Use of prepuberal heifers as oocyte donors for IVF: Effect of age and gonadotrophin treatment

Looney, C.R.; Damiani, P.; Lindsey, B.R.; Long, Cheng; Gonseth, C.L.; Johnson, Dallas; Duby, R.T.

doi:10.1016/0093-691x(95)92423-7

Cited by 40 publications

(16 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has been clearly established that it is possible to generate normal offspring from calf oocytes (Armstrong et al, 1992(Armstrong et al, , 1997Kajihara et al, 1991;Revel et al, 1995). However, the efficiency of this process in comparison with the results obtainable with adult oocytes is reduced Khatir et al, 1996;Looney et al, 1995;Palma, 1994;Palma et al, 1993;Presicce et al, 1997;Revel et al, 1995), therefore suggesting an inherent lack of developmental competence of calf oocytes. In this study it was possible to isolate nearly five times more COCs from calf than from adult ovaries, confirming that even in the absence of exogenous hormonal stimulation, a large number of vesicular follicles are present long before the onset of puberty, as described previously (Desjardins and Hafs, 1969;Erickson, 1966).…”

Section: Discussionmentioning

confidence: 99%

“…In fact, the capability to form blastocysts at a rate equivalent to that of adult oocytes has been reported for young calves (6-9 weeks of age) treated with follicle-stimulating hormone (FSH) (Armstrong et al, 1992(Armstrong et al, , 1994Irvin et al, 1993). On the contrary, a defective developmental competence has been reported in several other studies involving, overall, a much greater number of animals, of a wider range of ages, stimulated or not, with gonadotropins Duby et al, 1996;Khatir et al, 1996;Looney et al, 1995;Palma, 1994;Palma et al, 1993;Presicce et al, 1997;Revel et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative analysis of calf and cow oocytes during in vitro maturation

et al. 1998

View full text Add to dashboard Cite

To determine possible causes of reported differences between developmental competence of oocytes isolated from prepubertal (10‐ to 14‐week‐old calves) and adult cows, three parameters were analysed, comparatively, during in vitro maturation (IVM): (1) oocyte diameter, (2) oocyte energy metabolism, and (3) protein synthesis of oocytes and cumulus cells. Cumulus‐oocyte complexes were isolated from follicles of 3–5 mm in diameter in both age groups. Mean oocyte diameter was smaller (P < 0.02) in calves than in cows (118.04 ± 1.15 versus 122.83 ± 0.74 μm). During the first 3 hr of IVM, calf oocytes metabolised glutamine and pyruvate at lower rates than adult oocytes, but after 24 hr of culture, both molecules were metabolised at the same rate as for adult oocytes. A significant decrease in protein synthesis, as measured by [35S]methionine and [35S]cysteine incorporation was recorded after 9 hr of IVM in calf oocytes, while in adult oocytes a significant decrease in protein synthesis was detected only after 24 hr. After the first 3 hr of maturation, proteins of 130, 26, and 24 kDa were more abundant in adult than in calf oocytes, while a protein of 55 kDa was more visible in calf than in adult oocytes. At the same time, among proteins newly synthesised by cumulus cells, molecules of 405, 146, 101, and 77 kDa were more abundant in adults than in calves. In conclusion, calf oocytes and cumulus cells showed several differences when compared with their adult counterparts, which are consistent with their reported lower developmental competence. Mol. Reprod. Dev. 49:168–175, 1998. © 1998 Wiley‐Liss, Inc.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative analysis of calf and cow oocytes during in vitro maturation

et al. 1998

View full text Add to dashboard Cite

show abstract

“…The production of viable embryos following the in vitro fertilization of oocytes matured in vivo or in vitro has been reported for prepubertal cattle (Armstrong et al 1994), sheep (Earl et al 1995) and goats (Mogas et al 1995). Several researchers have shown that fewer embryos develop in vitro from oocytes derived from prepubertal animals compared with adult animals (pigs: Pinkert et al 1989; cattle: Palma et al 1993;Looney et al 1995;Revel et al 1995). Furthermore, differences in protein patterns and content (LCvesque and Sirard 1994) and sensitivity of Ca2+-release mechanisms Duby et al 1996) have been observed between oocytes obtained from prepubertal and adult cows matured in vitro.…”

Section: Introductionmentioning

confidence: 99%

Developmental capacity, energy metabolism and ultrastructure of mature oocytes from prepubertal and adult sheep

O’Brien

Dwarte²,

Ryan³

et al. 1996

Reprod. Fertil. Dev.

