. In a world of soaring healthcare costs and an environment where rapid diagnosis may be critical to a positive patient outcome, saliva is emerging as a viable alternative to blood sampling. In this review, we discuss the composition and various physiological roles of saliva in the oral cavity, including soft tissue protection, antimicrobial activities, and oral tissue repair. We then explore saliva as a diagnostic marker of local oral disease and focus particularly on oral cancers. The cancer theme continues when we focus on systemic disease diagnosis from salivary biomarkers. Communicable disease is the focus of the next section where we review the literature relating to the direct and indirect detection of pathogenic infections from human saliva. Finally, we discuss hormones involved in appetite regulation and whether saliva is a viable alternative to blood in order to monitor hormones that are involved in satiety.

show abstract

Section: Detection Of Systemic Infections In Salivamentioning

confidence: 99%

Saliva: Physiology and Diagnostic Potential in Health and Disease

Farnaud

Kosti

Getting

et al. 2010

The Scientific World JOURNAL

View full text Add to dashboard Cite

show abstract

“…However, cases can appear in vaccinated people [30], especially in those who have received only one dose of vaccine [31]. Cases in vaccinated subjects increase with age [32][33][34].…”

Section: Discussionmentioning

confidence: 99%

Community Outbreak of Measles in Madrid (Spain) Caused by an Imported Case~!2009-11-06~!2009-11-10~!2010-02-16~!

Comas¹,

Gavín²,

Moreno³

et al. 2010

TOVACJ

View full text Add to dashboard Cite

Introduction: In February 2006, the measles elimination programme of the Epidemiological Surveillance Network of the Community of Madrid (Spain) detected a case of imported, unvaccinated measles which was the index case of a community outbreak whose epidemiological characteristics we describe. Material and Methods: Descriptive study of the outbreak. Clinical, epidemiological and laboratory information was collected. Measles antibodies (IgM and Ig G) were studied and the virus was isolated and genotyped. Results: An imported case caused an outbreak of 174 cases (93.1% confirmed). The measles virus was detected by culture in 31% of cases. The genotype was determined in 90.7% of positive samples, all of which were the B3 genotype (subgenotype B3.1), with sequencing in various genes that indicated the same viral lineage. The outbreak lasted 198 days. The age range was 2 months-37 years and 84.6% of cases were Spanish-born. A total of 9.8% of cases presented complications, 37.4% required hospital admission, and 9.2% had a documented history of immunization. Twenty-four clusters were identified in families, health workers and infant schools. Conclusions: Control measures included advancing the infant MMR vaccination schedule and reinforcement vaccination in health care workers. While the measles virus still circulates, surveillance of suspected cases and maintenance of high vaccination coverages, especially in health care workers, are essential.

show abstract

“…233 Outbreaks of measles communities such as in Gypsy-Travelers are well recognized. 234 Measles virus is found in saliva, 45,235 where nucleic acid can be amplified by PCR 39 and directly from using a point-of-care test. 236 Measles is usually spread by droplets but kissing may well also transmit the virus.…”

Section: Measles Virusmentioning

confidence: 99%