Use of rhizosphere carbon sources in sole carbon source tests to discriminate soil microbial communities

Campbell, Colin D.; Grayston, Susan J.; Hirst, David

doi:10.1016/s0167-7012(97)00041-9

Cited by 337 publications

(178 citation statements)

References 13 publications

Supporting

Mentioning

171

Contrasting

Unclassified

Order By: Relevance

“…The increase in the analysed indicators in the objects treated with post-floatation lime and wool and containing small amounts of nutrients may have been caused by adaptation of the microorganisms to the culture conditions, which were rich in substrates. As suggested by Campbell et al (1997), the differences in the content of the substrates between the culture conditions and the analysed soil may result in different microbial activities in these dissimilar environments. Investigations conducted by other authors (Fierer et al, 2007;Hattori and Hattori, 1980;Joniec et al, 2015) have shown that microorganisms with low nutritional requirements proliferate intensively in a nutrient-rich environment.…”

Section: Discussionmentioning

confidence: 98%

“…As suggested by Campbell et al (1997), the differences in the utilisation of substrates between culture conditions and the analysed soil may result in differences in the intensity of microbial activity in these dissimilar environments. Furthermore, Ros et al (2008) argue that Biolog CLPPs do not reflect the functional capacities of the entire soil microbial community, but only reflect the capacities of a very limited subset of microbial genera.…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Microbial functional diversity and enzymatic activity of soil degraded by sulphur mining reclaimed with various waste

Joniec¹,

Frąc²

2017

International Agrophysics

View full text Add to dashboard Cite

The aim of the study was to evaluate microbial functional diversity based on community level physiological profiling and β-glucosidase activity changes in soil degraded by sulphur mining and subjected to reclamation with various waste. The experiment was set up in the area of the former ‘Jeziórko’ Sulphur Mine (Poland), on a soilless substrate with a particle size distribution of slightly loamy sand. The experimental variants included the application of post-flotation lime, sewage sludge and mineral wool. The analyses of soil samples included the assessment of the following microbiological indices: β-glucosidase activity and functional diversity average well color development and richness). The results indicate that sewage sludge did not exert a significant impact on the functional diversity of microorganisms present in the reclaimed soil. In turn, the application of other types of waste contributed to a significant increase in the parameters of total metabolic activity and functional diversity of the reclaimed soil. However, the temporal analysis of the metabolic profile of soil microorganisms demonstrated that a single application of waste did not yield a durable, stable metabolic profile in the reclaimed soil. Still, there was an increase in β-glucosidase activity, especially in objects treated with sewage sludge.

show abstract

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 98%

Microbial functional diversity and enzymatic activity of soil degraded by sulphur mining reclaimed with various waste

Joniec¹,

Frąc²

2017

International Agrophysics

View full text Add to dashboard Cite

show abstract

“…As a consequence, the color development observed cannot be interpreted in terms of the number of utilizers or the metabolic potential of the original microbial community (Haack et al, 1995). Other potential drawbacks of the Biolog method include the fact that the C sources tested are not necessarily those found in soil and they are present in high concentrations (Campbell et al, 1997). Despite these criticisms, Biolog has been used to assess the impacts of various environmental factors on microbial communities (Gremion et al, 2004;Mäder et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

The veterinary antibiotic oxytetracycline and Cu influence functional diversity of the soil microbial community

Kong

Zhu

et al. 2006

Environmental Pollution

217

View full text Add to dashboard Cite

Oxytetracycline reduces the functional diversity of soil microbial community, and the combination of Cu and oxytetracycline leads to a further reduction. AbstractThere are increasing concerns over the effects of veterinary antibiotics and heavy metals in agricultural soils. The widely used veterinary antibiotic oxytetracycline (OTC), Cu and their combination on soil microbial community function were assessed with the Biolog method. The microbial community was extracted from the soil and exposed to a 0.85% sodium chloride solution containing OTC (0, 1, 5, 11, 43, 109 and 217 mM), or Cu (0, 10, 20, 100 and 300 mM), or combination of the two pollutants (OTC 0, 5, 11 mM and Cu 0, 20 mM). Functional diversity, evenness, average well color development (AWCD) and substrate utilization decreased significantly with increasing concentrations of OTC or Cu ( p < 0.005). The critical concentrations were 11 mM for OTC and 20 mM for Cu. The combination of OTC and Cu significantly decreased Shannon's diversity, evenness and utilization of carbohydrates and carboxylic acids compared to individual one of the contaminants. The antibiotic OTC and Cu had significant negative effects on soil microbial community function, particularly when both pollutants were present.

