Use of PrimeStore® Molecular Transport Medium and Xpert MTB/RIF Ultra for Rapid Detection of Mycobacterium Bovis in African buffaloes (Syncerus Caffer).

Clarke, Charlene; Smith, Katrin; Goldswain, Samantha; Helm, Christopher; Cooper, David; Kerr, Tanya J.; Kleynhans, Léanie; Helden, Paul D. van; Warren, Robin M.; Miller, Michele A.; Goosen, Wynand J.

doi:10.21203/rs.3.rs-153226/v1

Cited by 1 publication

(1 citation statement)

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“…Clarke et al investigated an in-field sampling technique for rapid, safe detection of M. bovis in buffalo tissues [ 121 ]. The authors recommended the use of PrimeStore ® Molecular Transport Medium, in combination with Xpert ® MTB/RIF Ultra assay (an automated cartridge-based semi-quantitative nested RT-PCR assay that detects DNA of MBTC and rifampicin (RIF) resistance in clinical specimens) as a safe and rapid PM screening test for M. bovis in buffaloes.…”

Section: Diagnosis Of Btbmentioning

confidence: 99%

Review on Bovine Tuberculosis: An Emerging Disease Associated with Multidrug-Resistant Mycobacterium Species

Borham¹,

Oreiby

El-Gedawy³

et al. 2022

Pathogens

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Bovine tuberculosis is a serious infectious disease affecting a wide range of domesticated and wild animals, representing a worldwide economic and public health burden. The disease is caused by Mycobacteriumbovis and infrequently by other pathogenic mycobacteria. The problem of bovine tuberculosis is complicated when the infection is associated with multidrug and extensively drug resistant M. bovis. Many techniques are used for early diagnosis of bovine tuberculosis, either being antemortem or postmortem, each with its diagnostic merits as well as limitations. Antemortem techniques depend either on cellular or on humoral immune responses, while postmortem diagnosis depends on adequate visual inspection, palpation, and subsequent diagnostic procedures such as bacterial isolation, characteristic histopathology, and PCR to reach the final diagnosis. Recently, sequencing and bioinformatics tools have gained increasing importance for the diagnosis of bovine tuberculosis, including, but not limited to typing, detection of mutations, phylogenetic analysis, molecular epidemiology, and interactions occurring within the causative mycobacteria. Consequently, the current review includes consideration of bovine tuberculosis as a disease, conventional and recent diagnostic methods, and the emergence of MDR-Mycobacterium species.

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Section: Diagnosis Of Btbmentioning

confidence: 99%