Use of RAPD-ALF analysis for investigating the frequency of bacterial cross-transmission in an adult intensive care unit

Webster, Carol; Towner, K. J.

doi:10.1053/jhin.1999.0703

Cited by 25 publications

(10 citation statements)

References 18 publications

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“…Several methods have been described for typing S. marcescens during an outbreak, including phenotyping and genotyping methods [16][17][18][19][20]. Some groups [9,21] used PFGE as a highly discriminatory and reproducible method for the epidemiological investigation of S. marcescens infections.…”

Section: Discussionmentioning

confidence: 99%

Nosocomial Neonatal Outbreak of Serratia marcescens - Analysis of Pathogens by Pulsed Field Gel Electrophoresis and Polymerase Chain Reaction

Steppberger¹,

Walter²,

Claros³

et al. 2002

Infection

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This survey shows that PCR and PFGE are comparable in respect to the discrimination and reproducibility for epidemiological studies of S. marcescens strains in nosocomial outbreaks. Genotypic fingerprinting of bacterial isolates is useful and important to limit nosocomial infections. Fingerprinting sources of nosocomial infections can be traced both by PFGE and PCR. All patients infected recovered completely and the nosocomial outbreak could be stopped rapidly.

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Section: Discussionmentioning

confidence: 99%

Nosocomial Neonatal Outbreak of Serratia marcescens - Analysis of Pathogens by Pulsed Field Gel Electrophoresis and Polymerase Chain Reaction

Steppberger¹,

Walter²,

Claros³

et al. 2002

Infection

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“…the frequency of cross-transmission varies between 13 and 34.6%, and is notably high in ICUs. 8,9 A single hand contact with a contaminated surface results in a variable degree of pathogen transfer. Because compliance with hand hygiene is low worldwide, 10 risk from contaminated surfaces is important.…”

Section: Disinfectants Testedmentioning

confidence: 99%

Comparative analysis of newly introduced disinfectants in hospitals in India: An important aspect of infection control policy

Banerjee¹,

Filgona²,

Anupurba³

2013

Int. j. infect. control

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the effective use of disinfectants constitute an important factor in preventing hospital acquired infections. this study was undertaken to compare the effects of three newly introduced disinfectants in a number of tertiary care hospitals in India, namely superoxidized water (SOW) and two quaternary ammonium compounds (QACs), against the effects of 70% ethyl alcohol as one of the most commonly used disinfectants. All the disinfectants were tested for their effectiveness against multidrug resistant (MDr) Pseudomonas aeruginosa, meticillin resistant Staphylococcus aureus (MrSA) and vancomycin resistant enterococci (Vre). Suspension of ≥10 6 bacteria was subjected to the action of different disinfectants for different length of time and in presence and absence of organic matter. reduction in colony count was noted by semiquantitative surface viable colony count method. results were expressed as percentage reductions and analyzed statistically. SOW showed good activity against most of the isolates, except against MrSA in presence of organic matter, where QACs were better. the disinfectants varied in their effects towards different organisms in different environmental conditions and with varying exposure time. therefore, disinfection policies should take into consideration the type of ward and surfaces to be disinfected along with reasons for disinfection for an effective step towards infection control.

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“…Brie¯y, oligonucleotides of about 10 base pairs with an arbitrary chosen sequence are used in a PCR with low stringency resulting in amplicons of various sizes. Comparison of patterns obtained with different primers has been used in epidemiological studies, for example in a study to investigate the frequency of bacterial cross-transmission (Webster et al 2000) or to trace the chain of transmission in a hospital outbreak of extended-spectrum betalactamase-producing Klebsiella pneumoniae (Shannon et al, 1998). The RAPD analysis has also been applied to investigate whether an infection is relapsing (Power, 1996).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Closely Related Orthopoxvirus Isolates by Random Amplified Polymorphic DNA and Restriction Fragment Length Polymorphism Analysis

Stemmler¹,

Neubauer²,

Meyer³

2001

J Vet Med Series B

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The reliability and reproducibility of random amplified polymorphic DNA analysis (RAPD) was compared with restriction fragment length polymorphism (RFLP) by analysing three virus strains isolated from zoo animals in Berlin and three isolates which were cultivated from pets from Northern Germany. The RAPD technique was evaluated as a reliable tool with good reproducibility of the patterns for each virus strain investigated. Problems of interpretation due to inconsistent intensity of bands in different polymerase chain reaction runs may arise for less experienced personnel. The RAPD analysis can be performed within one working day and needs less DNA compared with RFLP so costs will be reduced. The obvious advantage of RFLP is that the pattern can be traced to the recognition site of the restriction enzyme whereas the RAPD primer sequence is not present in the orthopoxvirus genome at all. To the authors knowledge, the RAPD technique has never been applied in DNA viruses before and they conclude that this technique is a useful tool for the discrimination of closely related cowpoxviruses.

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Use of RAPD-ALF analysis for investigating the frequency of bacterial cross-transmission in an adult intensive care unit

Cited by 25 publications

References 18 publications

Nosocomial Neonatal Outbreak of Serratia marcescens - Analysis of Pathogens by Pulsed Field Gel Electrophoresis and Polymerase Chain Reaction

Nosocomial Neonatal Outbreak of Serratia marcescens - Analysis of Pathogens by Pulsed Field Gel Electrophoresis and Polymerase Chain Reaction

Comparative analysis of newly introduced disinfectants in hospitals in India: An important aspect of infection control policy

Comparison of Closely Related Orthopoxvirus Isolates by Random Amplified Polymorphic DNA and Restriction Fragment Length Polymorphism Analysis

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