2012
DOI: 10.1371/journal.pone.0045282
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Use of Postmortem Human Dura Mater and Scalp for Deriving Human Fibroblast Cultures

Abstract: Fibroblasts can be collected from deceased individuals, grown in culture, reprogrammed into induced pluripotent stem cells (iPSCs), and then differentiated into a multitude of cell types, including neurons. Past studies have generated iPSCs from somatic cell biopsies from either animal or human subjects. Previously, fibroblasts have only been successfully cultured from postmortem human skin in two studies. Here we present data on fibroblast cell cultures generated from 146 scalp and/or 53 dura mater samples fr… Show more

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Cited by 31 publications
(36 citation statements)
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References 15 publications
(19 reference statements)
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“…The genes with differences in expression and DNAm levels by sampling location were previously implicated in processes relating to cell proliferation and apoptosis, which likely relate to the function of the fibroblasts in the primary tissue. One might have predicted this outcome, as fibroblasts in the scalp, including those that are cultured, turnover much more rapidly than those in the dura mater [ 15 ], which we confirmed here with increased FSP-1 expression in the scalp-derived fibroblasts.…”
Section: Discussionsupporting
confidence: 77%
See 3 more Smart Citations
“…The genes with differences in expression and DNAm levels by sampling location were previously implicated in processes relating to cell proliferation and apoptosis, which likely relate to the function of the fibroblasts in the primary tissue. One might have predicted this outcome, as fibroblasts in the scalp, including those that are cultured, turnover much more rapidly than those in the dura mater [ 15 ], which we confirmed here with increased FSP-1 expression in the scalp-derived fibroblasts.…”
Section: Discussionsupporting
confidence: 77%
“…Differential expression analysis of the RNA-seq data, independent of the results from the epigenetic analyses above, identified many genes that differed by the source of the primary fibroblast– 5,830 genes at FDR < 5%. Both scalp- and dura-derived fibroblasts expressed high levels of Fibroblast Specific Protein-1 (FSP-1) and this gene was more highly expressed scalp-derived fibroblasts (fold change = 5.5, FDR = 5.6x10 -6 ) in line with increased higher proliferation rates in the scalp-derived versus dura-derived fibroblasts [ 15 ]. The differentially expressed genes were strongly enriched for signaling and cell communication, cell proliferation, apoptotic processes, and epithelium development and morphogenesis via gene ontology (GO) analysis (all p < 10 −8 , S4 Table )–these gene sets were similar, and much more significant, to those identified comparing gene expression profiles across positional-identity genes in dermal fibroblasts [ 17 ].…”
Section: Resultsmentioning
confidence: 99%
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“…Another advantage is the ability to derive iPSCs from different cellular sources. iPSCs can be reprogrammed from patientderived cell sources, including blood [15][16][17][18], skin fibroblasts [19,20], hair follicles [21], dental tissue [22], urine [23], and even postmortem tissue [24][25][26]. Another unique consideration is that patient cellular sources can be collected across a lifetime to examine the effects of the environment and age on the differentiation ability over time.…”
Section: Do Ipsc-derived Cells Resolve Some Of These Issues?mentioning
confidence: 99%