Use of Reflectometer Optipen to Assess the Preventive Effect of a Sugarcane Cystatin on Initial Dental Erosion in Vivo

Hemoglobin Protects Enamel against Intrinsic Enamel Erosive Demineralization

“…Acquired pellicle engineering with acid-resistant proteins has emerged as an innovative and promising tool to prevent dental caries [18] and ETW [13, 19, 20]. Various studies, utilizing in vitro [21‒24], in situ [19], and in vivo [13, 20, 25] protocols, have demonstrated the potential of CaneCPI-5, StN15, and HB in reducing dental demineralization.…”

“…Acquired pellicle engineering with acid-resistant proteins has emerged as an innovative and promising tool to prevent dental caries [18] and ETW [13, 19, 20]. Various studies, utilizing in vitro [21‒24], in situ [19], and in vivo [13, 20, 25] protocols, have demonstrated the potential of CaneCPI-5, StN15, and HB in reducing dental demineralization. However, before dental products containing these proteins/peptide can be widely employed to manage dental caries and/or ETW, a deeper understanding of their mechanism of action and additional evidence on their efficacy, particularly from studies that closely mimic clinical conditions, are needed.…”

“…The in vivo protocol used in this study fulfills this requirement. It was initially developed (and proven effective) for evaluating the capability of these proteins/peptide to alter the AEP’s proteome and to lessen initial extrinsic dental enamel erosion (induced by citric acid) [13, 20]. In this study, we focused on an intrinsic erosive challenge, induced by an enamel biopsy of central incisors with 0.01 m HCl, pH 2.0, for 10 s. It appears that in acquired pellicle engineering with proteins/peptides, there is an acid-specific protective effect offered by the proteins.…”