show abstract

“…The shifts in Biolog metabolic diversity patterns are related to shifts in community composition (Haack et al 1995). CLPPs, based on Biolog Ecoplates, have shown to be an appropriate indicator of functional diversity and activity of soil microorganisms in metabolizing carbon substrates (Campbell et al 1997;Chen et al 2010). Although the outcomes reflected only patterns of carbon source utilization during the Ecoplate incubation period and only select metabolic functions were represented in the Ecoplates (Preston-Mafham et al 2002;Zheng et al 2005), CLPPs provide a fast and sensitive way to equally compare functional characteristics of soil microbial communities.…”

Section: Discussionmentioning

confidence: 99%

Non-linear impacts of Eucalyptus plantation stand age on soil microbial metabolic diversity

et al. 2013

View full text Add to dashboard Cite

Purpose Although it is generally accepted that planting exotic plant species alters metabolic function of soil microbial communities, its temporal dynamic is often ignored when evaluating ecological effects of associated land use changes. To investigate the dynamic impacts of successive Eucalyptus planting on carbon metabolic activities of soil microbial communities, we studied community-level physiological profiles of soil microbial communities in different generations of Eucalyptus plantations. Materials and methods We studied community-level physiological profiles of soil microbial communities, using the Biolog™ Ecoplates incubation, in adjacent first (G1), second (G2), third (G3), and fourth (G4) generation Eucalyptus plantations that were, respectively, aged 3, 8, 14, and 19 years in Guangxi province, southern China. We used the 'space-fortime substitution' approach to investigate the impact of stand age of exotic Eucalyptus plantations on carbon metabolic diversity and activities of soil microbial communities. For each Eucalyptus plantation generation, three experimental plots were randomly selected. In each plot, one composite soil sample from 0 to 10 cm in depth was obtained for the analyses. Results and discussion Single carbon source utilization varied with Eucalyptus plantation stand age. Among preselected 31 carbon sources, utilization of 17 carbon sources changed significantly, which was best described by a quadratic function (ten carbon sources) and an exponential function (seven carbon sources). As a result, cumulative averaged metabolic activity and metabolic diversity of soil microbial communities showed quadratic and exponential changes relative to Eucalyptus plantation stand age. The order of cumulative averaged carbon metabolic activity and metabolic diversity were G1>G4, G3>G2 and G1>G2>G3, G4 (p<0.05), respectively. The factors contributing to carbon source utilization structure of soil microbial communities for different stand ages of Eucalyptus plantations were shrub richness, soil organic carbon content, microbial biomass carbon, C-to-N ratio, and N-to-P ratio. Conclusions Eucalyptus plantation stand age has inconsistent non-linear impacts on two aspects of soil microbial metabolic function: (1) quadratic impacts on carbon metabolic efficiency and (2) exponential impacts on carbon metabolic diversity. The decreasing carbon metabolic diversity has no significant impact on carbon metabolic efficiency during successive Eucalyptus plantings. The results show that the importance of assessing long-term impacts of land use changes on soil microbial communities from exotic plantations by quantifying multi-aspect non-linear changes on soil microbial metabolic function.

show abstract

Use of rhizosphere carbon sources in sole carbon source tests to discriminate soil microbial communities

Cited by 337 publications

References 13 publications

Microbial functional diversity and enzymatic activity of soil degraded by sulphur mining reclaimed with various waste

Microbial functional diversity and enzymatic activity of soil degraded by sulphur mining reclaimed with various waste

The veterinary antibiotic oxytetracycline and Cu influence functional diversity of the soil microbial community

Non-linear impacts of Eucalyptus plantation stand age on soil microbial metabolic diversity

Contact Info

Product

Resources

About