127

View full text Add to dashboard Cite

Development to the blastocyst stage was assessed for oocytes obtained from prepubertal and adult sheep matured and fertilized in vitro. The proportion of cleaved oocytes reaching the blastocyst stage was significantly lower for oocytes derived from prepubertal sheep than for those from adult sheep (7.4% and 24.6% respectively). There were no differences in the metabolism of glucose, glutamine or pyruvate between oocytes matured in vivo and in vitro, or of glucose or pyruvate between oocytes from prepubertal and adult sheep. Glutamine metabolism by mature oocytes from prepubertal sheep was significantly lower than that by oocytes from adult sheep. Ultrastructural studies revealed no differences in the morphology of cytoplasmic organelles of oocytes matured in vitro from prepubertal and adult sheep, but differences in the volume fraction and size of mitochondria and cortical granules were observed. These data suggest that mature oocytes from prepubertal sheep do not possess the developmental potential of their adult-derived counterparts, and this phenomenon may be associated with metabolic and ultrastructural anomalies.

show abstract

“…Ovaries of prepuberal calves exhibit a wavelike pattern of follicular growth, which is similar to those of postpubertal animals Evans et al, 1994). Collection of these immature calf oocytes by follicular aspiration, followed by in vitro maturation, fertilization, and culture (NMFC), has shown that these oocytes can resume meiosis, undergo germinal vesicle breakdown (GVBD), reach metaphase I1 (MII), and be fertilized (Armstrong et al, 1992(Armstrong et al, , 1994Duby et al, 1995;Looney et al, 1995;Precisse et al, 1995). However, when cultured, their rates of cleavage and development have been consistently lower than for postpubertal animals (Kajihara et al, 1991;Palma et al, 1993;Lkvesque et al, 1994;Duby et al, 1995Duby et al, , 1996Looney et al, 1995;Revel et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of developmental competence, nuclear and ooplasmic maturation of calf oocytes

Damiani¹,

Fissore²,

Cibelli³

et al. 1996

Mol. Reprod. Dev.

Self Cite

View full text Add to dashboard Cite

In this study we evaluated nuclear and ooplasmic maturation of prepuberal calf oocytes to determine a possible cause for their low developmental competency. Calf oocytes resumed meiosis and arrested at the MII stage at rates similar to that of adult animals; however, zygotes derived from calf oocytes cleaved and developed at significantly lower rates. Ooplasmic maturation was assessed during oocyte maturation and fertilization. Transmission electron microscopy revealed that a majority of calf oocytes exhibited some delay in organelle migration and redistribution following maturation. Immunofluorescence microscopy showed that following IVF, a higher percentage of calf oocytes had abnormal chromatin and microtubule configurations than those of adult cattle. These anomalies were characterized by delayed formation of sperm aster and asynchronous pronuclear formation. Microfluorometry was used to characterize the Ca2+ responses of calf oocytes to the addition of agonists or after IVF. The addition of thimerosal demonstrated the presence of Ca2+ stores in calf oocytes. Injection of near threshold concentrations of inositol 1,4,5-trisphosphate (InsP3), used to test the sensitivity of the InsP3R, released significantly less Ca2+ in calf than in cow oocytes, whereas higher concentrations of InsP3 (500 microM) released maximal [Ca2+]i in both oocytes. These results suggested that the Ca2+ content of intracellular stores was similar, but the sensitivity of the InsP3R may be different. Following insemination, calf oocytes exhibiting [Ca2+]i oscillations displayed comparable amplitude and intervals to cow oocytes; however, a significantly higher number of fertilized calf oocytes failed to show oscillations. Our findings suggest that the low developmental competence of calf oocytes can be attributed, at least in part, to incomplete or delayed ooplasmic maturation.

show abstract

Use of prepuberal heifers as oocyte donors for IVF: Effect of age and gonadotrophin treatment

Cited by 40 publications

References 0 publications

Comparative analysis of calf and cow oocytes during in vitro maturation

Comparative analysis of calf and cow oocytes during in vitro maturation

Developmental capacity, energy metabolism and ultrastructure of mature oocytes from prepubertal and adult sheep

Evaluation of developmental competence, nuclear and ooplasmic maturation of calf oocytes

Contact Info

Product

Resources

About