Use of Rep-PCR for Genetic Diversity Analyses inFusarium culmorum

Abstract

Fusarium culmorum is a pathogen of economically important grain crops. In this work, Rep-PCR was used to identify genetic diversity in F. culmorum isolates which have been collected from wheat fields in Turkey. Reproducible genomic fingerprints were amplified in each strain by PCRs of prokaryotic repetitive extragenic palindromic (REP), enterobacterial repetitive intergenic consensus (ERIC) and BOX sequences. Totally 104 molecular markers were evaluated and similarity comparisons were shown as a dendrogram. The average genetic diversity was 52.3% ranging from 15.8% to 88.7% according to the Rep-PCR data. Cluster analysis showed agreement with the distance of sampling locations. The highest genetic similarity (84.2%) was determined between two F. culmorum isolates (F1 and F2) originated from the same agro-ecological region. Our results showed that Rep-PCR is convenient and rapid for genetic diversity analyses and strain differentiation in F. culmorum.

Morphological, pathological and genetic variations among isolates of Cochliobolus sativus from Nepal

“…However, rep-PCR has an advantage over RAPD for its rapidity and reproducibility in analyzing fungal populations (Mehta et al 2002). These repetitive sequences have been initially used to characterize variability at inter and intraspecific levels in bacterial and fungal pathogens (Adhikari et al 1999;Alves et al 2004;Edel et al 1995;Gurel et al 2010;McDonald et al 2000;Muiru et al 2010;Purkayastha et al 2008). The IS-PCR and rep-PCR assays used in this study also were promising to investigate the genetic diversity of C. sativus.…”

Genetic characterization of Fusarium graminearum and F. culmorum isolates from Turkey by using random-amplified polymorphic DNA

“…Moreover, sexual or parasexual recombination is responsible for intraspecific variation within F. graminearum isolates, whereas asexual or parasexual recombination accounts for variability within F. culmorum isolates (Miedaner et al, 2001). Gürel et al (2010) reported relatively high genetic variability (11.3 to 84.2%) within F. culmorum isolates from Turkey by using repetitive DNA-based fingerprinting methods. Some F. culmorum isolates examined in the present study were also used by Gürel et al (2010).…”

“…Gürel et al (2010) reported relatively high genetic variability (11.3 to 84.2%) within F. culmorum isolates from Turkey by using repetitive DNA-based fingerprinting methods. Some F. culmorum isolates examined in the present study were also used by Gürel et al (2010). A comparison of repetitive element palindromic and RAPD dendrograms has shown that F2, F3, F4, F10, and F14 cluster in the same subgroup, whereas F15, F19, F20, and F21 cluster in the other subgroup.…”

“…The authors also reported that F. graminearum was the primary pathogen responsible for FHB and that the disease incidence was positively correlated with the content of deoxynivalenol that accumulated during that period of time. Gürel et al (2010) determined the genetic variation of 15 Turkish F. culmorum isolates from nine agro-ecological regions using repetitive DNA-based fingerprinting methods. This report was the first to describe the molecular characterization of these isolates and their relationship with the wide geographic distribution of that species.…”

Genetic Diversity Study of Fusarium culmorum: Causal agent of wheat crown rot in Iraq

“…Genetic diversity analyses of microorganisms have demonstrated that pathogen diversity depends on global environmental changes and shifts in agro-ecological systems (Saharan and Naef 2008; Gurel et al 2010). F. culmorum isolates show high levels of phenotypic and genotypic variability in culture, including colony morphology, pigmentation and sporulation (Puhalla 1981; Kollers et al 2013; Miedaner et al 2013).…”